SLD5_ARATH
ID SLD5_ARATH Reviewed; 220 AA.
AC Q6NQP5; Q9FI69;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=DNA replication complex GINS protein SLD5 {ECO:0000303|PubMed:17556508, ECO:0000303|PubMed:28428341};
DE AltName: Full=Protein EMBRYO DEFECTIVE 2812 {ECO:0000303|PubMed:31334862};
DE AltName: Full=Protein SYNTHETIC LETHALITY WITH DPB11-1 5 {ECO:0000303|PubMed:17556508, ECO:0000303|PubMed:28428341};
GN Name=SLD5 {ECO:0000303|PubMed:17556508, ECO:0000303|PubMed:28428341};
GN Synonyms=EMB2812 {ECO:0000303|PubMed:31334862};
GN OrderedLocusNames=At5g49010 {ECO:0000312|Araport:AT5G49010};
GN ORFNames=K19E20.14 {ECO:0000312|EMBL:BAB10325.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP REVIEW.
RX PubMed=17556508; DOI=10.1104/pp.107.101105;
RA Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.;
RT "Genome-wide analysis of the core DNA replication machinery in the higher
RT plants Arabidopsis and rice.";
RL Plant Physiol. 144:1697-1714(2007).
RN [6]
RP DEVELOPMENTAL STAGE.
RX PubMed=22664253; DOI=10.1016/j.ygeno.2012.05.013;
RA Xu K., Liu J., Fan M., Xin W., Hu Y., Xu C.;
RT "A genome-wide transcriptome profiling reveals the early molecular events
RT during callus initiation in Arabidopsis multiple organs.";
RL Genomics 100:116-124(2012).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22164284; DOI=10.1371/journal.pone.0028398;
RA Muralla R., Lloyd J., Meinke D.;
RT "Molecular foundations of reproductive lethality in Arabidopsis thaliana.";
RL PLoS ONE 6:e28398-e28398(2011).
RN [8]
RP INTERACTION WITH EOL1.
RC STRAIN=cv. Columbia;
RX PubMed=28428341; DOI=10.1073/pnas.1620955114;
RA Zhou Y., Tergemina E., Cui H., Foerderer A., Hartwig B.,
RA Velikkakam James G., Schneeberger K., Turck F.;
RT "Ctf4-related protein recruits LHP1-PRC2 to maintain H3K27me3 levels in
RT dividing cells in Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:4833-4838(2017).
RN [9]
RP REVIEW ON EMBRYO-DEFECTIVE MUTANTS.
RX PubMed=31334862; DOI=10.1111/nph.16071;
RA Meinke D.W.;
RT "Genome-wide identification of EMBRYO-DEFECTIVE (EMB) genes required for
RT growth and development in Arabidopsis.";
RL New Phytol. 226:306-325(2020).
CC -!- FUNCTION: The GINS complex plays an essential role in the initiation of
CC DNA replication (PubMed:17556508). Required during embryogenesis
CC (PubMed:22164284). {ECO:0000269|PubMed:22164284,
CC ECO:0000303|PubMed:17556508}.
CC -!- SUBUNIT: Component of the GINS complex (By similarity). Interacts with
CC EOL1 in the nucleus (PubMed:28428341). {ECO:0000250|UniProtKB:Q9BRT9,
CC ECO:0000269|PubMed:28428341}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q99LZ3}.
CC -!- DEVELOPMENTAL STAGE: Expressed in callus 96 hours after initiation on
CC callus-inducing medium. {ECO:0000269|PubMed:22664253}.
CC -!- DISRUPTION PHENOTYPE: Embryo defective. {ECO:0000269|PubMed:22164284}.
CC -!- SIMILARITY: Belongs to the GINS4/SLD5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10325.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Seed defective Arabidopsis mutants;
CC URL="http://seedgenes.org/MutantList";
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DR EMBL; AB017061; BAB10325.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95756.1; -; Genomic_DNA.
DR EMBL; BT010406; AAQ62407.1; -; mRNA.
DR EMBL; AK221044; BAD94796.1; -; mRNA.
DR RefSeq; NP_199712.2; NM_124278.4.
DR AlphaFoldDB; Q6NQP5; -.
DR SMR; Q6NQP5; -.
DR IntAct; Q6NQP5; 2.
DR STRING; 3702.AT5G49010.1; -.
DR PaxDb; Q6NQP5; -.
DR ProteomicsDB; 182945; -.
DR EnsemblPlants; AT5G49010.1; AT5G49010.1; AT5G49010.
DR GeneID; 834960; -.
DR Gramene; AT5G49010.1; AT5G49010.1; AT5G49010.
DR Araport; AT5G49010; -.
DR TAIR; locus:2154329; AT5G49010.
DR eggNOG; KOG3176; Eukaryota.
DR HOGENOM; CLU_071893_1_1_1; -.
DR InParanoid; Q6NQP5; -.
DR OMA; ILETAWI; -.
DR OrthoDB; 1434266at2759; -.
DR PhylomeDB; Q6NQP5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q6NQP5; baseline and differential.
DR GO; GO:0000811; C:GINS complex; ISS:TAIR.
DR GO; GO:0006270; P:DNA replication initiation; TAS:TAIR.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR CDD; cd11711; GINS_A_Sld5; 1.
DR InterPro; IPR021151; GINS_A.
DR InterPro; IPR036224; GINS_bundle-like_dom_sf.
DR InterPro; IPR008591; GINS_Sld5.
DR InterPro; IPR031633; SLD5_C.
DR InterPro; IPR038749; Sld5_GINS_A.
DR PANTHER; PTHR21206; PTHR21206; 1.
DR Pfam; PF05916; Sld5; 1.
DR Pfam; PF16922; SLD5_C; 1.
DR PIRSF; PIRSF007764; Sld5; 1.
DR SUPFAM; SSF158573; SSF158573; 1.
PE 1: Evidence at protein level;
KW DNA replication; Nucleus; Reference proteome.
FT CHAIN 1..220
FT /note="DNA replication complex GINS protein SLD5"
FT /id="PRO_0000455090"
SQ SEQUENCE 220 AA; 25465 MW; A05172637C449131 CRC64;
MASNSEAGGS ADYETLMSTS DVELLKRAWR NEKAAPEILQ YEGALVDRAK EQIELVEETI
EDYVENGIDP LVVSLYQMDL DRAQFLLRSY LRVRLLKIEK FMFHNLNSEE AERRLSEQEK
VFATRCADDL AKHFEETVLL KLPENYQSVL KQSLISEVDD MVPQPHLDTF VVCRSKNFVS
LNLYEEGESP ETVEMERGDL YFIRYKIVKR AIESGQIDLI
