SLD5_MOUSE
ID SLD5_MOUSE Reviewed; 223 AA.
AC Q99LZ3; Q3TP06;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=DNA replication complex GINS protein SLD5;
DE AltName: Full=GINS complex subunit 4;
DE Contains:
DE RecName: Full=DNA replication complex GINS protein SLD5, N-terminally processed;
GN Name=Gins4; Synonyms=Sld5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Heart;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH GINS1, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=16338220; DOI=10.1016/j.bbrc.2005.11.136;
RA Kong L., Ueno M., Itoh M., Yoshioka K., Takakura N.;
RT "Identification and characterization of mouse PSF1-binding protein, SLD5.";
RL Biochem. Biophys. Res. Commun. 339:1204-1207(2006).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: The GINS complex plays an essential role in the initiation of
CC DNA replication, and progression of DNA replication forks. GINS4 is
CC important for GINS complex assembly. GINS complex seems to bind
CC preferentially to single-stranded DNA. {ECO:0000250|UniProtKB:Q9BRT9}.
CC -!- SUBUNIT: Component of the GINS complex which is a heterotetramer of
CC GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with GINS1.
CC GINS complex interacts with DNA primase in vitro (By similarity).
CC Component of the CMG helicase complex, composed of the MCM2-7 complex,
CC the GINS complex and CDC45 (By similarity).
CC {ECO:0000250|UniProtKB:Q7ZT48, ECO:0000250|UniProtKB:Q9BRT9}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16338220}. Chromosome
CC {ECO:0000250|UniProtKB:Q7ZT48}. Cytoplasm
CC {ECO:0000269|PubMed:16338220}.
CC -!- TISSUE SPECIFICITY: Highly abundant in testis. Weakly expressed in
CC thymus and bone marrow. {ECO:0000269|PubMed:16338220}.
CC -!- SIMILARITY: Belongs to the GINS4/SLD5 family. {ECO:0000305}.
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DR EMBL; AK076104; BAC36185.1; -; mRNA.
DR EMBL; AK164815; BAE37931.1; -; mRNA.
DR EMBL; BC002156; AAH02156.1; -; mRNA.
DR CCDS; CCDS22190.1; -.
DR RefSeq; NP_077202.1; NM_024240.6.
DR AlphaFoldDB; Q99LZ3; -.
DR SMR; Q99LZ3; -.
DR BioGRID; 224572; 15.
DR ComplexPortal; CPX-4502; GINS complex.
DR IntAct; Q99LZ3; 1.
DR MINT; Q99LZ3; -.
DR STRING; 10090.ENSMUSP00000033950; -.
DR iPTMnet; Q99LZ3; -.
DR PhosphoSitePlus; Q99LZ3; -.
DR EPD; Q99LZ3; -.
DR MaxQB; Q99LZ3; -.
DR PaxDb; Q99LZ3; -.
DR PeptideAtlas; Q99LZ3; -.
DR PRIDE; Q99LZ3; -.
DR ProteomicsDB; 261420; -.
DR Antibodypedia; 11239; 116 antibodies from 24 providers.
DR DNASU; 109145; -.
DR Ensembl; ENSMUST00000033950; ENSMUSP00000033950; ENSMUSG00000031546.
DR GeneID; 109145; -.
DR KEGG; mmu:109145; -.
DR UCSC; uc009ler.2; mouse.
DR CTD; 84296; -.
DR MGI; MGI:1923847; Gins4.
DR VEuPathDB; HostDB:ENSMUSG00000031546; -.
DR eggNOG; KOG3176; Eukaryota.
DR GeneTree; ENSGT00390000003246; -.
DR HOGENOM; CLU_071893_3_0_1; -.
DR InParanoid; Q99LZ3; -.
DR OMA; ILETAWI; -.
DR OrthoDB; 1434266at2759; -.
DR PhylomeDB; Q99LZ3; -.
DR TreeFam; TF105863; -.
DR Reactome; R-MMU-176974; Unwinding of DNA.
DR BioGRID-ORCS; 109145; 36 hits in 111 CRISPR screens.
DR ChiTaRS; Gins4; mouse.
DR PRO; PR:Q99LZ3; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q99LZ3; protein.
DR Bgee; ENSMUSG00000031546; Expressed in ectoderm and 248 other tissues.
DR Genevisible; Q99LZ3; MM.
DR GO; GO:0071162; C:CMG complex; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0000811; C:GINS complex; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; ISO:MGI.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR GO; GO:0001833; P:inner cell mass cell proliferation; IMP:MGI.
DR GO; GO:1903934; P:positive regulation of DNA primase activity; ISO:MGI.
DR GO; GO:1900264; P:positive regulation of DNA-directed DNA polymerase activity; ISO:MGI.
DR CDD; cd11711; GINS_A_Sld5; 1.
DR InterPro; IPR021151; GINS_A.
DR InterPro; IPR036224; GINS_bundle-like_dom_sf.
DR InterPro; IPR008591; GINS_Sld5.
DR InterPro; IPR031633; SLD5_C.
DR InterPro; IPR038749; Sld5_GINS_A.
DR PANTHER; PTHR21206; PTHR21206; 1.
DR Pfam; PF05916; Sld5; 1.
DR Pfam; PF16922; SLD5_C; 1.
DR PIRSF; PIRSF007764; Sld5; 1.
DR SUPFAM; SSF158573; SSF158573; 1.
PE 1: Evidence at protein level;
KW Acetylation; Chromosome; Cytoplasm; DNA replication; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..223
FT /note="DNA replication complex GINS protein SLD5"
FT /id="PRO_0000421795"
FT INIT_MET 1
FT /note="Removed; alternate"
FT /evidence="ECO:0000250|UniProtKB:Q9BRT9"
FT CHAIN 2..223
FT /note="DNA replication complex GINS protein SLD5, N-
FT terminally processed"
FT /id="PRO_0000327621"
FT REGION 166..223
FT /note="Important for GINS complex assembly"
FT /evidence="ECO:0000250"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9BRT9"
FT MOD_RES 2
FT /note="N-acetylthreonine; in DNA replication complex GINS
FT protein SLD5, N-terminally processed"
FT /evidence="ECO:0000250|UniProtKB:Q9BRT9"
FT MOD_RES 12
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BRT9"
FT MOD_RES 16
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BRT9"
SQ SEQUENCE 223 AA; 25961 MW; A6E1B88E95F6DF1F CRC64;
MTEVLDLHGQ DSDGGSEEMV LTPAELIEKL EQAWMNEKFA PELLESKAEI VECVMEQLEH
MEENLRRAKK GDLKVSIHRM EMERIRYVLS SYLRCRLMKI EKFFPHILEK EKVRSEGEPS
SLSPEEFVFA KEYMDHTETH FKNVALKHMP PNLQKVDLLR AVPKPDLDSY VFLRVKERQE
NILVEPEADE QRDYVIDLEV GSQHLIRYKT IAPLVASGAV QLI
