ID   SLD5_RAT                Reviewed;         223 AA.
AC   Q499W2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=DNA replication complex GINS protein SLD5;
DE   AltName: Full=GINS complex subunit 4;
DE   Contains:
DE     RecName: Full=DNA replication complex GINS protein SLD5, N-terminally processed;
GN   Name=Gins4; Synonyms=Sld5;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Prostate;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12 AND SER-16, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: The GINS complex plays an essential role in the initiation of
CC       DNA replication, and progression of DNA replication forks. GINS4 is
CC       important for GINS complex assembly. GINS complex seems to bind
CC       preferentially to single-stranded DNA. {ECO:0000250|UniProtKB:Q9BRT9}.
CC   -!- SUBUNIT: Component of the GINS complex which is a heterotetramer of
CC       GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with GINS1.
CC       GINS complex interacts with DNA primase in vitro (By similarity).
CC       Component of the CMG helicase complex, composed of the MCM2-7 complex,
CC       the GINS complex and CDC45 (By similarity).
CC       {ECO:0000250|UniProtKB:Q7ZT48, ECO:0000250|UniProtKB:Q9BRT9}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q99LZ3}.
CC       Chromosome {ECO:0000250|UniProtKB:Q7ZT48}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q99LZ3}.
CC   -!- SIMILARITY: Belongs to the GINS4/SLD5 family. {ECO:0000305}.
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DR   EMBL; BC099741; AAH99741.1; -; mRNA.
DR   RefSeq; NP_001025198.1; NM_001030027.1.
DR   AlphaFoldDB; Q499W2; -.
DR   SMR; Q499W2; -.
DR   STRING; 10116.ENSRNOP00000024301; -.
DR   iPTMnet; Q499W2; -.
DR   PhosphoSitePlus; Q499W2; -.
DR   jPOST; Q499W2; -.
DR   PaxDb; Q499W2; -.
DR   GeneID; 290842; -.
DR   KEGG; rno:290842; -.
DR   UCSC; RGD:1307397; rat.
DR   CTD; 84296; -.
DR   RGD; 1307397; Gins4.
DR   eggNOG; KOG3176; Eukaryota.
DR   InParanoid; Q499W2; -.
DR   OrthoDB; 1434266at2759; -.
DR   PhylomeDB; Q499W2; -.
DR   Reactome; R-RNO-176974; Unwinding of DNA.
DR   PRO; PR:Q499W2; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0071162; C:CMG complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0000811; C:GINS complex; ISO:RGD.
DR   GO; GO:0005634; C:nucleus; ISO:RGD.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; ISO:RGD.
DR   GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR   GO; GO:0001833; P:inner cell mass cell proliferation; ISO:RGD.
DR   GO; GO:1903934; P:positive regulation of DNA primase activity; ISO:RGD.
DR   GO; GO:1900264; P:positive regulation of DNA-directed DNA polymerase activity; ISO:RGD.
DR   CDD; cd11711; GINS_A_Sld5; 1.
DR   InterPro; IPR021151; GINS_A.
DR   InterPro; IPR036224; GINS_bundle-like_dom_sf.
DR   InterPro; IPR008591; GINS_Sld5.
DR   InterPro; IPR031633; SLD5_C.
DR   InterPro; IPR038749; Sld5_GINS_A.
DR   PANTHER; PTHR21206; PTHR21206; 1.
DR   Pfam; PF05916; Sld5; 1.
DR   Pfam; PF16922; SLD5_C; 1.
DR   PIRSF; PIRSF007764; Sld5; 1.
DR   SUPFAM; SSF158573; SSF158573; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Chromosome; Cytoplasm; DNA replication; Nucleus;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..223
FT                   /note="DNA replication complex GINS protein SLD5"
FT                   /id="PRO_0000421796"
FT   INIT_MET        1
FT                   /note="Removed; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BRT9"
FT   CHAIN           2..223
FT                   /note="DNA replication complex GINS protein SLD5, N-
FT                   terminally processed"
FT                   /id="PRO_0000327622"
FT   REGION          166..223
FT                   /note="Important for GINS complex assembly"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BRT9"
FT   MOD_RES         2
FT                   /note="N-acetylthreonine; in DNA replication complex GINS
FT                   protein SLD5, N-terminally processed"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BRT9"
FT   MOD_RES         12
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         16
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
SQ   SEQUENCE   223 AA;  26062 MW;  AB1AD1042978AC5B CRC64;
     MTEVQDLHGQ DSDEGSEEVV LTPAELIERL EQAWMNEKFA PELLESKSEI VECVMEQLEH
     MEENLRRAKK GDLKVSIHRM EMERIRYVLS SYLRCRLMKI EKFFPHILEK EKTRPAGEPS
     SLSPEEFVFA KEYMDHTETH FKNVALKHMP PNLQKVDLMR AVPKPDLDSY VFLRVKERQE
     NILVEPEADE QRDYVIDLEE GSQHLIRYKT IAPLVASGAV QLI