ID   SLD5_XENLA              Reviewed;         221 AA.
AC   Q7ZT48;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=DNA replication complex GINS protein SLD5 {ECO:0000305};
DE   AltName: Full=GINS complex subunit 4;
GN   Name=gins4 {ECO:0000312|Xenbase:XB-GENE-949843};
GN   Synonyms=Sld5 {ECO:0000312|EMBL:BAC66457.1};
GN   ORFNames=XELAEV_18018778mg {ECO:0000312|EMBL:OCT90162.1};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, IDENTIFICATION IN THE GINS
RP   COMPLEX, AND SUBCELLULAR LOCATION.
RX   PubMed=12730133; DOI=10.1101/gad.1070003;
RA   Kubota Y., Takase Y., Komori Y., Hashimoto Y., Arata T., Kamimura Y.,
RA   Araki H., Takisawa H.;
RT   "A novel ring-like complex of Xenopus proteins essential for the initiation
RT   of DNA replication.";
RL   Genes Dev. 17:1141-1152(2003).
RN   [2] {ECO:0000312|Proteomes:UP000186698}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=J;
RX   PubMed=27762356; DOI=10.1038/nature19840;
RA   Session A.M., Uno Y., Kwon T., Chapman J.A., Toyoda A., Takahashi S.,
RA   Fukui A., Hikosaka A., Suzuki A., Kondo M., van Heeringen S.J., Quigley I.,
RA   Heinz S., Ogino H., Ochi H., Hellsten U., Lyons J.B., Simakov O.,
RA   Putnam N., Stites J., Kuroki Y., Tanaka T., Michiue T., Watanabe M.,
RA   Bogdanovic O., Lister R., Georgiou G., Paranjpe S.S., van Kruijsbergen I.,
RA   Shu S., Carlson J., Kinoshita T., Ohta Y., Mawaribuchi S., Jenkins J.,
RA   Grimwood J., Schmutz J., Mitros T., Mozaffari S.V., Suzuki Y., Haramoto Y.,
RA   Yamamoto T.S., Takagi C., Heald R., Miller K., Haudenschild C., Kitzman J.,
RA   Nakayama T., Izutsu Y., Robert J., Fortriede J., Burns K., Lotay V.,
RA   Karimi K., Yasuoka Y., Dichmann D.S., Flajnik M.F., Houston D.W.,
RA   Shendure J., DuPasquier L., Vize P.D., Zorn A.M., Ito M., Marcotte E.M.,
RA   Wallingford J.B., Ito Y., Asashima M., Ueno N., Matsuda Y., Veenstra G.J.,
RA   Fujiyama A., Harland R.M., Taira M., Rokhsar D.S.;
RT   "Genome evolution in the allotetraploid frog Xenopus laevis.";
RL   Nature 538:336-343(2016).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   IDENTIFICATION IN THE CMG HELICASE COMPLEX.
RX   PubMed=30979826; DOI=10.26508/lsa.201900390;
RA   Priego Moreno S., Jones R.M., Poovathumkadavil D., Scaramuzza S.,
RA   Gambus A.;
RT   "Mitotic replisome disassembly depends on TRAIP ubiquitin ligase
RT   activity.";
RL   Life. Sci Alliance 2:0-0(2019).
RN   [5]
RP   IDENTIFICATION IN THE CMG HELICASE COMPLEX.
RX   PubMed=30842657; DOI=10.1038/s41586-019-1002-0;
RA   Wu R.A., Semlow D.R., Kamimae-Lanning A.N., Kochenova O.V., Chistol G.,
RA   Hodskinson M.R., Amunugama R., Sparks J.L., Wang M., Deng L., Mimoso C.A.,
RA   Low E., Patel K.J., Walter J.C.;
RT   "TRAIP is a master regulator of DNA interstrand crosslink repair.";
RL   Nature 567:267-272(2019).
CC   -!- FUNCTION: The GINS complex plays an essential role in the initiation of
CC       DNA replication, and progression of DNA replication forks. GINS4 is
CC       important for GINS complex assembly (PubMed:12730133). GINS complex
CC       seems to bind preferentially to single-stranded DNA (By similarity).
CC       {ECO:0000250|UniProtKB:Q9BRT9, ECO:0000269|PubMed:12730133}.
CC   -!- SUBUNIT: Component of the GINS complex which is a heterotetramer of
CC       gins1/psf1, gins2/psf2, gins3/psf3 and gins4/sld5 (PubMed:12730133).
CC       Component of the CMG helicase complex, composed of the mcm2-7 complex,
CC       the GINS complex and cdc45 (PubMed:30979826) (Probable).
CC       {ECO:0000269|PubMed:12730133, ECO:0000269|PubMed:30979826,
CC       ECO:0000305|PubMed:30842657}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12730133}. Chromosome
CC       {ECO:0000269|PubMed:12730133}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q99LZ3}.
CC   -!- SIMILARITY: Belongs to the GINS4/SLD5 family. {ECO:0000305}.
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DR   EMBL; AB097167; BAC66457.1; -; Genomic_DNA.
DR   EMBL; CM004470; OCT90162.1; -; Genomic_DNA.
DR   EMBL; BC068670; AAH68670.1; -; mRNA.
DR   RefSeq; NP_001084702.1; NM_001091233.1.
DR   RefSeq; XP_018106356.1; XM_018250867.1.
DR   RefSeq; XP_018106357.1; XM_018250868.1.
DR   AlphaFoldDB; Q7ZT48; -.
DR   SMR; Q7ZT48; -.
DR   STRING; 8355.Q7ZT48; -.
DR   MaxQB; Q7ZT48; -.
DR   DNASU; 414663; -.
DR   GeneID; 414663; -.
DR   KEGG; xla:414663; -.
DR   CTD; 414663; -.
DR   Xenbase; XB-GENE-949843; gins4.L.
DR   OMA; ILETAWI; -.
DR   OrthoDB; 1434266at2759; -.
DR   Proteomes; UP000186698; Chromosome 3L.
DR   Bgee; 414663; Expressed in ovary and 19 other tissues.
DR   GO; GO:0071162; C:CMG complex; IDA:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000811; C:GINS complex; IPI:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0003682; F:chromatin binding; IDA:UniProtKB.
DR   GO; GO:0006260; P:DNA replication; IMP:UniProtKB.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR   CDD; cd11711; GINS_A_Sld5; 1.
DR   InterPro; IPR021151; GINS_A.
DR   InterPro; IPR036224; GINS_bundle-like_dom_sf.
DR   InterPro; IPR008591; GINS_Sld5.
DR   InterPro; IPR031633; SLD5_C.
DR   InterPro; IPR038749; Sld5_GINS_A.
DR   PANTHER; PTHR21206; PTHR21206; 1.
DR   Pfam; PF05916; Sld5; 1.
DR   Pfam; PF16922; SLD5_C; 1.
DR   PIRSF; PIRSF007764; Sld5; 1.
DR   SUPFAM; SSF158573; SSF158573; 1.
PE   1: Evidence at protein level;
KW   Chromosome; Cytoplasm; DNA replication; Nucleus; Reference proteome.
FT   CHAIN           1..221
FT                   /note="DNA replication complex GINS protein SLD5"
FT                   /id="PRO_0000451425"
SQ   SEQUENCE   221 AA;  25544 MW;  0C4769D5816BD887 CRC64;
     MEDELALSDQ GSDEDEEVLT PAELINKLEE AWLNEKFAPE LLESKSEVVE CVMEQLNHME
     QNLHRAKPGD LKISFHHMEI ERIRYMLSSY LRSRMLKIEK FFPHILEKEK SRGEGEPPHL
     SPEEFAFAKE YMTNTETLLK SVALRHMPPN LQTVDLLKSV PKPNLDSFVF LRVKEEQNNI
     LVEPETDEQS EYAIDMEVGS QHLIRYRTIA PLVASGAVKL I