SLD5_YEAST
ID SLD5_YEAST Reviewed; 294 AA.
AC Q03406; D6VTB2; Q6Q558;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=DNA replication complex GINS protein SLD5;
GN Name=SLD5 {ECO:0000312|SGD:S000002897}; OrderedLocusNames=YDR489W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, COMPOSITION OF THE GINS
RP COMPLEX, SUBCELLULAR LOCATION, AND MUTAGENESIS OF SER-21; SER-66; TRP-67;
RP LYS-150 AND LEU-293.
RX PubMed=12730134; DOI=10.1101/gad.1065903;
RA Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.;
RT "GINS, a novel multiprotein complex required for chromosomal DNA
RT replication in budding yeast.";
RL Genes Dev. 17:1153-1165(2003).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAB64907.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND INTERACTION WITH PSF2.
RX PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT "Assigning function to yeast proteins by integration of technologies.";
RL Mol. Cell 12:1353-1365(2003).
CC -!- FUNCTION: Required for DNA replication. Functions as part of the GINS
CC complex which plays an essential role in the initiation of DNA
CC replication by binding to DNA replication origins and facilitating the
CC assembly of the DNA replication machinery.
CC {ECO:0000250|UniProtKB:P40359, ECO:0000269|PubMed:12730134}.
CC -!- SUBUNIT: Component of the GINS complex which is a heterotetramer
CC composed of SLD5, PSF1, PSF2 and PSF3. Interacts with PSF2.
CC {ECO:0000269|PubMed:14690591}.
CC -!- INTERACTION:
CC Q03406; Q01454: CTF4; NbExp=14; IntAct=EBI-37437, EBI-5209;
CC Q03406; Q12488: PSF1; NbExp=6; IntAct=EBI-37437, EBI-22066;
CC Q03406; P40359: PSF2; NbExp=7; IntAct=EBI-37437, EBI-25936;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12730134}.
CC -!- SIMILARITY: Belongs to the GINS4/SLD5 family. {ECO:0000305}.
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DR EMBL; U33050; AAB64907.1; -; Genomic_DNA.
DR EMBL; AY558492; AAS56818.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12322.1; -; Genomic_DNA.
DR PIR; S69656; S69656.
DR RefSeq; NP_010777.3; NM_001180797.3.
DR PDB; 3JC5; EM; 4.70 A; D=1-294.
DR PDB; 3JC6; EM; 3.70 A; D=1-294.
DR PDB; 3JC7; EM; 4.80 A; D=1-294.
DR PDB; 4C95; X-ray; 2.69 A; D/E=1-19.
DR PDB; 5NXQ; X-ray; 2.41 A; D/E=1-19.
DR PDB; 5U8S; EM; 6.10 A; D=1-294.
DR PDB; 5U8T; EM; 4.90 A; D=1-294.
DR PDB; 6HV9; EM; 4.98 A; F=1-294.
DR PDB; 6PTJ; EM; 3.80 A; D=1-294.
DR PDB; 6PTN; EM; 5.80 A; D/d=1-294.
DR PDB; 6PTO; EM; 7.00 A; D/d/q=1-294.
DR PDB; 6SKL; EM; 3.70 A; D=1-294.
DR PDB; 6U0M; EM; 3.90 A; D=3-293.
DR PDB; 7PMK; EM; 3.20 A; D=1-294.
DR PDB; 7PMN; EM; 3.20 A; D=1-294.
DR PDBsum; 3JC5; -.
DR PDBsum; 3JC6; -.
DR PDBsum; 3JC7; -.
DR PDBsum; 4C95; -.
DR PDBsum; 5NXQ; -.
DR PDBsum; 5U8S; -.
DR PDBsum; 5U8T; -.
DR PDBsum; 6HV9; -.
DR PDBsum; 6PTJ; -.
DR PDBsum; 6PTN; -.
DR PDBsum; 6PTO; -.
DR PDBsum; 6SKL; -.
DR PDBsum; 6U0M; -.
DR PDBsum; 7PMK; -.
DR PDBsum; 7PMN; -.
DR AlphaFoldDB; Q03406; -.
DR SMR; Q03406; -.
DR BioGRID; 32541; 178.
DR ComplexPortal; CPX-1641; GINS complex.
DR DIP; DIP-1813N; -.
DR IntAct; Q03406; 27.
DR MINT; Q03406; -.
DR STRING; 4932.YDR489W; -.
DR MaxQB; Q03406; -.
DR PaxDb; Q03406; -.
DR PRIDE; Q03406; -.
DR EnsemblFungi; YDR489W_mRNA; YDR489W; YDR489W.
DR GeneID; 852100; -.
DR KEGG; sce:YDR489W; -.
DR SGD; S000002897; SLD5.
DR VEuPathDB; FungiDB:YDR489W; -.
DR eggNOG; KOG3176; Eukaryota.
DR GeneTree; ENSGT00390000003246; -.
DR HOGENOM; CLU_071893_2_0_1; -.
DR InParanoid; Q03406; -.
DR OMA; ILETAWI; -.
DR BioCyc; YEAST:G3O-30013-MON; -.
DR Reactome; R-SCE-176974; Unwinding of DNA.
DR PRO; PR:Q03406; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q03406; protein.
DR GO; GO:0071162; C:CMG complex; IDA:SGD.
DR GO; GO:0031261; C:DNA replication preinitiation complex; IMP:SGD.
DR GO; GO:0000811; C:GINS complex; IPI:SGD.
DR GO; GO:0043596; C:nuclear replication fork; IDA:ComplexPortal.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0031298; C:replication fork protection complex; IDA:SGD.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IDA:ComplexPortal.
DR GO; GO:0006261; P:DNA-templated DNA replication; IMP:SGD.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IMP:SGD.
DR GO; GO:1903934; P:positive regulation of DNA primase activity; IC:ComplexPortal.
DR GO; GO:1900264; P:positive regulation of DNA-directed DNA polymerase activity; IC:ComplexPortal.
DR CDD; cd11711; GINS_A_Sld5; 1.
DR InterPro; IPR021151; GINS_A.
DR InterPro; IPR036224; GINS_bundle-like_dom_sf.
DR InterPro; IPR008591; GINS_Sld5.
DR InterPro; IPR031633; SLD5_C.
DR InterPro; IPR038749; Sld5_GINS_A.
DR PANTHER; PTHR21206; PTHR21206; 1.
DR Pfam; PF05916; Sld5; 1.
DR Pfam; PF16922; SLD5_C; 1.
DR PIRSF; PIRSF007764; Sld5; 1.
DR SUPFAM; SSF158573; SSF158573; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA replication; Nucleus; Reference proteome.
FT CHAIN 1..294
FT /note="DNA replication complex GINS protein SLD5"
FT /id="PRO_0000071955"
FT MUTAGEN 21
FT /note="S->P: In sld5-8; temperature-sensitive mutant; in
FT association with P-66. Defective in DNA replication."
FT /evidence="ECO:0000269|PubMed:12730134"
FT MUTAGEN 66
FT /note="S->P: In sld5-8; temperature-sensitive mutant; in
FT association with P-21. Defective in DNA replication."
FT /evidence="ECO:0000269|PubMed:12730134"
FT MUTAGEN 67
FT /note="W->R: In sld5-12; temperature-sensitive mutant.
FT Defective in DNA replication."
FT /evidence="ECO:0000269|PubMed:12730134"
FT MUTAGEN 150
FT /note="K->E: In sld5-2; temperature-sensitive mutant.
FT Defective in DNA replication."
FT /evidence="ECO:0000269|PubMed:12730134"
FT MUTAGEN 293
FT /note="L->P: In sld5-13; temperature-sensitive mutant.
FT Defective in DNA replication."
FT /evidence="ECO:0000269|PubMed:12730134"
FT CONFLICT 232
FT /note="V -> A (in Ref. 4; AAS56818)"
FT /evidence="ECO:0000305"
FT TURN 6..8
FT /evidence="ECO:0007829|PDB:5NXQ"
FT HELIX 56..72
FT /evidence="ECO:0007829|PDB:7PMK"
FT HELIX 81..106
FT /evidence="ECO:0007829|PDB:7PMK"
FT HELIX 124..153
FT /evidence="ECO:0007829|PDB:7PMK"
FT HELIX 155..163
FT /evidence="ECO:0007829|PDB:7PMK"
FT HELIX 171..174
FT /evidence="ECO:0007829|PDB:7PMK"
FT HELIX 177..197
FT /evidence="ECO:0007829|PDB:7PMK"
FT HELIX 199..201
FT /evidence="ECO:0007829|PDB:7PMK"
FT TURN 204..206
FT /evidence="ECO:0007829|PDB:7PMK"
FT STRAND 212..216
FT /evidence="ECO:0007829|PDB:7PMK"
FT STRAND 227..231
FT /evidence="ECO:0007829|PDB:7PMK"
FT STRAND 236..238
FT /evidence="ECO:0007829|PDB:7PMK"
FT TURN 240..242
FT /evidence="ECO:0007829|PDB:7PMK"
FT HELIX 250..252
FT /evidence="ECO:0007829|PDB:7PMK"
FT STRAND 254..260
FT /evidence="ECO:0007829|PDB:7PMK"
FT TURN 261..264
FT /evidence="ECO:0007829|PDB:7PMK"
FT STRAND 265..270
FT /evidence="ECO:0007829|PDB:7PMK"
FT STRAND 274..278
FT /evidence="ECO:0007829|PDB:7PMK"
FT HELIX 280..282
FT /evidence="ECO:0007829|PDB:7PMK"
FT HELIX 283..287
FT /evidence="ECO:0007829|PDB:7PMK"
FT STRAND 290..293
FT /evidence="ECO:0007829|PDB:7PMK"
SQ SEQUENCE 294 AA; 33948 MW; 7EC516ADAD9900DD CRC64;
MDINIDDILA ELDKETTAVD STKITQGSSS TTHRDANTIV GSSLDLNDKT QIYVSPQQDF
SDLMKSWKNE RCSPELLPYP HQLMKRLLNR ISMQSQLIEN ISMGFLDMQN ASNANPPMPN
ESKLPLLCME TELERLKFVI RSYIRCRLSK IDKFSLYLRQ LNEDENSLIS LTDLLSKDEI
KYHDTHSLIW LKLVNDSILK YMPEELQAIN DTEGSVNMID EPDWNKFVFI HVNGPPDGKW
NEDPLLQENE FGKPCYTVTI PDLKEEVELT IGSIYVMRYE VIRDLLRDDK VALI
