SLD7_YEAST
ID SLD7_YEAST Reviewed; 257 AA.
AC Q08457; D6W2C3; O00023;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Mitochondrial morphogenesis protein SLD7;
DE AltName: Full=Synthetic lethality with DPB11-24 mutation protein 7;
GN Name=SLD7; OrderedLocusNames=YOR060C; ORFNames=YOR29-11;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=9133743;
RX DOI=10.1002/(sici)1097-0061(19970330)13:4<379::aid-yea85>3.0.co;2-g;
RA Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.;
RT "The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the
RT presence of two tRNAs and 24 new open reading frames.";
RL Yeast 13:379-390(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP FUNCTION.
RX PubMed=16135527; DOI=10.1091/mbc.e05-07-0678;
RA Altmann K., Westermann B.;
RT "Role of essential genes in mitochondrial morphogenesis in Saccharomyces
RT cerevisiae.";
RL Mol. Biol. Cell 16:5410-5417(2005).
RN [7]
RP FUNCTION, AND INTERACTION WITH SLD3.
RX PubMed=21487389; DOI=10.1038/emboj.2011.115;
RA Tanaka T., Umemori T., Endo S., Muramatsu S., Kanemaki M., Kamimura Y.,
RA Obuse C., Araki H.;
RT "Sld7, an Sld3-associated protein required for efficient chromosomal DNA
RT replication in budding yeast.";
RL EMBO J. 30:2019-2030(2011).
CC -!- FUNCTION: Required for the proper function of SLD3 at the initiation of
CC DNA replication. Binds to SLD3 and reduces its affinity for CDC45, a
CC component of the replication fork. Required for mitochondrial
CC morphology. {ECO:0000269|PubMed:16135527, ECO:0000269|PubMed:21487389}.
CC -!- SUBUNIT: Interacts with SLD3. {ECO:0000269|PubMed:21487389}.
CC -!- INTERACTION:
CC Q08457; P53135: SLD3; NbExp=9; IntAct=EBI-36267, EBI-23925;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}. Cytoplasm,
CC cytoskeleton, spindle pole {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1690 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SLD7 family. {ECO:0000305}.
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DR EMBL; Z70678; CAA94545.1; -; Genomic_DNA.
DR EMBL; Z74968; CAA99253.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10839.1; -; Genomic_DNA.
DR PIR; S66943; S66943.
DR RefSeq; NP_014703.1; NM_001183479.1.
DR PDB; 3X38; X-ray; 1.80 A; A/B/C/D=178-257.
DR PDBsum; 3X38; -.
DR AlphaFoldDB; Q08457; -.
DR SMR; Q08457; -.
DR BioGRID; 34459; 18.
DR DIP; DIP-4168N; -.
DR IntAct; Q08457; 2.
DR MINT; Q08457; -.
DR STRING; 4932.YOR060C; -.
DR MaxQB; Q08457; -.
DR PaxDb; Q08457; -.
DR PRIDE; Q08457; -.
DR EnsemblFungi; YOR060C_mRNA; YOR060C; YOR060C.
DR GeneID; 854226; -.
DR KEGG; sce:YOR060C; -.
DR SGD; S000005586; SLD7.
DR VEuPathDB; FungiDB:YOR060C; -.
DR eggNOG; ENOG502RZIJ; Eukaryota.
DR HOGENOM; CLU_072105_0_0_1; -.
DR InParanoid; Q08457; -.
DR OMA; EFTHRDE; -.
DR BioCyc; YEAST:G3O-33600-MON; -.
DR PRO; PR:Q08457; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q08457; protein.
DR GO; GO:0000775; C:chromosome, centromeric region; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0031261; C:DNA replication preinitiation complex; IDA:SGD.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005635; C:nuclear envelope; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0030174; P:regulation of DNA-templated DNA replication initiation; IGI:SGD.
DR InterPro; IPR016808; Sld7.
DR InterPro; IPR041260; Sld7_C.
DR InterPro; IPR041564; Sld7_N.
DR Pfam; PF18596; Sld7_C; 1.
DR Pfam; PF18636; Sld7_N; 1.
DR PIRSF; PIRSF022788; UCP022788; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cytoplasm; Cytoskeleton; DNA replication;
KW Nucleus; Reference proteome.
FT CHAIN 1..257
FT /note="Mitochondrial morphogenesis protein SLD7"
FT /id="PRO_0000237650"
FT HELIX 182..198
FT /evidence="ECO:0007829|PDB:3X38"
FT HELIX 208..222
FT /evidence="ECO:0007829|PDB:3X38"
FT HELIX 224..231
FT /evidence="ECO:0007829|PDB:3X38"
FT HELIX 240..254
FT /evidence="ECO:0007829|PDB:3X38"
SQ SEQUENCE 257 AA; 29557 MW; 8D79B4B1B8CD9C01 CRC64;
MSRKLCTLNF TLSGKQGSLV IRDIQLWSNR PTASKSTSEL RGQFIQYVDL AKLPLWVRST
NMNTYRCYST SATAQAYFKS KLRNANRGIV IELFDKVDQR SQEPAYLIIF RENTELNCFQ
VDLTMKHEFD GQVTKLKQDI GKTRASVSKE GSIDIIIQQS QQRKIGTKTE VYRNVHINDK
RLQFNETLSK LILGGLRLRG ISNSITDYQK LYKITFDAAE FTHRDELKRI SMGSGEEVSF
ESLQETVETL LKLFTKS
