ID   SLDA_TRIAB              Reviewed;         158 AA.
AC   P0DM38;
DT   29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2013, sequence version 1.
DT   25-MAY-2022, entry version 21.
DE   RecName: Full=Snaclec alboaggregin-D subunit alpha;
DE   Flags: Precursor;
OS   Trimeresurus albolabris (White-lipped pit viper) (Cryptelytrops
OS   albolabris).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Crotalinae; Trimeresurus.
OX   NCBI_TaxID=8765;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=22033027; DOI=10.1016/j.toxicon.2011.10.009;
RA   Mekchay P., Rojnuckarin P.;
RT   "Molecular cloning and characterization of alboaggregin D, a novel platelet
RT   activating protein, from Green pit viper (Cryptelytrops albolabris)
RT   venom.";
RL   Toxicon 59:59-67(2012).
CC   -!- FUNCTION: Snaclec that induces human platelet aggregation in the
CC       absence of any cofactor with the EC(50) of 0.25 nM and causes tyrosine
CC       phosphorylation in human platelets. Antibodies against either platelet
CC       GPIbalpha (GP1BA) or GPVI (GP6) inhibit alboaggregin D-induced platelet
CC       aggregation. Only the combination of these two antibodies completely
CC       inhibit aggregation, suggesting that it acts through both GPIbalpha
CC       (GP1BA) and GPVI (GP6). {ECO:0000269|PubMed:22033027}.
CC   -!- SUBUNIT: Tetramer of heterodimers of alpha and beta subunits
CC       (alphabeta)(4); disulfide-linked. {ECO:0000269|PubMed:22033027}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22033027}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:22033027}.
CC   -!- SIMILARITY: Belongs to the snaclec family. {ECO:0000305}.
CC   -!- CAUTION: Position 67 and 84 are wrongly translated from the nucleotide
CC       sequence, it is why a feature 'unsure' is indicated. AGC (position 67)
CC       code for a Ser but a Val is indicated instead, whereas CTA (position
CC       84) code for a Leu but a Iso is indicated instead. {ECO:0000305}.
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DR   AlphaFoldDB; P0DM38; -.
DR   SMR; P0DM38; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.100.10; -; 1.
DR   InterPro; IPR001304; C-type_lectin-like.
DR   InterPro; IPR016186; C-type_lectin-like/link_sf.
DR   InterPro; IPR018378; C-type_lectin_CS.
DR   InterPro; IPR016187; CTDL_fold.
DR   Pfam; PF00059; Lectin_C; 1.
DR   SMART; SM00034; CLECT; 1.
DR   SUPFAM; SSF56436; SSF56436; 1.
DR   PROSITE; PS00615; C_TYPE_LECTIN_1; 1.
DR   PROSITE; PS50041; C_TYPE_LECTIN_2; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Hemostasis impairing toxin;
KW   Platelet aggregation activating toxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000250"
FT   CHAIN           24..158
FT                   /note="Snaclec alboaggregin-D subunit alpha"
FT                   /id="PRO_0000422608"
FT   DOMAIN          34..153
FT                   /note="C-type lectin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT   DISULFID        27..38
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT   DISULFID        55..152
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT   DISULFID        104
FT                   /note="Interchain (with C-100 in subunit beta of
FT                   heterodimeric partner)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT   DISULFID        127..144
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT   DISULFID        158
FT                   /note="Interchain (with C-26 in subunit beta of tetrameric
FT                   partner)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT   VARIANT         80
FT                   /note="V -> I"
FT   UNSURE          67
FT   UNSURE          84
SQ   SEQUENCE   158 AA;  18017 MW;  8FAB70FDC678C203 CRC64;
     MGRFIFGSFG LLVVFLSLSG TGADFDCPPG WSAYDRYCYQ AFSEPKTWED AESFCMEGVK
     DSHLVSVESS GEADFVAQLV NENIKTSFRY VWIGLRIQNK EQQCRSEWSD ASSVSYENLI
     KKVSKKCYGL KKGTELRTWF NVYCAELNPF ICKFPPEC