ID   SLEB_ALKHC              Reviewed;         330 AA.
AC   Q9KCE0; Q9RC83;
DT   25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Spore cortex-lytic enzyme;
DE            Short=SCLE;
DE   Flags: Precursor;
GN   Name=sleB; OrderedLocusNames=BH1631;
OS   Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344
OS   / JCM 9153 / C-125) (Bacillus halodurans).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus.
OX   NCBI_TaxID=272558;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125;
RX   PubMed=11058132; DOI=10.1093/nar/28.21.4317;
RA   Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F.,
RA   Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.;
RT   "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans
RT   and genomic sequence comparison with Bacillus subtilis.";
RL   Nucleic Acids Res. 28:4317-4331(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-330.
RC   STRAIN=ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125;
RX   PubMed=10484179; DOI=10.1007/s007920050120;
RA   Takami H., Takaki Y., Nakasone K., Sakiyama T., Maeno G., Sasaki R.,
RA   Hirama C., Fuji F., Masui N.;
RT   "Genetic analysis of the chromosome of alkaliphilic Bacillus halodurans C-
RT   125.";
RL   Extremophiles 3:227-233(1999).
CC   -!- FUNCTION: Required for spore cortex hydrolysis during germination.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SleB family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA83916.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BA000004; BAB05350.1; -; Genomic_DNA.
DR   EMBL; AB024552; BAA83916.1; ALT_INIT; Genomic_DNA.
DR   PIR; G83853; G83853.
DR   RefSeq; WP_010897794.1; NC_002570.2.
DR   AlphaFoldDB; Q9KCE0; -.
DR   SMR; Q9KCE0; -.
DR   STRING; 272558.10174248; -.
DR   EnsemblBacteria; BAB05350; BAB05350; BAB05350.
DR   KEGG; bha:BH1631; -.
DR   eggNOG; COG3409; Bacteria.
DR   eggNOG; COG3773; Bacteria.
DR   HOGENOM; CLU_053345_0_0_9; -.
DR   OMA; WAVRNYQ; -.
DR   OrthoDB; 682655at2; -.
DR   Proteomes; UP000001258; Chromosome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009847; P:spore germination; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.2520; -; 1.
DR   Gene3D; 1.10.101.10; -; 1.
DR   InterPro; IPR011105; Cell_wall_hydrolase_SleB.
DR   InterPro; IPR002477; Peptidoglycan-bd-like.
DR   InterPro; IPR036365; PGBD-like_sf.
DR   InterPro; IPR036366; PGBDSf.
DR   InterPro; IPR042047; SleB_dom1.
DR   InterPro; IPR014224; Spore_cortex_SleB.
DR   Pfam; PF07486; Hydrolase_2; 1.
DR   Pfam; PF01471; PG_binding_1; 1.
DR   SUPFAM; SSF47090; SSF47090; 1.
DR   TIGRFAMs; TIGR02869; spore_SleB; 1.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Germination; Hydrolase;
KW   Reference proteome; Signal; Sporulation.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..330
FT                   /note="Spore cortex-lytic enzyme"
FT                   /id="PRO_0000022357"
FT   REGION          117..195
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        131..175
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        176..190
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   330 AA;  36565 MW;  790413286D28C51D CRC64;
     MSTRGWLKIP LLSLALFLSL MSMTTTAHAF SDQVIQHGAT GDDVVELQAR LQYIGFYNKK
     IDGVFGWSTY WAVRNYQYEF GMEVDGLVGP EMKAKLEKTT NFNRDFVDRA LTQGRKFTHY
     GGVPKQIQKG PKGSADERRR GREGVRQPRA DRPGRDAPAA PRDERAPRRE ERPAPTPEPT
     PAPPEEPTPY EEAPDAQDDE ANIEKATNVP AGYSDNDIQL MAQAVYGEAR GEPYVGQVAV
     AAVILNRLNS PTFPDNVSGV IFEPLAFTAV ADGQIYMTPD ETARRAVLDA LNGQDPSGGA
     TYYFNPDTAT SGWIWSRPQI KRIGKHIFCN