ID   SLEB_BACSU              Reviewed;         305 AA.
AC   P50739;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Spore cortex-lytic enzyme;
DE            Short=SCLE;
DE   Flags: Precursor;
GN   Name=sleB; Synonyms=ypeA; OrderedLocusNames=BSU22930;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8830707; DOI=10.1128/jb.178.20.6059-6063.1996;
RA   Moriyama R., Hattori A., Miyata S., Kudoh S., Makino S.;
RT   "A gene (sleB) encoding a spore cortex-lytic enzyme from Bacillus subtilis
RT   and response of the enzyme to L-alanine-mediated germination.";
RL   J. Bacteriol. 178:6059-6063(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / NRRL NRS-744 / VKM B-501;
RX   PubMed=8760912; DOI=10.1099/13500872-142-8-2005;
RA   Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D.,
RA   Serror P.;
RT   "Sequence analysis of the Bacillus subtilis chromosome region between the
RT   serA and kdg loci cloned in a yeast artificial chromosome.";
RL   Microbiology 142:2005-2016(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   CHARACTERIZATION.
RC   STRAIN=168;
RX   PubMed=10197998; DOI=10.1128/jb.181.8.2373-2378.1999;
RA   Moriyama R., Fukuoka H., Miyata S., Kudoh S., Hattori A., Kozuka S.,
RA   Yasuda Y., Tochikubo K., Makino S.;
RT   "Expression of a germination-specific amidase, SleB, of Bacilli in the
RT   forespore compartment of sporulating cells and its localization on the
RT   exterior side of the cortex in dormant spores.";
RL   J. Bacteriol. 181:2373-2378(1999).
RN   [5]
RP   CHARACTERIZATION.
RC   STRAIN=168;
RX   PubMed=10658652; DOI=10.1099/00221287-146-1-57;
RA   Boland F.M., Atrih A., Chirakkal H., Foster S.J., Moir A.;
RT   "Complete spore-cortex hydrolysis during germination of Bacillus subtilis
RT   168 requires SleB and YpeB.";
RL   Microbiology 146:57-64(2000).
CC   -!- FUNCTION: Could be a lytic transglycosylase. Required for spore cortex
CC       hydrolysis during germination. Interacts strongly but noncovalently
CC       with spore components.
CC   -!- SUBCELLULAR LOCATION: Forespore. Note=Expressed in the forespore and
CC       then transported across the inner forespore membrane and deposited on
CC       the outside of the cortex.
CC   -!- DEVELOPMENTAL STAGE: Expressed during sporulation and active during
CC       germination. Exists as mature but inactive form in the dormant spore.
CC   -!- INDUCTION: Expression is sigma G-dependent.
CC   -!- MISCELLANEOUS: B.subtilis SleB could not be detected with anti-B.cereus
CC       SleB antiserum and vice versa.
CC   -!- SIMILARITY: Belongs to the SleB family. {ECO:0000305}.
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DR   EMBL; D79978; BAA11473.1; -; Genomic_DNA.
DR   EMBL; L47648; AAC83957.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14209.1; -; Genomic_DNA.
DR   PIR; C69708; C69708.
DR   RefSeq; NP_390174.1; NC_000964.3.
DR   RefSeq; WP_004398523.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; P50739; -.
DR   SMR; P50739; -.
DR   STRING; 224308.BSU22930; -.
DR   PaxDb; P50739; -.
DR   PRIDE; P50739; -.
DR   DNASU; 938979; -.
DR   EnsemblBacteria; CAB14209; CAB14209; BSU_22930.
DR   GeneID; 938979; -.
DR   KEGG; bsu:BSU22930; -.
DR   PATRIC; fig|224308.179.peg.2500; -.
DR   eggNOG; COG3409; Bacteria.
DR   eggNOG; COG3773; Bacteria.
DR   OMA; KTYMAVR; -.
DR   BioCyc; BSUB:BSU22930-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0042763; C:intracellular immature spore; IEA:UniProtKB-SubCell.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009847; P:spore germination; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.2520; -; 1.
DR   Gene3D; 1.10.101.10; -; 1.
DR   InterPro; IPR011105; Cell_wall_hydrolase_SleB.
DR   InterPro; IPR002477; Peptidoglycan-bd-like.
DR   InterPro; IPR036365; PGBD-like_sf.
DR   InterPro; IPR036366; PGBDSf.
DR   InterPro; IPR042047; SleB_dom1.
DR   InterPro; IPR014224; Spore_cortex_SleB.
DR   Pfam; PF07486; Hydrolase_2; 1.
DR   Pfam; PF01471; PG_binding_1; 1.
DR   SUPFAM; SSF47090; SSF47090; 1.
DR   TIGRFAMs; TIGR02869; spore_SleB; 1.
PE   1: Evidence at protein level;
KW   Cell wall biogenesis/degradation; Germination; Hydrolase;
KW   Reference proteome; Signal; Sporulation.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..305
FT                   /note="Spore cortex-lytic enzyme"
FT                   /id="PRO_0000022358"
FT   REGION          130..185
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        131..145
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        155..174
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   305 AA;  34002 MW;  9DF1305975F5BE16 CRC64;
     MKSKGSIMAC LILFSFTITT FINTETISAF SNQVIQRGAT GDDVVELQAR LQYNGYYNGK
     IDGVYGWGTY WAVRNFQDQF GLKEVDGLVG AKTKQTLICK SKYYREYVME QLNKGNTFTH
     YGKIPLKYQT KPSKAATQKA RQQAEARQKQ PAEKTTQKPK ANANKQQNNT PAKARKQDAV
     AANMPGGFSN NDIRLLAQAV YGEARGEPYE GQVAIAAVIL NRLNSPLFPN SVAGVIFEPL
     AFTAVADGQI YMQPNETARE AVLDAINGWD PSEEALYYFN PDTATSPWIW GRPQIKRIGK
     HIFCE