BI1_USTMA
ID BI1_USTMA Reviewed; 410 AA.
AC Q0H8X2;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Probable intron-encoded endonuclease bI1;
DE Contains:
DE RecName: Full=Truncated non-functional cytochrome b;
DE Contains:
DE RecName: Full=Intron-encoded endonuclease bI1;
DE EC=3.1.-.-;
DE Flags: Precursor;
GN Name=bI1;
OS Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OG Mitochondrion.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=237631;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RA Kennell J.C., Boehmer C.;
RT "Annotation of mitochondrial genome of Ustilago maydis and comparative
RT analysis of basidiomycete mtDNAs.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=17080091; DOI=10.1038/nature05248;
RA Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA Birren B.W.;
RT "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT maydis.";
RL Nature 444:97-101(2006).
CC -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion. Membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- PTM: The mature protein may arise from proteolytic cleavage of an in-
CC frame translation of COB exon 1 plus intron 1, containing the bI1 open
CC reading frame.
CC -!- SIMILARITY: To endonucleases of group I introns of fungi and phage.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAZ67030.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; DQ157700; AAZ67030.1; ALT_INIT; Genomic_DNA.
DR EMBL; AACP01000277; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; YP_762700.1; NC_008368.1.
DR AlphaFoldDB; Q0H8X2; -.
DR SMR; Q0H8X2; -.
DR STRING; 237631.Q0H8X2; -.
DR PRIDE; Q0H8X2; -.
DR GeneID; 4308280; -.
DR InParanoid; Q0H8X2; -.
DR Proteomes; UP000000561; Mitochondrion.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR CDD; cd00284; Cytochrome_b_N; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.20.810.10; -; 1.
DR Gene3D; 3.40.1440.10; -; 1.
DR InterPro; IPR005797; Cyt_b/b6_N.
DR InterPro; IPR027387; Cytb/b6-like_sf.
DR InterPro; IPR016174; Di-haem_cyt_TM.
DR InterPro; IPR000305; GIY-YIG_endonuc.
DR InterPro; IPR035901; GIY-YIG_endonuc_sf.
DR InterPro; IPR006350; Intron_endoG1.
DR InterPro; IPR003647; Intron_nuc_1_rpt.
DR InterPro; IPR010896; NUMOD1.
DR InterPro; IPR003611; NUMOD3.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00033; Cytochrome_B; 1.
DR Pfam; PF01541; GIY-YIG; 1.
DR Pfam; PF07453; NUMOD1; 1.
DR Pfam; PF07460; NUMOD3; 1.
DR SMART; SM00465; GIYc; 1.
DR SMART; SM00497; IENR1; 1.
DR SMART; SM00496; IENR2; 2.
DR SUPFAM; SSF81342; SSF81342; 1.
DR SUPFAM; SSF82771; SSF82771; 1.
DR TIGRFAMs; TIGR01453; grpIintron_endo; 1.
DR PROSITE; PS51002; CYTB_NTER; 1.
DR PROSITE; PS50164; GIY_YIG; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Intron homing; Membrane; Mitochondrion; Nuclease;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..?
FT /note="Truncated non-functional cytochrome b"
FT /id="PRO_0000271164"
FT CHAIN ?..410
FT /note="Intron-encoded endonuclease bI1"
FT /id="PRO_0000271165"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00968"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00968"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00968"
FT DOMAIN 196..286
FT /note="GIY-YIG"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00977"
FT REGION 1..131
FT /note="COB exon 1 encoded"
FT REGION 132..410
FT /note="COB intron 1 encoded"
SQ SEQUENCE 410 AA; 46822 MW; 57A0A58B7BC913E5 CRC64;
MRLLKTHPIL GLANSYLIDS PQPSNISYMW NFGSLLGVCL IIQILTGVFL AMHYTPSVDL
AFISVEHIMR DVNYGWLIRY LHANTASFFF IFVYLHIGRG LYYGSYKSPR TLLWSIGVII
LVLMMAIAFL GFNGQKYMCF YNIDITIIQY LSIPTLITPS TRLKPILDKH NIKPVLLFEN
LTNSETKKIA YQALKPFSGI YMIVNLITEK YYVGSAVTGN LYMRFHKHLF SFTGNKRVAN
AVNKYGLSEF AFLVLEIVPQ KDKIDSTLLL NREDYYLETL KPEYNIAPLA SNSLGWKHSE
ESLAKMRENY SEERRQQVAN INKGKTLSEE TRELIRKSAL LRKSMSSETR MKCAVNVQPV
TIINLDGTNI MNFVSIKEAS IAISCNEKTI RRALNGNGIV KKNYIVKVIK
