SLFN5_HUMAN
ID SLFN5_HUMAN Reviewed; 891 AA.
AC Q08AF3; Q08AF2; Q8WU54; Q96A82;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Schlafen family member 5;
GN Name=SLFN5;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-782 (ISOFORM 1), AND VARIANT
RP LEU-787.
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [4]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-59, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- FUNCTION: May have a role in hematopoietic cell differentiation.
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q08AF3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q08AF3-2; Sequence=VSP_024271, VSP_024272;
CC -!- SIMILARITY: Belongs to the Schlafen family. Subgroup III subfamily.
CC {ECO:0000305}.
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DR EMBL; BC021238; AAH21238.1; -; mRNA.
DR EMBL; BC125200; AAI25201.1; -; mRNA.
DR EMBL; BC125201; AAI25202.1; -; mRNA.
DR EMBL; AK054668; BAB70788.1; -; mRNA.
DR CCDS; CCDS32619.1; -. [Q08AF3-1]
DR RefSeq; NP_659412.3; NM_144975.3. [Q08AF3-1]
DR PDB; 6RR9; X-ray; 3.43 A; A=1-336.
DR PDB; 7CUX; X-ray; 3.29 A; A/B/C/D/E/F=12-337.
DR PDB; 7PPJ; EM; 3.44 A; A=1-891.
DR PDB; 7Q3Z; X-ray; 1.85 A; A/B=1-336.
DR PDBsum; 6RR9; -.
DR PDBsum; 7CUX; -.
DR PDBsum; 7PPJ; -.
DR PDBsum; 7Q3Z; -.
DR AlphaFoldDB; Q08AF3; -.
DR SMR; Q08AF3; -.
DR BioGRID; 127815; 67.
DR IntAct; Q08AF3; 9.
DR MINT; Q08AF3; -.
DR STRING; 9606.ENSP00000299977; -.
DR GlyGen; Q08AF3; 2 sites, 2 O-linked glycans (2 sites).
DR iPTMnet; Q08AF3; -.
DR PhosphoSitePlus; Q08AF3; -.
DR SwissPalm; Q08AF3; -.
DR BioMuta; SLFN5; -.
DR DMDM; 121939987; -.
DR EPD; Q08AF3; -.
DR jPOST; Q08AF3; -.
DR MassIVE; Q08AF3; -.
DR MaxQB; Q08AF3; -.
DR PaxDb; Q08AF3; -.
DR PeptideAtlas; Q08AF3; -.
DR PRIDE; Q08AF3; -.
DR ProteomicsDB; 58663; -. [Q08AF3-1]
DR ProteomicsDB; 58664; -. [Q08AF3-2]
DR Antibodypedia; 2910; 26 antibodies from 15 providers.
DR DNASU; 162394; -.
DR Ensembl; ENST00000299977.9; ENSP00000299977.3; ENSG00000166750.10. [Q08AF3-1]
DR Ensembl; ENST00000592325.1; ENSP00000466984.1; ENSG00000166750.10. [Q08AF3-2]
DR GeneID; 162394; -.
DR KEGG; hsa:162394; -.
DR MANE-Select; ENST00000299977.9; ENSP00000299977.3; NM_144975.4; NP_659412.3.
DR UCSC; uc002hje.4; human. [Q08AF3-1]
DR CTD; 162394; -.
DR DisGeNET; 162394; -.
DR GeneCards; SLFN5; -.
DR HGNC; HGNC:28286; SLFN5.
DR HPA; ENSG00000166750; Low tissue specificity.
DR MIM; 614952; gene.
DR neXtProt; NX_Q08AF3; -.
DR OpenTargets; ENSG00000166750; -.
DR PharmGKB; PA144596357; -.
DR VEuPathDB; HostDB:ENSG00000166750; -.
DR eggNOG; ENOG502QWKG; Eukaryota.
DR GeneTree; ENSGT00410000025651; -.
DR HOGENOM; CLU_007071_0_0_1; -.
DR InParanoid; Q08AF3; -.
DR OMA; NGYSPRD; -.
DR OrthoDB; 211385at2759; -.
DR PhylomeDB; Q08AF3; -.
DR TreeFam; TF337168; -.
DR PathwayCommons; Q08AF3; -.
DR SignaLink; Q08AF3; -.
DR BioGRID-ORCS; 162394; 11 hits in 1075 CRISPR screens.
DR ChiTaRS; SLFN5; human.
DR GenomeRNAi; 162394; -.
DR Pharos; Q08AF3; Tbio.
DR PRO; PR:Q08AF3; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q08AF3; protein.
DR Bgee; ENSG00000166750; Expressed in visceral pleura and 188 other tissues.
DR ExpressionAtlas; Q08AF3; baseline and differential.
DR Genevisible; Q08AF3; HS.
DR GO; GO:0005634; C:nucleus; IEA:Ensembl.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR Gene3D; 3.30.950.30; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR029684; Schlafen.
DR InterPro; IPR007421; Schlafen_AlbA_2_dom.
DR InterPro; IPR038461; Schlafen_AlbA_2_dom_sf.
DR InterPro; IPR029671; Slfn5.
DR InterPro; IPR018647; SLFN_3-like_DNA/RNA_helicase.
DR PANTHER; PTHR12155; PTHR12155; 1.
DR PANTHER; PTHR12155:SF26; PTHR12155:SF26; 1.
DR Pfam; PF04326; AlbA_2; 1.
DR Pfam; PF09848; DUF2075; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; ATP-binding; Differentiation;
KW Isopeptide bond; Nucleotide-binding; Reference proteome; Ubl conjugation.
FT CHAIN 1..891
FT /note="Schlafen family member 5"
FT /id="PRO_0000282982"
FT BINDING 578..585
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CROSSLNK 59
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VAR_SEQ 338
FT /note="D -> G (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_024271"
FT VAR_SEQ 339..891
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_024272"
FT VARIANT 754
FT /note="V -> L (in dbSNP:rs16970806)"
FT /id="VAR_053876"
FT VARIANT 787
FT /note="P -> L (in dbSNP:rs11651240)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_031445"
FT VARIANT 797
FT /note="A -> V (in dbSNP:rs2291189)"
FT /id="VAR_031446"
FT CONFLICT 183
FT /note="Y -> H (in Ref. 1; AAI25202)"
FT /evidence="ECO:0000305"
FT STRAND 6..8
FT /evidence="ECO:0007829|PDB:6RR9"
FT STRAND 9..13
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 15..21
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT HELIX 25..30
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT HELIX 33..51
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT TURN 52..54
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 56..62
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT HELIX 69..72
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT HELIX 80..83
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 86..91
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 94..99
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT HELIX 105..107
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 118..121
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 124..127
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT HELIX 130..140
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT HELIX 170..176
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 179..182
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 195..198
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT HELIX 204..221
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 225..229
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT TURN 233..236
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT HELIX 243..245
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT HELIX 248..261
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 268..270
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 276..285
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 288..298
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 302..305
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 307..309
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 313..316
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 319..322
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT HELIX 325..332
FT /evidence="ECO:0007829|PDB:7Q3Z"
FT STRAND 345..349
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 361..367
FT /evidence="ECO:0007829|PDB:7PPJ"
FT HELIX 370..377
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 384..388
FT /evidence="ECO:0007829|PDB:7PPJ"
FT HELIX 390..399
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 400..402
FT /evidence="ECO:0007829|PDB:7PPJ"
FT HELIX 403..410
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 416..423
FT /evidence="ECO:0007829|PDB:7PPJ"
FT HELIX 425..429
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 435..444
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 451..458
FT /evidence="ECO:0007829|PDB:7PPJ"
FT HELIX 463..479
FT /evidence="ECO:0007829|PDB:7PPJ"
FT TURN 480..482
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 491..496
FT /evidence="ECO:0007829|PDB:7PPJ"
FT TURN 516..518
FT /evidence="ECO:0007829|PDB:7PPJ"
FT TURN 523..525
FT /evidence="ECO:0007829|PDB:7PPJ"
FT HELIX 526..538
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 539..541
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 543..545
FT /evidence="ECO:0007829|PDB:7PPJ"
FT TURN 546..548
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 553..555
FT /evidence="ECO:0007829|PDB:7PPJ"
FT HELIX 558..565
FT /evidence="ECO:0007829|PDB:7PPJ"
FT TURN 568..570
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 572..575
FT /evidence="ECO:0007829|PDB:7PPJ"
FT HELIX 583..598
FT /evidence="ECO:0007829|PDB:7PPJ"
FT HELIX 603..605
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 606..609
FT /evidence="ECO:0007829|PDB:7PPJ"
FT HELIX 613..620
FT /evidence="ECO:0007829|PDB:7PPJ"
FT HELIX 632..636
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 645..649
FT /evidence="ECO:0007829|PDB:7PPJ"
FT HELIX 651..653
FT /evidence="ECO:0007829|PDB:7PPJ"
FT TURN 656..658
FT /evidence="ECO:0007829|PDB:7PPJ"
FT HELIX 661..670
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 673..675
FT /evidence="ECO:0007829|PDB:7PPJ"
FT STRAND 679..682
FT /evidence="ECO:0007829|PDB:7PPJ"
SQ SEQUENCE 891 AA; 101055 MW; 52F2AC3259251AB5 CRC64;
MSLRIDVDTN FPECVVDAGK VTLGTQQRQE MDPRLREKQN EIILRAVCAL LNSGGGIIKA
EIENKGYNYE RHGVGLDVPP IFRSHLDKMQ KENHFLIFVK SWNTEAGVPL ATLCSNLYHR
ERTSTDVMDS QEALAFLKCR TQTPTNINVS NSLGPQAAQG SVQYEGNINV SAAALFDRKR
LQYLEKLNLP ESTHVEFVMF STDVSHCVKD RLPKCVSAFA NTEGGYVFFG VHDETCQVIG
CEKEKIDLTS LRASIDGCIK KLPVHHFCTQ RPEIKYVLNF LEVHDKGALR GYVCAIKVEK
FCCAVFAKVP SSWQVKDNRV RQLPTREWTA WMMEADPDLS RCPEMVLQLS LSSATPRSKP
VCIHKNSECL KEQQKRYFPV FSDRVVYTPE SLYKELFSQH KGLRDLINTE MRPFSQGILI
FSQSWAVDLG LQEKQGVICD ALLISQNNTP ILYTIFSKWD AGCKGYSMIV AYSLKQKLVN
KGGYTGRLCI TPLVCVLNSD RKAQSVYSSY LQIYPESYNF MTPQHMEALL QSLVIVLLGF
KSFLSEELGS EVLNLLTNKQ YELLSKNLRK TRELFVHGLP GSGKTILALR IMEKIRNVFH
CEPANILYIC ENQPLKKLVS FSKKNICQPV TRKTFMKNNF EHIQHIIIDD AQNFRTEDGD
WYGKAKFITQ TARDGPGVLW IFLDYFQTYH LSCSGLPPPS DQYPREEINR VVRNAGPIAN
YLQQVMQEAR QNPPPNLPPG SLVMLYEPKW AQGVPGNLEI IEDLNLEEIL IYVANKCRFL
LRNGYSPKDI AVLFTKASEV EKYKDRLLTA MRKRKLSQLH EESDLLLQIG DASDVLTDHI
VLDSVCRFSG LERNIVFGIN PGVAPPAGAY NLLLCLASRA KRHLYILKAS V
