SLI15_YEAST
ID SLI15_YEAST Reviewed; 698 AA.
AC P38283; D6VQF1;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Inner centromere protein-related protein SLI15;
DE Short=INCENP-related protein SLI15;
GN Name=SLI15; OrderedLocusNames=YBR156C; ORFNames=YBR1206;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8488729; DOI=10.1002/yea.320090308;
RA Baur A., Schaaff-Gerstenschlaeger I., Boles E., Miosga T., Rose M.,
RA Zimmermann F.K.;
RT "Sequence of a 4.8 kb fragment of Saccharomyces cerevisiae chromosome II
RT including three essential open reading frames.";
RL Yeast 9:289-293(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7483850; DOI=10.1002/yea.320110908;
RA Rose M., Kiesau P., Proft M., Entian K.-D.;
RT "Sequence and functional analysis of a 7.2 kb DNA fragment containing four
RT open reading frames located between RPB5 and CDC28 on the right arm of
RT chromosome II.";
RL Yeast 11:865-871(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=10385519; DOI=10.1083/jcb.145.7.1381;
RA Kim J.-H., Kang J.-S., Chan C.S.M.;
RT "Sli15 associates with the ipl1 protein kinase to promote proper chromosome
RT segregation in Saccharomyces cerevisiae.";
RL J. Cell Biol. 145:1381-1394(1999).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND PHOSPHORYLATION.
RX PubMed=11724818; DOI=10.1083/jcb.200105029;
RA Kang J.-S., Cheeseman I.M., Kallstrom G., Velmurugan S., Barnes G.,
RA Chan C.S.M.;
RT "Functional cooperation of Dam1, Ipl1, and the inner centromere protein
RT (INCENP)-related protein Sli15 during chromosome segregation.";
RL J. Cell Biol. 155:763-774(2001).
RN [7]
RP FUNCTION.
RX PubMed=11853667; DOI=10.1016/s0092-8674(02)00633-5;
RA Tanaka T.U., Rachidi N., Janke C., Pereira G., Galova M., Schiebel E.,
RA Stark M.J.R., Nasmyth K.;
RT "Evidence that the Ipl1-Sli15 (Aurora kinase-INCENP) complex promotes
RT chromosome bi-orientation by altering kinetochore-spindle pole
RT connections.";
RL Cell 108:317-329(2002).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT "Analysis of phosphorylation sites on proteins from Saccharomyces
RT cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-489, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [11]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-489, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Required for high-fidelity chromosome segregation during the
CC later part of each cell cycle in association with the protein kinase
CC IPL1. Stimulates IPL1 kinase activity and facilitates its association
CC with the mitotic spindle. Has a role in attaching the kinetochores to
CC the microtubules and ensuring that sister kinetochores connect to
CC opposite poles. {ECO:0000269|PubMed:10385519,
CC ECO:0000269|PubMed:11724818, ECO:0000269|PubMed:11853667}.
CC -!- INTERACTION:
CC P38283; P47134: BIR1; NbExp=8; IntAct=EBI-20842, EBI-3648;
CC P38283; Q00684: CDC14; NbExp=2; IntAct=EBI-20842, EBI-4192;
CC P38283; P24869: CLB2; NbExp=2; IntAct=EBI-20842, EBI-4515;
CC P38283; P53267: DAM1; NbExp=2; IntAct=EBI-20842, EBI-23268;
CC P38283; P38991: IPL1; NbExp=10; IntAct=EBI-20842, EBI-9319;
CC P38283; Q3E7Y6: NBL1; NbExp=5; IntAct=EBI-20842, EBI-9513736;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10385519}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:10385519,
CC ECO:0000269|PubMed:11724818}. Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:11724818}. Note=Associates with the mitotic spindle
CC and the kinetochore (PubMed:11724818). {ECO:0000269|PubMed:11724818}.
CC -!- PTM: Phosphorylated by serine/threonine protein kinase IPL1.
CC {ECO:0000269|PubMed:11724818}.
CC -!- MISCELLANEOUS: Present with 319 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the INCENP family. {ECO:0000305}.
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DR EMBL; X71329; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; S59774; AAC60557.1; -; Genomic_DNA.
DR EMBL; Z36025; CAA85115.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07271.1; -; Genomic_DNA.
DR PIR; S46027; S46027.
DR RefSeq; NP_009714.3; NM_001178504.3.
DR AlphaFoldDB; P38283; -.
DR SMR; P38283; -.
DR BioGRID; 32855; 342.
DR ComplexPortal; CPX-1900; Chromosomal passenger complex.
DR DIP; DIP-5807N; -.
DR IntAct; P38283; 7.
DR MINT; P38283; -.
DR STRING; 4932.YBR156C; -.
DR iPTMnet; P38283; -.
DR MaxQB; P38283; -.
DR PaxDb; P38283; -.
DR PRIDE; P38283; -.
DR EnsemblFungi; YBR156C_mRNA; YBR156C; YBR156C.
DR GeneID; 852453; -.
DR KEGG; sce:YBR156C; -.
DR SGD; S000000360; SLI15.
DR VEuPathDB; FungiDB:YBR156C; -.
DR eggNOG; ENOG502S0AD; Eukaryota.
DR HOGENOM; CLU_015675_0_0_1; -.
DR InParanoid; P38283; -.
DR OMA; RSNMFVP; -.
DR BioCyc; YEAST:G3O-29106-MON; -.
DR PRO; PR:P38283; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38283; protein.
DR GO; GO:0032133; C:chromosome passenger complex; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0005828; C:kinetochore microtubule; IDA:SGD.
DR GO; GO:0072686; C:mitotic spindle; IDA:CACAO.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005876; C:spindle microtubule; IDA:SGD.
DR GO; GO:0051233; C:spindle midzone; IDA:SGD.
DR GO; GO:0030295; F:protein kinase activator activity; IMP:SGD.
DR GO; GO:0032147; P:activation of protein kinase activity; IDA:CACAO.
DR GO; GO:0051316; P:attachment of spindle microtubules to kinetochore involved in meiotic chromosome segregation; IC:ComplexPortal.
DR GO; GO:0007059; P:chromosome segregation; IMP:SGD.
DR GO; GO:0090267; P:positive regulation of mitotic cell cycle spindle assembly checkpoint; IC:ComplexPortal.
DR GO; GO:0006468; P:protein phosphorylation; IDA:SGD.
DR GO; GO:0032465; P:regulation of cytokinesis; IMP:SGD.
DR GO; GO:0031134; P:sister chromatid biorientation; IC:ComplexPortal.
DR InterPro; IPR005635; Inner_centromere_prot_ARK-bd.
DR Pfam; PF03941; INCENP_ARK-bind; 1.
PE 1: Evidence at protein level;
KW Centromere; Chromosome; Chromosome partition; Cytoplasm; Cytoskeleton;
KW Kinetochore; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..698
FT /note="Inner centromere protein-related protein SLI15"
FT /id="PRO_0000071956"
FT REGION 365..390
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 405..444
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 455..474
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 535..560
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 365..380
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 405..419
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 420..444
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 459..474
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 268
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17287358"
FT MOD_RES 489
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
SQ SEQUENCE 698 AA; 79186 MW; 827AC2AB884BA7D1 CRC64;
MDWAIKAARK KTQRKPGSTR SIIETLDDLN NLTTDAHSEI NQRLYESSEW LRNNVYMNTL
KYEDKKMEES LISPENTHNK MDVEFPKMKG EYELSNSQND AAKDVTKTPR NGLHNDKSIT
PKSLRRKEVT EGMNRFSIHD TNKSPVEPLN SVKVDANESE KSSPWSPYKV EKVLRESSKT
SESPINTKRF DNQTWAAKEE MENEPILQAL KKAESVKVKP PPNSGIARSQ RRSNMFVPLP
NKDPLIIQHI PPTKSSGSIP KVRTVKESPI AFKKKSTINS PAIRAVENSD TAGSTKASSV
FDRLSSIPTK SFENKISRGN VGHKYSSSSI DLTGSPMKKV SQKFKSINST DTDMQEALRD
IFSVKNKITK NNSPKGKNSR KSSIPRFDKT SLKLTTHKKL AIIAEQKKKS KHSSDVHKTG
SRPHSISPTK ISVDSSSPSK EVKNYYQSPV RGYLRPTKAS ISPNKNKNLT TSQTPHRLKI
KEKTLRKLSP NIADISKPES RKSKNYRLTN LQLLPPAEAE RDDLKKKFDK RLSGIMRSQQ
EHHRRKQEKQ KRMSHLEQDL KKQTSFSNDY KDIRLKESLA PFDNHVRDTI NKNTAFSTDN
ILATINTVDH REIIGNVTPK IASVNDSLPE INTDSEDEAS VTLAAWAKSP YLQEQLIRQQ
DINPQTIFGP IPPLHTDEIF PNPRLNRLKP RQIVPKRS