SLIP_MOUSE
ID SLIP_MOUSE Reviewed; 796 AA.
AC D3YWJ0;
DT 11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Nuclear GTPase SLIP-GC {ECO:0000250|UniProtKB:Q68CJ6};
DE EC=3.6.1.- {ECO:0000305};
DE AltName: Full=Speckled-like pattern in the germinal center {ECO:0000250|UniProtKB:Q68CJ6};
GN Name=Nuggc {ECO:0000312|MGI:MGI:2685446};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|Proteomes:UP000000589};
RN [1] {ECO:0000312|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=22833677; DOI=10.1074/jbc.m112.340661;
RA Richter K., Burch L., Chao F., Henke D., Jiang C., Daly J., Zhao M.L.,
RA Kissling G., Diaz M.;
RT "Altered pattern of immunoglobulin hypermutation in mice deficient in Slip-
RT GC protein.";
RL J. Biol. Chem. 287:31856-31865(2012).
CC -!- FUNCTION: Nuclear GTPase found in germinal center B-cells, where it may
CC inhibit function of the activation-induced cytidine deaminase AICDA (By
CC similarity). Reduces somatic hypermutation in B-cells which may enhance
CC genome stability (PubMed:22833677). {ECO:0000250|UniProtKB:Q68CJ6,
CC ECO:0000269|PubMed:22833677}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000269|PubMed:22833677}.
CC -!- DISRUPTION PHENOTYPE: B-cell and T-cell development is unaffected, and
CC germinal centers form normally. Class-switch recombination in B-cells
CC is not affected. B-cells in older mice (3 months onwards) show
CC significantly increased somatic hypermutation near the immunoglobulin
CC heavy chain locus, mainly at G:C base pairs. Other loci also show
CC increased rates of somatic hypermutation.
CC {ECO:0000269|PubMed:22833677}.
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DR EMBL; AC122274; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_006518364.1; XM_006518301.2.
DR RefSeq; XP_006518365.1; XM_006518302.1.
DR RefSeq; XP_006518366.1; XM_006518303.2.
DR AlphaFoldDB; D3YWJ0; -.
DR STRING; 10090.ENSMUSP00000078434; -.
DR iPTMnet; D3YWJ0; -.
DR PhosphoSitePlus; D3YWJ0; -.
DR PaxDb; D3YWJ0; -.
DR PeptideAtlas; D3YWJ0; -.
DR PRIDE; D3YWJ0; -.
DR Antibodypedia; 5386; 36 antibodies from 13 providers.
DR Ensembl; ENSMUST00000150897; ENSMUSP00000118402; ENSMUSG00000061356.
DR GeneID; 100503545; -.
DR CTD; 389643; -.
DR MGI; MGI:2685446; Nuggc.
DR VEuPathDB; HostDB:ENSMUSG00000061356; -.
DR eggNOG; ENOG502QVUX; Eukaryota.
DR GeneTree; ENSGT00390000007091; -.
DR HOGENOM; CLU_019456_0_0_1; -.
DR PhylomeDB; D3YWJ0; -.
DR BioGRID-ORCS; 100503545; 3 hits in 108 CRISPR screens.
DR ChiTaRS; Nuggc; mouse.
DR PRO; PR:D3YWJ0; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; D3YWJ0; protein.
DR Bgee; ENSMUSG00000061356; Expressed in spermatocyte and 13 other tissues.
DR ExpressionAtlas; D3YWJ0; baseline and differential.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; ISO:MGI.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IMP:MGI.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; ISO:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:MGI.
DR GO; GO:0033262; P:regulation of nuclear cell cycle DNA replication; ISO:MGI.
DR GO; GO:0016446; P:somatic hypermutation of immunoglobulin genes; IMP:MGI.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR045063; Dynamin_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00350; Dynamin_N; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 3: Inferred from homology;
KW Coiled coil; GTP-binding; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..796
FT /note="Nuclear GTPase SLIP-GC"
FT /id="PRO_0000436145"
FT COILED 158..185
FT /evidence="ECO:0000255"
FT COILED 742..776
FT /evidence="ECO:0000255"
FT BINDING 107..114
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00499"
SQ SEQUENCE 796 AA; 91269 MW; 0188246FA7D4C78F CRC64;
MEEMEALVGV VPHSADCDLF KEPVRKRRRL HRDRQFQAFP SAEQSALKEY EKLECRTRRV
LSNTYQKLIQ SVFLDDSIPS GLKYLINRLL ALIEKSPLEP VYVGFLGITG AGKSSLINAL
IRQAMFLPVS GESVCTSCIV QVSSGCCEQY EAKIHLLSDQ EWKAELKDLT KLLHRAEQSG
EEEADLWDRD DATEEAAQKL RMLYGHGAER RHYEELLRLK PRGRIPNSRT ITLKAEEAGE
LSVKLDPYIR TRRRDWDGES AETQIWPLIK YVEVILPKSA LIPEGVVLVD IPGTGDFNSK
RDKMWKKTID KCSVIWVISD IERVSGGKTH EDLLSESIKA CQRGFCRDIA LVVTKTDKLH
LQEYLRERKM GNQAIQSQRE AVLQRNEIIK LQRKRMLKEK LKRKLPADSK VLEASDLVYT
VSAHEYWQRT ILTEEESEIP KLREYIRKRI LDKKRRLVTK YVTEAFGLLL LTDTLNTEES
LLTEELNTGG LRQFVEEKME LLEKAIEQCF ARMEQPLQTG VQVAMTSYRR ILGSCLVRSR
GNQGFHQTLK AVCLKNGVYA SRTLARIDLN EALSQPIYDQ IDPVFGGIFR DGKPTAPALM
QHIDAFKHSL EERMAEVGVR SGWKQDGYKR SFLIQEISAI LGGLESHILR RKRKIYKSVT
SSIQNDLKPC YEEAAQITGK KACERMKDVI RRGVERQVAE GLFERAQERM WHQFRQLKHG
ITEKVKGSIT TMLTLAAPQG VGLCKELADV RNEQKEMEKL YRSLREVAEN AQLRRSMQDF
LLRMSPSKAG PHGTKL
