SLK19_YEAST
ID SLK19_YEAST Reviewed; 821 AA.
AC Q08581; D6W2Q4;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Kinetochore protein SLK19;
DE AltName: Full=Synthetic lethal KAR3 protein 19;
GN Name=SLK19; OrderedLocusNames=YOR195W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=10427094; DOI=10.1083/jcb.146.2.415;
RA Zeng X., Kahana J.A., Silver P.A., Morphew M.K., McIntosh J.R., Fitch I.T.,
RA Carbon J., Saunders W.S.;
RT "Slk19p is a centromere protein that functions to stabilize mitotic
RT spindles.";
RL J. Cell Biol. 146:415-425(1999).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11050386; DOI=10.1016/s0960-9822(00)00723-5;
RA Kamieniecki R.J., Shanks R.M.Q., Dawson D.S.;
RT "Slk19p is necessary to prevent separation of sister chromatids in meiosis
RT I.";
RL Curr. Biol. 10:1182-1190(2000).
RN [5]
RP FUNCTION.
RX PubMed=10835382; DOI=10.1093/genetics/155.2.577;
RA Zeng X., Saunders W.S.;
RT "The Saccharomyces cerevisiae centromere protein Slk19p is required for two
RT successive divisions during meiosis.";
RL Genetics 155:577-587(2000).
RN [6]
RP FUNCTION, CLEAVAGE BY ESP1, SUBCELLULAR LOCATION, PHOSPHORYLATION, AND
RP MUTAGENESIS OF ARG-77 AND SER-78.
RX PubMed=11533655; DOI=10.1038/ncb0901-771;
RA Sullivan M., Lehane C., Uhlmann F.;
RT "Orchestrating anaphase and mitotic exit: separase cleavage and
RT localization of Slk19.";
RL Nat. Cell Biol. 3:771-777(2001).
RN [7]
RP FUNCTION AS A COMPONENT OF THE FEAR NETWORK.
RX PubMed=11832211; DOI=10.1016/s0092-8674(02)00618-9;
RA Stegmeier F., Visintin R., Amon A.;
RT "Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function
RT in a network that controls Cdc14 localization during early anaphase.";
RL Cell 108:207-220(2002).
RN [8]
RP FUNCTION.
RX PubMed=12354097; DOI=10.1046/j.1365-2443.2002.00583.x;
RA Pfiz S., Zimmermann J., Hilt W.;
RT "The yeast kinetochore protein Slk19 is required to prevent aberrant
RT chromosome segregation in meiosis and mitosis.";
RL Genes Cells 7:1033-1042(2002).
RN [9]
RP FUNCTION AS A COMPONENT OF THE FEAR NETWORK, AND CLEAVAGE BY ESP1.
RX PubMed=12737807; DOI=10.1016/s1534-5807(03)00129-1;
RA Buonomo S.B., Rabitsch K.P., Fuchs J., Gruber S., Sullivan M., Uhlmann F.,
RA Petronczki M., Toth A., Nasmyth K.;
RT "Division of the nucleolus and its release of CDC14 during anaphase of
RT meiosis I depends on separase, SPO12, and SLK19.";
RL Dev. Cell 4:727-739(2003).
RN [10]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [11]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [12]
RP PHOSPHORYLATION BY CDC28.
RX PubMed=14574415; DOI=10.1038/nature02062;
RA Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K.,
RA Shokat K.M., Morgan D.O.;
RT "Targets of the cyclin-dependent kinase Cdk1.";
RL Nature 425:859-864(2003).
RN [13]
RP SUBCELLULAR LOCATION.
RX PubMed=15282802; DOI=10.1002/yea.1133;
RA Sundin B.A., Chiu C.-H., Riffle M., Davis T.N., Muller E.G.D.;
RT "Localization of proteins that are coordinately expressed with Cln2 during
RT the cell cycle.";
RL Yeast 21:793-800(2004).
RN [14]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT "Analysis of phosphorylation sites on proteins from Saccharomyces
RT cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN [15]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188; SER-189; SER-201;
RP SER-216 AND THR-273, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [16]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188; SER-189; SER-216 AND
RP SER-283, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [17]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Has a role in spindle assembly and stability. Required to
CC ensure a timely exit form mitosis. Essential to maintain pre-anaphase
CC spindle polarity. Associates to the plus ends of the microtubules at
CC the kinetochore and spindle midzone. A component of the FEAR (CDC14
CC Early Anaphase Release) network which promotes CDC14 release from the
CC nucleolus during early anaphase. Required for proper chromosome
CC segregation during meiosis I where it prevents premature sister
CC chromatid separation. {ECO:0000269|PubMed:10427094,
CC ECO:0000269|PubMed:10835382, ECO:0000269|PubMed:11050386,
CC ECO:0000269|PubMed:11533655, ECO:0000269|PubMed:11832211,
CC ECO:0000269|PubMed:12354097, ECO:0000269|PubMed:12737807}.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere. Chromosome, centromere,
CC kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center,
CC spindle pole body. Note=Spindle midzone during anaphase. During meiotic
CC prophase localizes to the centromere and remains there until anaphase
CC I.
CC -!- PTM: Cleaved by ESP1 at the onset of anaphase.
CC -!- PTM: Phosphorylated by CDC5/Polo-like kinase at the onset of anaphase.
CC Phosphorylation takes places at proximity to cleavage sites and is
CC required for an efficient cleavage by ESP1. Phosphorylated also by
CC CDC28. {ECO:0000269|PubMed:11533655, ECO:0000269|PubMed:12737807,
CC ECO:0000269|PubMed:14574415}.
CC -!- MISCELLANEOUS: Present with 314 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z75103; CAA99408.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10970.1; -; Genomic_DNA.
DR PIR; S67087; S67087.
DR RefSeq; NP_014838.3; NM_001183614.3.
DR AlphaFoldDB; Q08581; -.
DR SMR; Q08581; -.
DR BioGRID; 34593; 238.
DR DIP; DIP-2595N; -.
DR IntAct; Q08581; 5.
DR MINT; Q08581; -.
DR STRING; 4932.YOR195W; -.
DR iPTMnet; Q08581; -.
DR MaxQB; Q08581; -.
DR PaxDb; Q08581; -.
DR PRIDE; Q08581; -.
DR EnsemblFungi; YOR195W_mRNA; YOR195W; YOR195W.
DR GeneID; 854370; -.
DR KEGG; sce:YOR195W; -.
DR SGD; S000005721; SLK19.
DR VEuPathDB; FungiDB:YOR195W; -.
DR eggNOG; ENOG502QV33; Eukaryota.
DR HOGENOM; CLU_018393_0_0_1; -.
DR InParanoid; Q08581; -.
DR OMA; SEDMIHF; -.
DR BioCyc; YEAST:G3O-33704-MON; -.
DR PRO; PR:Q08581; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q08581; protein.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000776; C:kinetochore; IDA:SGD.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005819; C:spindle; IDA:SGD.
DR GO; GO:0051233; C:spindle midzone; IDA:SGD.
DR GO; GO:0005816; C:spindle pole body; IEA:UniProtKB-SubCell.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0098653; P:centromere clustering; IMP:SGD.
DR GO; GO:0007059; P:chromosome segregation; IMP:SGD.
DR GO; GO:0051321; P:meiotic cell cycle; IMP:SGD.
DR GO; GO:0007052; P:mitotic spindle organization; IMP:SGD.
DR GO; GO:0007097; P:nuclear migration; IMP:SGD.
DR GO; GO:0031536; P:positive regulation of exit from mitosis; IMP:SGD.
DR InterPro; IPR024312; TACC_fungi.
DR Pfam; PF12709; Fungal_TACC; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore; Meiosis; Microtubule;
KW Mitosis; Phosphoprotein; Reference proteome.
FT CHAIN 1..821
FT /note="Kinetochore protein SLK19"
FT /id="PRO_0000270573"
FT REGION 1..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 99..153
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 274..298
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 699..720
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 310..821
FT /evidence="ECO:0000255"
FT COMPBIAS 11..51
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 99..117
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 135..153
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 283..298
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 77..78
FT /note="Cleavage; by ESP1"
FT MOD_RES 7
FT /note="Phosphothreonine; by CDC28"
FT /evidence="ECO:0000305"
FT MOD_RES 188
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 189
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 201
FT /note="Phosphoserine; by CDC28"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 216
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 273
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 283
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MUTAGEN 77
FT /note="R->E: No cleavage by ESP1."
FT /evidence="ECO:0000269|PubMed:11533655"
FT MUTAGEN 78
FT /note="S->R: Destabilizes; not detectable in anaphase
FT cells; spindle becomes thin and fragile."
FT /evidence="ECO:0000269|PubMed:11533655"
SQ SEQUENCE 821 AA; 95380 MW; 994CCE2EFB7C4B67 CRC64;
MNEVPTTPVR LILGQAQQRE QNSENCSQER NPRTFNSEPD SSFNSPGSSQ FVIHPHEPLE
KEKDEKQDLD RSIDYGRSSA LNNKNNANPL ENIDINKMFD DKKSDSGTND DKGGASTSDK
HVLALNYSPI RVEMNSSEKR SDKNVDVDEN DKEGSHINKK LKLQLESVPD LKQSSTKDII
NDKEEIMSSP MAIDMIETNI SPNKFIINDG VERNDSFNIN TDTLKLENDI NEKQQEEDFI
KSNSNNVVNI DNAYKEKEDE ENDITNSHIN RLTPLYETSA RESNSNEEGR NDYDDDNQLD
IRHDNFQIVA KRNEELTDQI YHLNQMLNSL ISKNESLSFQ YEKLNKNHQL LIDLTNEKLD
KLNTERESDI AKVEKFKKRI KELNTEIKVL NSNQKILQEK FDASITEVNH IKGEHENTVN
TLQQNEKILN DKNVELENMK AELKGNNDKL SEYETTLNDL NSRIVQLNDK IESTDIVLKS
KENELDNLKL SLKETLSISK DFNDSDLIGQ INELISTKNN LQQKMDDLNN LNDDNLKVVQ
DKLIKNEETL KLKEAEIDSL NSEMDELKKQ ITSKDDEFKM WQSKYETVED EAKIRNAEVT
ELNGDIEDLK ESKLHLEETI TELENKVHKL ENECELEKQK FEKTSLELES LQLKNSNIQA
EHIKELENLH ENLISLQNEL KISSDRITTL TKENEVLMEQ NNNNNNSVTL SNDQKDRDDE
KIKSLGKQVQ DWKEKYEAKE KDTNKRLKLL AEDLYIQYSS KHEQKVKLLK KGYENKYQNK
FDQLNLENKT LSEEIEQLNK QLSSEREEKQ ELLKLLENEK K
