SLK1_ARATH
ID SLK1_ARATH Reviewed; 748 AA.
AC Q0WVM7; O65609; Q9M0K8;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Probable transcriptional regulator SLK1;
DE Short=AtSLK1;
DE AltName: Full=Protein SEUSS-like 1;
GN Name=SLK1; OrderedLocusNames=At4g25520; ORFNames=M7J2.110;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=20007451; DOI=10.1104/pp.109.146183;
RA Bao F., Azhakanandam S., Franks R.G.;
RT "SEUSS and SEUSS-LIKE transcriptional adaptors regulate floral and
RT embryonic development in Arabidopsis.";
RL Plant Physiol. 152:821-836(2010).
RN [6]
RP FUNCTION IN ABIOTIC STRESSES, DISRUPTION PHENOTYPE, AND INTERACTION WITH
RP LUH.
RX PubMed=24564815; DOI=10.1186/1471-2229-14-54;
RA Shrestha B., Guragain B., Sridhar V.V.;
RT "Involvement of co-repressor LUH and the adapter proteins SLK1 and SLK2 in
RT the regulation of abiotic stress response genes in Arabidopsis.";
RL BMC Plant Biol. 14:54-54(2014).
CC -!- FUNCTION: Probable transcription regulator that functions in the
CC development of the carpel margin meristem similarly to SEUSS (SEU). In
CC association with SEU, supports organ development from meristematic
CC regions by facilitating auxin response and thus organ initiation, and
CC by sustaining meristematic potential through the maintenance of
CC PHABULOSA expression (PubMed:20007451). DNA-binding adapter subunit of
CC the SEU-SLK1 transcriptional corepressor of abiotic stress (e.g. salt
CC and osmotic stress) response genes (PubMed:24564815).
CC {ECO:0000269|PubMed:20007451, ECO:0000269|PubMed:24564815}.
CC -!- SUBUNIT: Forms corepressor complexes with LUH; LUH is the transcription
CC repressor subunit and SLK1 the specific DNA-binding adapters.
CC {ECO:0000269|PubMed:24564815}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in young flower meristems, ovules and the
CC carpel margin meristem. {ECO:0000269|PubMed:20007451}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but the double mutants seu and slk1 are short in stature,
CC sterile and display strong disruptions of floral development and high
CC frequency of female gametophyte disruption (PubMed:20007451). Enhanced
CC tolerance to salt and osmotic stress conditions (PubMed:24564815).
CC {ECO:0000269|PubMed:20007451, ECO:0000269|PubMed:24564815}.
CC -!- SIMILARITY: Belongs to the adn1/SEU family. {ECO:0000305}.
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DR EMBL; AL022197; CAA18174.1; -; Genomic_DNA.
DR EMBL; AL161563; CAB81362.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85073.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM67173.1; -; Genomic_DNA.
DR EMBL; BT012580; AAS99724.1; -; mRNA.
DR EMBL; AK226715; BAE98821.1; -; mRNA.
DR PIR; H85294; H85294.
DR PIR; T05795; T05795.
DR RefSeq; NP_001320064.1; NM_001341749.1.
DR RefSeq; NP_194282.2; NM_118684.9.
DR AlphaFoldDB; Q0WVM7; -.
DR BioGRID; 13944; 1.
DR STRING; 3702.AT4G25520.1; -.
DR iPTMnet; Q0WVM7; -.
DR PaxDb; Q0WVM7; -.
DR PRIDE; Q0WVM7; -.
DR ProteomicsDB; 228440; -.
DR EnsemblPlants; AT4G25520.1; AT4G25520.1; AT4G25520.
DR EnsemblPlants; AT4G25520.2; AT4G25520.2; AT4G25520.
DR GeneID; 828657; -.
DR Gramene; AT4G25520.1; AT4G25520.1; AT4G25520.
DR Gramene; AT4G25520.2; AT4G25520.2; AT4G25520.
DR KEGG; ath:AT4G25520; -.
DR Araport; AT4G25520; -.
DR TAIR; locus:2131804; AT4G25520.
DR eggNOG; ENOG502SZ3H; Eukaryota.
DR HOGENOM; CLU_007007_1_0_1; -.
DR InParanoid; Q0WVM7; -.
DR OMA; GINNNMR; -.
DR OrthoDB; 933055at2759; -.
DR PhylomeDB; Q0WVM7; -.
DR PRO; PR:Q0WVM7; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q0WVM7; baseline and differential.
DR Genevisible; Q0WVM7; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0048467; P:gynoecium development; IMP:TAIR.
DR GO; GO:1901001; P:negative regulation of response to salt stress; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048481; P:plant ovule development; IMP:TAIR.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0047484; P:regulation of response to osmotic stress; IMP:UniProtKB.
DR InterPro; IPR029005; LIM-bd/SEUSS.
DR PANTHER; PTHR10378; PTHR10378; 2.
DR Pfam; PF01803; LIM_bind; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Flowering; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..748
FT /note="Probable transcriptional regulator SLK1"
FT /id="PRO_0000430163"
FT REGION 67..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 138..163
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 204..451
FT /note="Dimerization"
FT /evidence="ECO:0000250"
FT REGION 572..653
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 667..712
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 213..227
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 67..91
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 93
FT /note="N -> S (in Ref. 1; CAA18174/CAB81362 and 3;
FT AAS99724)"
FT /evidence="ECO:0000305"
FT CONFLICT 394
FT /note="M -> R (in Ref. 1; CAB81362)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 748 AA; 83314 MW; 7518457A94086017 CRC64;
MNRTVVSGAV ESSFSLTDAV GTEALNMQRS SGINNNMRIP TSPMSFSSNS VNIPGSLVLD
GSAASMQHLP QQQQQQLLQQ QTGQGSVPMR ENNYSHVDKK PRLEVKQEDM LQQQILQQLI
QRQDPTGRNP QMQALLQQQR LRQHQQMLQS MSPSQRLQLQ QQQQLRQQLQ QQGTQQIPPN
VRPYEVGVCA RKLMMYLYHL QQRPAENCIT YWRKFVAEYF SPRAKQRLCL SQYESAGHHA
LGMFPQAAPD MWQCDLCGTK SGKGFEATFD VLARLIEIKF ASGIIDELLY LDHPRENRFP
NGLMMLEYRK AVQETVHEQF RVVREGHLRI IFSQDLKILS WEFCARRHEE LLLRRLIAPQ
VNQLLQVAQK CQSTISESGS EGVSQQDLQS NSNMVLGAGR QLAKFMELQS LNDLGYPKRY
IRTLQISEVV KSMKDLMNFT GEQKIGPIEG LKRLLEQTVT VKLQKQKMQE MEQFGNNGAI
NGPVQAQMVL TSGTMNGSTG NNTNNHHQIV GRGAMSGPAE GQMVISSGTV SGATANNNSN
NHNQIVGRGA MNGSAQAAAA LTNYQSMLMR QNAMNNPNSN TGKQEGFSSQ NPTPNSNQSP
SSSSQQRHNL VTGGFPNSPQ MQQQQRTMNG PTNILPQNHP HQLQSPHSHG NTPEQQMLHQ
LLQEMSENGG SVQQQQAFSG QSGSNSNAER NTTASTSNIS GGGRAPSRNN SFKAASNNNL
HFSEDISITD HDFSEDGFFN NNDIYGGL
