SLK2_ARATH
ID SLK2_ARATH Reviewed; 816 AA.
AC Q94BP0; Q9FIS9;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Probable transcriptional regulator SLK2;
DE Short=AtSLK2;
DE AltName: Full=Protein SEUSS-like 2;
GN Name=SLK2; OrderedLocusNames=At5g62090; ORFNames=MTG10.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=20007451; DOI=10.1104/pp.109.146183;
RA Bao F., Azhakanandam S., Franks R.G.;
RT "SEUSS and SEUSS-LIKE transcriptional adaptors regulate floral and
RT embryonic development in Arabidopsis.";
RL Plant Physiol. 152:821-836(2010).
RN [5]
RP FUNCTION IN ABIOTIC STRESSES, DISRUPTION PHENOTYPE, AND INTERACTION WITH
RP LUH.
RX PubMed=24564815; DOI=10.1186/1471-2229-14-54;
RA Shrestha B., Guragain B., Sridhar V.V.;
RT "Involvement of co-repressor LUH and the adapter proteins SLK1 and SLK2 in
RT the regulation of abiotic stress response genes in Arabidopsis.";
RL BMC Plant Biol. 14:54-54(2014).
CC -!- FUNCTION: Probable transcription regulator that functions in the
CC development of the carpel margin meristem similarly to SEUSS (SEU). In
CC association with SEU, supports organ development from meristematic
CC regions by facilitating auxin response and thus organ initiation, and
CC by sustaining meristematic potential through the maintenance of
CC PHABULOSA expression (PubMed:20007451). DNA-binding adapter subunit of
CC the SEU-SLK2 transcriptional corepressor of abiotic stress (e.g. salt
CC and osmotic stress) response genes (PubMed:24564815).
CC {ECO:0000269|PubMed:20007451, ECO:0000269|PubMed:24564815}.
CC -!- SUBUNIT: Forms corepressor complexes with LUH; LUH is the transcription
CC repressor subunit and SLK2 the specific DNA-binding adapters.
CC {ECO:0000269|PubMed:24564815}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in young flower meristems, ovules and the
CC carpel margin meristem. {ECO:0000269|PubMed:20007451}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but the double mutants seu and slk2 show severe embryonic
CC and seedling defects characterized by a loss of all structures derived
CC from the shoot apical meristem (SAM) (PubMed:20007451). Enhanced
CC tolerance to salt and osmotic stress conditions (PubMed:24564815).
CC {ECO:0000269|PubMed:20007451, ECO:0000269|PubMed:24564815}.
CC -!- SIMILARITY: Belongs to the adn1/SEU family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10171.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB016880; BAB10171.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002688; AED97560.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97561.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70821.1; -; Genomic_DNA.
DR EMBL; AY039981; AAK64158.1; -; mRNA.
DR EMBL; AY150458; AAN12899.1; -; mRNA.
DR RefSeq; NP_001332402.1; NM_001345520.1.
DR RefSeq; NP_201015.1; NM_125602.3.
DR RefSeq; NP_851245.1; NM_180914.2.
DR AlphaFoldDB; Q94BP0; -.
DR BioGRID; 21573; 10.
DR IntAct; Q94BP0; 7.
DR STRING; 3702.AT5G62090.1; -.
DR iPTMnet; Q94BP0; -.
DR PaxDb; Q94BP0; -.
DR PRIDE; Q94BP0; -.
DR ProteomicsDB; 232560; -.
DR EnsemblPlants; AT5G62090.1; AT5G62090.1; AT5G62090.
DR EnsemblPlants; AT5G62090.2; AT5G62090.2; AT5G62090.
DR EnsemblPlants; AT5G62090.3; AT5G62090.3; AT5G62090.
DR GeneID; 836329; -.
DR Gramene; AT5G62090.1; AT5G62090.1; AT5G62090.
DR Gramene; AT5G62090.2; AT5G62090.2; AT5G62090.
DR Gramene; AT5G62090.3; AT5G62090.3; AT5G62090.
DR KEGG; ath:AT5G62090; -.
DR Araport; AT5G62090; -.
DR TAIR; locus:2174028; AT5G62090.
DR eggNOG; ENOG502QURB; Eukaryota.
DR HOGENOM; CLU_007007_1_0_1; -.
DR InParanoid; Q94BP0; -.
DR OMA; SGVQQQC; -.
DR OrthoDB; 933055at2759; -.
DR PhylomeDB; Q94BP0; -.
DR PRO; PR:Q94BP0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q94BP0; baseline and differential.
DR Genevisible; Q94BP0; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0048467; P:gynoecium development; IMP:TAIR.
DR GO; GO:1901001; P:negative regulation of response to salt stress; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048481; P:plant ovule development; IMP:TAIR.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0047484; P:regulation of response to osmotic stress; IMP:UniProtKB.
DR GO; GO:0010044; P:response to aluminum ion; IMP:TAIR.
DR InterPro; IPR029005; LIM-bd/SEUSS.
DR PANTHER; PTHR10378; PTHR10378; 2.
DR Pfam; PF01803; LIM_bind; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Flowering; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..816
FT /note="Probable transcriptional regulator SLK2"
FT /id="PRO_0000430164"
FT REGION 133..153
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 166..189
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 307..554
FT /note="Dimerization"
FT /evidence="ECO:0000250"
FT REGION 644..711
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 316..330
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
SQ SEQUENCE 816 AA; 90548 MW; 4D074BA67F097782 CRC64;
MASSTSGIFF QGDDESQSFI NSHLTSSYGN SSNSAPGCGG PTGGYHNLSM VSGDMHNPVM
MSVSTPGPSA GASSLVTDAN SGLSGGGPHL QRSASINNES YMRLPASPMS FSSNNISISG
SSVVDGSTVV QRHDPSVQLG GSSATSLPTS QTNQIPLSMA RRASESFFQD PNNLTQARKK
PRLDSKQDDA LQQQILRQWL QRQDILQQQQ QQQQQGQNPQ FQILLQQQKL RQQQQYLQSL
PPLQRVQLQQ QQQVQQQQQL QQQHQQQQQQ LQQQGMQMQL TGGPRPYENS VCARRLMQYL
YHQRQRPSES SIVYWRKFVT EYFSPRAKKR WCLSHYDNVG HSALGVSPQA ATDEWQCDLC
GSKSGRGFEA TFDVLPRLNE IKFASGVLDE LLYLGVPSER RYGSGIMVLE YGKAVQESVY
EHIRVVREGH LRIIFSQELK ILSWEFCTRR HEELLPRRLV APQVNQLLQV AEKCQSTIDQ
SGSDGIHQQD LQANSNMVMA AGRQLAKSLE SHSLNDLGFS KRYVRCLQIS EVVSSMKDMI
DFCRDQKVGP IEALKSYPYR MKAGKPQMQE MEQLAAARGL PPDRNSLNKL MALRNSGINI
PMNNMSGQGS LPGSAQAAAF ALTNYQSMLM KQNHLNSDLN NTTIQQEPSR NRSASPSYQG
TSPLLPGFVH SPSISGVSSH LSPQRQMPSS SYNGSTQQYH QQPPSCSSGN QTLEQQMIHQ
IWQQMANSNG GSGQQQQSLS GQNMMNCNTN MGRNRTDYVP AAAETPSTSN RFRGIKGLDQ
SQNLEGIISN TSLNFGNNGV FSNEVDESMG GYSWKS
