SLM2_NEIMB
ID SLM2_NEIMB Reviewed; 505 AA.
AC Q9JXM5;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Surface lipoprotein assembly modifier 2 {ECO:0000303|PubMed:27572441};
DE Short=Slam2 {ECO:0000303|PubMed:27572441};
DE Flags: Precursor;
GN OrderedLocusNames=NMB1971;
OS Neisseria meningitidis serogroup B (strain MC58).
OC Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC Neisseria.
OX NCBI_TaxID=122586;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MC58;
RX PubMed=10710307; DOI=10.1126/science.287.5459.1809;
RA Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E.,
RA Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C.,
RA Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H.,
RA Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T.M.,
RA Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D.,
RA Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V.,
RA Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M.,
RA Moxon E.R., Rappuoli R., Venter J.C.;
RT "Complete genome sequence of Neisseria meningitidis serogroup B strain
RT MC58.";
RL Science 287:1809-1815(2000).
RN [2]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=MC58;
RX PubMed=27572441; DOI=10.1038/nmicrobiol.2016.9;
RA Hooda Y., Lai C.C., Judd A., Buckwalter C.M., Shin H.E., Gray-Owen S.D.,
RA Moraes T.F.;
RT "Slam is an outer membrane protein that is required for the surface display
RT of lipidated virulence factors in Neisseria.";
RL Nat. Microbiol. 1:16009-16009(2016).
CC -!- FUNCTION: Required for correct export to the cell surface of cell outer
CC membrane lipoprotein HpuA heterologously in E.coli (hpuA does not exist
CC in N.meningitidis strain MC58). {ECO:0000269|PubMed:27572441}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000305|PubMed:27572441}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DOMAIN: Consists of a soluble N-terminal domain and C-terminal probable
CC beta-barrel in the outer membrane with 14 predicted beta-strands.
CC {ECO:0000250|UniProtKB:Q9K165}.
CC -!- SIMILARITY: Belongs to the Slam family. {ECO:0000305}.
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DR EMBL; AE002098; AAF42300.1; -; Genomic_DNA.
DR PIR; B81021; B81021.
DR RefSeq; NP_274965.1; NC_003112.2.
DR RefSeq; WP_002244327.1; NC_003112.2.
DR AlphaFoldDB; Q9JXM5; -.
DR STRING; 122586.NMB1971; -.
DR PaxDb; Q9JXM5; -.
DR EnsemblBacteria; AAF42300; AAF42300; NMB1971.
DR KEGG; nme:NMB1971; -.
DR PATRIC; fig|122586.8.peg.2510; -.
DR HOGENOM; CLU_034927_1_0_4; -.
DR OMA; YAIEGHH; -.
DR Proteomes; UP000000425; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR007655; Slam.
DR Pfam; PF04575; SlipAM; 1.
PE 3: Inferred from homology;
KW Cell outer membrane; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane beta strand.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..505
FT /note="Surface lipoprotein assembly modifier 2"
FT /evidence="ECO:0000255"
FT /id="PRO_5004327679"
FT TRANSMEM 190..200
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..243
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..258
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..283
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..297
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..335
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..350
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..377
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..391
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..420
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 427..437
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 456..465
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 472..482
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 495..505
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT REGION 23..188
FT /note="N-terminal domain"
FT /evidence="ECO:0000250|UniProtKB:Q9K165"
FT REGION 189..505
FT /note="C-terminal probable beta barrel"
FT /evidence="ECO:0000250|UniProtKB:Q9K165"
SQ SEQUENCE 505 AA; 56756 MW; A631E22A120B52B8 CRC64;
MLYFRYGFLV VWCAAGVSAA YGADAPAILD DKALLQVQRS VSDKWAESDW KVENDAPRVV
DGDFLLAHPK MLEHSLRDAL NGNQADLIAS LADLYAKLPD YDAVLYGRAR ALLAKLAGRP
AEAVARYREL HGENAADERI LLDLAAAEFD DFRLKSAERH FAEAAKLDLP APVLENVGRF
RKKTEGLTGW RFSGGISPAV NRNANNAAPQ YCRQNGGRQI CSVSRAERAA GLNYEIEAEK
LTPLADNHYL LFRSNIGGTS YYFSKKSAYD DGFGRAYLGW QYKNARQTAG ILPFYQVQLS
GSDGFDAKTK RVNNRRLPPY MLAHGVGVQL SHTYRPNPGW QFSVALEHYR QRYREQDRAE
YNNGRQDGFY VSSAKRLGES ATVFGGWQFV RFVPKRETVG GAVNNAAYRR NGVYAGWAQE
WRQLGGLNSR VSASYARRNY KGIAAFSTEA QRNREWNVSL ALSHDKLSYK GIVPALNYRF
GRTESNVPYA KRRNSEVFVS ADWRF
