SLM4_YEAST
ID SLM4_YEAST Reviewed; 162 AA.
AC P38247; D6VQ76;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Protein SLM4;
DE AltName: Full=EGO complex subunit 3;
DE AltName: Full=GSE complex subunit 1;
GN Name=SLM4; Synonyms=EGO3, GSE1; OrderedLocusNames=YBR077C;
GN ORFNames=YBR0723;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7985423; DOI=10.1002/yea.320100711;
RA van der Aart Q.J.M., Barthe C., Doignon F., Aigle M., Crouzet M.,
RA Steensma H.Y.;
RT "Sequence analysis of a 31 kb DNA fragment from the right arm of
RT Saccharomyces cerevisiae chromosome II.";
RL Yeast 10:959-964(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP FUNCTION, IDENTIFICATION IN THE EGO COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=15989961; DOI=10.1016/j.molcel.2005.05.020;
RA Dubouloz F., Deloche O., Wanke V., Cameroni E., De Virgilio C.;
RT "The TOR and EGO protein complexes orchestrate microautophagy in yeast.";
RL Mol. Cell 19:15-26(2005).
RN [7]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE
RP GSE COMPLEX.
RX PubMed=16732272; DOI=10.1038/ncb1419;
RA Gao M., Kaiser C.A.;
RT "A conserved GTPase-containing complex is required for intracellular
RT sorting of the general amino-acid permease in yeast.";
RL Nat. Cell Biol. 8:657-667(2006).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=29698392; DOI=10.1371/journal.pgen.1007334;
RA Ukai H., Araki Y., Kira S., Oikawa Y., May A.I., Noda T.;
RT "Gtr/Ego-independent TORC1 activation is achieved through a glutamine-
RT sensitive interaction with Pib2 on the vacuolar membrane.";
RL PLoS Genet. 14:e1007334-e1007334(2018).
RN [9]
RP DISRUPTION PHENOTYPE.
RX PubMed=32801125; DOI=10.1242/jcs.245555;
RA Uemura S., Mochizuki T., Amemiya K., Kurosaka G., Yazawa M., Nakamoto K.,
RA Ishikawa Y., Izawa S., Abe F.;
RT "Amino acid homeostatic control by TORC1 in Saccharomyces cerevisiae under
RT high hydrostatic pressure.";
RL J. Cell Sci. 133:jcs245555-jcs245555(2020).
RN [10]
RP X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS), AND INTERACTION WITH MEH1.
RX PubMed=20655927; DOI=10.1016/j.jmb.2010.07.034;
RA Kogan K., Spear E.D., Kaiser C.A., Fass D.;
RT "Structural conservation of components in the amino acid sensing branch of
RT the TOR pathway in yeast and mammals.";
RL J. Mol. Biol. 402:388-398(2010).
CC -!- FUNCTION: Component of the GSE complex, a GTPase complex required for
CC intracellular sorting of GAP1 out of the endosome (PubMed:16732272).
CC Component of the EGO complex, a complex involved in the regulation of
CC microautophagy (PubMed:15989961). {ECO:0000269|PubMed:15989961,
CC ECO:0000269|PubMed:16732272}.
CC -!- SUBUNIT: Component of the GSE complex composed of GTR1, GTR2, SLM4,
CC MEH1 and LTV1 (PubMed:16732272). Component of the EGO complex, at least
CC composed of GTR2, SLM4 and MEH1 (PubMed:15989961).
CC {ECO:0000269|PubMed:15989961, ECO:0000269|PubMed:16732272}.
CC -!- INTERACTION:
CC P38247; Q00582: GTR1; NbExp=5; IntAct=EBI-21507, EBI-7954;
CC P38247; Q02205: MEH1; NbExp=4; IntAct=EBI-21507, EBI-27062;
CC P38247; P38247: SLM4; NbExp=3; IntAct=EBI-21507, EBI-21507;
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:15989961, ECO:0000269|PubMed:29698392}; Single-pass
CC membrane protein {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:15989961}.
CC -!- DISRUPTION PHENOTYPE: Abnormal activation of TORC1 signaling
CC (PubMed:32801125). Abnormal punctate localization of TOR1
CC (PubMed:32801125). Sensitive to high hydrostatic pressure (mechanical
CC stress) (PubMed:32801125). {ECO:0000269|PubMed:32801125}.
CC -!- MISCELLANEOUS: Present with 3490 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X76294; CAA53934.1; -; Genomic_DNA.
DR EMBL; Z35946; CAA85021.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07196.1; -; Genomic_DNA.
DR PIR; S45472; S45472.
DR RefSeq; NP_009633.3; NM_001178425.3.
DR PDB; 3LGO; X-ray; 2.85 A; A=1-162.
DR PDB; 4FTX; X-ray; 2.10 A; A/B=1-162.
DR PDB; 4FUW; X-ray; 2.60 A; A/B=1-162.
DR PDB; 4XPM; X-ray; 2.40 A; C=1-162.
DR PDB; 6JWP; X-ray; 3.20 A; E/J=1-162.
DR PDBsum; 3LGO; -.
DR PDBsum; 4FTX; -.
DR PDBsum; 4FUW; -.
DR PDBsum; 4XPM; -.
DR PDBsum; 6JWP; -.
DR AlphaFoldDB; P38247; -.
DR SMR; P38247; -.
DR BioGRID; 32779; 111.
DR ComplexPortal; CPX-3172; EGO complex.
DR ComplexPortal; CPX-3233; GSE complex.
DR DIP; DIP-1763N; -.
DR IntAct; P38247; 8.
DR MINT; P38247; -.
DR STRING; 4932.YBR077C; -.
DR MaxQB; P38247; -.
DR PaxDb; P38247; -.
DR PRIDE; P38247; -.
DR EnsemblFungi; YBR077C_mRNA; YBR077C; YBR077C.
DR GeneID; 852369; -.
DR KEGG; sce:YBR077C; -.
DR SGD; S000000281; SLM4.
DR VEuPathDB; FungiDB:YBR077C; -.
DR eggNOG; ENOG502S129; Eukaryota.
DR HOGENOM; CLU_104687_0_0_1; -.
DR InParanoid; P38247; -.
DR OMA; HTCVAQI; -.
DR BioCyc; YEAST:G3O-29045-MON; -.
DR EvolutionaryTrace; P38247; -.
DR PRO; PR:P38247; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38247; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005770; C:late endosome; IDA:ComplexPortal.
DR GO; GO:0031902; C:late endosome membrane; IDA:SGD.
DR GO; GO:0071986; C:Ragulator complex; IDA:SGD.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0032456; P:endocytic recycling; IDA:ComplexPortal.
DR GO; GO:0016237; P:lysosomal microautophagy; IMP:SGD.
DR GO; GO:0032008; P:positive regulation of TOR signaling; IC:ComplexPortal.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0007165; P:signal transduction; IMP:SGD.
DR InterPro; IPR020233; Slm4.
DR Pfam; PF16818; SLM4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Autophagy; Membrane; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..162
FT /note="Protein SLM4"
FT /id="PRO_0000202479"
FT TRANSMEM 127..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 9..17
FT /evidence="ECO:0007829|PDB:4FTX"
FT HELIX 22..24
FT /evidence="ECO:0007829|PDB:4FTX"
FT STRAND 33..39
FT /evidence="ECO:0007829|PDB:4FTX"
FT TURN 40..42
FT /evidence="ECO:0007829|PDB:4FTX"
FT STRAND 45..50
FT /evidence="ECO:0007829|PDB:4FTX"
FT STRAND 53..55
FT /evidence="ECO:0007829|PDB:4FTX"
FT HELIX 59..84
FT /evidence="ECO:0007829|PDB:4FTX"
FT STRAND 92..100
FT /evidence="ECO:0007829|PDB:4FTX"
FT STRAND 103..112
FT /evidence="ECO:0007829|PDB:4FTX"
FT STRAND 115..121
FT /evidence="ECO:0007829|PDB:4FTX"
FT STRAND 125..134
FT /evidence="ECO:0007829|PDB:4FTX"
FT HELIX 139..150
FT /evidence="ECO:0007829|PDB:4FTX"
FT HELIX 151..156
FT /evidence="ECO:0007829|PDB:4FTX"
SQ SEQUENCE 162 AA; 18354 MW; D64ABDE1CDF265FD CRC64;
MVMLHSKNVK GFLENTLKPY DLHSVDFKTS SLQSSMIITA TNGGILSYAT SNNDVPKNSI
NEINSVNNLK MMSLLIKDKW SEDENDTEEQ HSNSCYPVEI DSFKTKIYTY EMEDLHTCVA
QIPNSDLLLL FIAEGSFPYG LLVIKIERAM RELTDLFGYK LG
