SLMA_VIBCH
ID SLMA_VIBCH Reviewed; 196 AA.
AC Q9KVD2;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2005, sequence version 2.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Nucleoid occlusion factor SlmA {ECO:0000255|HAMAP-Rule:MF_01839};
GN Name=slmA {ECO:0000255|HAMAP-Rule:MF_01839}; OrderedLocusNames=VC_0214;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
CC -!- FUNCTION: Required for nucleoid occlusion (NO) phenomenon, which
CC prevents Z-ring formation and cell division over the nucleoid. Acts as
CC a DNA-associated cell division inhibitor that binds simultaneously
CC chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers.
CC SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of
CC the chromosome, preventing FtsZ polymerization at these regions.
CC {ECO:0000255|HAMAP-Rule:MF_01839}.
CC -!- SUBUNIT: Homodimer. Interacts with FtsZ. {ECO:0000255|HAMAP-
CC Rule:MF_01839}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid {ECO:0000255|HAMAP-
CC Rule:MF_01839}.
CC -!- SIMILARITY: Belongs to the nucleoid occlusion factor SlmA family.
CC {ECO:0000255|HAMAP-Rule:MF_01839}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF93390.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE003852; AAF93390.1; ALT_INIT; Genomic_DNA.
DR PIR; H82350; H82350.
DR RefSeq; NP_229871.2; NC_002505.1.
DR RefSeq; WP_000918350.1; NZ_LT906614.1.
DR PDB; 4GCT; X-ray; 2.45 A; A/B/C/D=1-196.
DR PDB; 4GFK; X-ray; 1.95 A; A/B=1-196.
DR PDB; 5HAW; X-ray; 1.89 A; A/B=6-196.
DR PDBsum; 4GCT; -.
DR PDBsum; 4GFK; -.
DR PDBsum; 5HAW; -.
DR AlphaFoldDB; Q9KVD2; -.
DR SMR; Q9KVD2; -.
DR STRING; 243277.VC_0214; -.
DR DNASU; 2614568; -.
DR EnsemblBacteria; AAF93390; AAF93390; VC_0214.
DR GeneID; 57738951; -.
DR KEGG; vch:VC_0214; -.
DR PATRIC; fig|243277.26.peg.195; -.
DR eggNOG; COG1309; Bacteria.
DR HOGENOM; CLU_069356_5_0_6; -.
DR OMA; IEQTVFG; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0043590; C:bacterial nucleoid; IEA:UniProtKB-UniRule.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0010974; P:negative regulation of division septum assembly; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR HAMAP; MF_01839; NO_factor_SlmA; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001647; HTH_TetR.
DR InterPro; IPR023769; NO_SlmA.
DR InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR Pfam; PF00440; TetR_N; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF48498; SSF48498; 1.
DR PROSITE; PS50977; HTH_TETR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Coiled coil; Cytoplasm;
KW DNA-binding; Reference proteome.
FT CHAIN 1..196
FT /note="Nucleoid occlusion factor SlmA"
FT /id="PRO_0000198983"
FT DOMAIN 7..68
FT /note="HTH tetR-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01839"
FT DNA_BIND 31..50
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01839"
FT COILED 110..139
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01839"
FT HELIX 9..22
FT /evidence="ECO:0007829|PDB:5HAW"
FT HELIX 24..27
FT /evidence="ECO:0007829|PDB:5HAW"
FT HELIX 32..39
FT /evidence="ECO:0007829|PDB:5HAW"
FT HELIX 43..46
FT /evidence="ECO:0007829|PDB:5HAW"
FT TURN 47..49
FT /evidence="ECO:0007829|PDB:5HAW"
FT HELIX 53..78
FT /evidence="ECO:0007829|PDB:5HAW"
FT HELIX 82..99
FT /evidence="ECO:0007829|PDB:5HAW"
FT HELIX 101..107
FT /evidence="ECO:0007829|PDB:5HAW"
FT HELIX 111..114
FT /evidence="ECO:0007829|PDB:5HAW"
FT HELIX 118..139
FT /evidence="ECO:0007829|PDB:5HAW"
FT HELIX 142..145
FT /evidence="ECO:0007829|PDB:4GCT"
FT HELIX 153..173
FT /evidence="ECO:0007829|PDB:5HAW"
FT TURN 174..176
FT /evidence="ECO:0007829|PDB:5HAW"
FT TURN 180..183
FT /evidence="ECO:0007829|PDB:5HAW"
FT HELIX 184..192
FT /evidence="ECO:0007829|PDB:5HAW"
FT TURN 193..195
FT /evidence="ECO:0007829|PDB:4GFK"
SQ SEQUENCE 196 AA; 22743 MW; D3546115CEC238BD CRC64;
MAGNKKINRR EEILQALAEM LESNEGASRI TTAKLAKQVG VSEAALYRHF PSKARMFEGL
IEFIEESLMS RINRIFDEEK DTLNRIRLVM QLLLAFAERN PGLTRILSGH ALMFENERLR
DRINQLFERI ETSLRQILRE RKLREGKSFP VDENILAAQL LGQVEGSLNR FVRSDFKYLP
TANFDEYWAL LSAQIK
