ID   SLMH_RHIRD              Reviewed;         299 AA.
AC   A6XIG7;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=4-sulfomuconolactone hydrolase;
DE            EC=3.1.1.92;
DE   AltName: Full=4-carboxymethylen-4-sulfo-but-2-en-olide hydrolases;
OS   Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS   radiobacter).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY,
RP   BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RC   STRAIN=S2;
RX   PubMed=17660282; DOI=10.1128/jb.00611-07;
RA   Halak S., Basta T., Burger S., Contzen M., Wray V., Pieper D.H., Stolz A.;
RT   "4-sulfomuconolactone hydrolases from Hydrogenophaga intermedia S1 and
RT   Agrobacterium radiobacter S2.";
RL   J. Bacteriol. 189:6998-7006(2007).
CC   -!- FUNCTION: Involved in the degradation of 4-sulfocatechol which is a
CC       central intermediate in the degradation of substituted sulfonated
CC       benzenes. Catalyzes the hydrolytical desulfonation of 4-
CC       sulfomuconolactone to yield maleylacetate.
CC       {ECO:0000269|PubMed:17660282}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-sulfomuconolactone + H2O = 2 H(+) + maleylacetate + sulfite;
CC         Xref=Rhea:RHEA:33711, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16468, ChEBI:CHEBI:17359, ChEBI:CHEBI:20479; EC=3.1.1.92;
CC         Evidence={ECO:0000269|PubMed:17660282};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.34 mM for 4-sulfomuconolactone (at pH 8)
CC         {ECO:0000269|PubMed:17660282};
CC         Vmax=12.6 umol/min/mg enzyme (at pH 8) {ECO:0000269|PubMed:17660282};
CC         Note=kcat is 400 min(-1) with 4-sulfomuconolactone (at pH 8).;
CC       pH dependence:
CC         Optimum pH is between 7 and 7.5. Approximately 30% of the maximal
CC         activities are still detected at pH 5 and pH 9.
CC         {ECO:0000269|PubMed:17660282};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:17660282}.
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC       Sulfomuconolactone hydrolase family. {ECO:0000305}.
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DR   EMBL; DQ813262; ABH09755.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6XIG7; -.
DR   SMR; A6XIG7; -.
DR   KEGG; ag:ABH09755; -.
DR   BRENDA; 3.1.1.92; 200.
DR   GO; GO:0102998; F:4-sulfomuconolactone hydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IDA:UniProtKB.
DR   GO; GO:0018732; F:sulfolactone hydrolase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   Pfam; PF04909; Amidohydro_2; 1.
DR   SUPFAM; SSF51556; SSF51556; 1.
PE   1: Evidence at protein level;
KW   Hydrolase.
FT   CHAIN           1..299
FT                   /note="4-sulfomuconolactone hydrolase"
FT                   /id="PRO_0000422785"
SQ   SEQUENCE   299 AA;  33289 MW;  A7A176833274ADE4 CRC64;
     MLPADQAGIP PCQGPRARSA PISFAIPKGA WDTHLHVFGP TAVFPYAEKR PYTPPDSPLE
     DYLALMERLG IERGVCVHPN VHGIDNSVTI DAVERSDRRL LGIIKPHRVM TFTELRDLKT
     RGVRGVRFAF NPQHGSGALD TELFERMHGW CRELDWCINM HFAPDALEGL CDLIAGAETP
     IIIDHFGRVE TAAGVNQLPF KILRDLATLD HVWIKLTGAD RISHSGVPYD DVVPFAHALS
     EIAPDRLLWG SDWPHSGYFD PKRMPDDGDL LNLVARFAPD VALRHKILVD NPARLFGVI