SLN13_HUMAN
ID SLN13_HUMAN Reviewed; 897 AA.
AC Q68D06; E1P645; Q658M1; Q6ZS51; Q96A81;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Schlafen family member 13;
DE EC=3.1.-.- {ECO:0000269|PubMed:29563550};
DE AltName: Full=Schlafen-13 {ECO:0000305};
DE Short=hSLFN13 {ECO:0000303|PubMed:29563550};
GN Name=SLFN13 {ECO:0000312|HGNC:HGNC:26481};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Cervix, and Stomach;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, ACTIVE SITE, AND MUTAGENESIS OF GLU-208;
RP GLU-213 AND ASP-251.
RX PubMed=29563550; DOI=10.1038/s41467-018-03544-x;
RA Yang J.Y., Deng X.Y., Li Y.S., Ma X.C., Feng J.X., Yu B., Chen Y.,
RA Luo Y.L., Wang X., Chen M.L., Fang Z.X., Zheng F.X., Li Y.P., Zhong Q.,
RA Kang T.B., Song L.B., Xu R.H., Zeng M.S., Chen W., Zhang H., Xie W.,
RA Gao S.;
RT "Structure of Schlafen13 reveals a new class of tRNA/rRNA- targeting RNase
RT engaged in translational control.";
RL Nat. Commun. 9:1165-1165(2018).
CC -!- FUNCTION: Endoribonuclease that cleaves tRNAs and rRNAs
CC (PubMed:29563550). Cleaves tRNAs 11 nucleotides from the 3'-terminus at
CC the acceptor stem (PubMed:29563550). Does not act on tRNA(Sec)
CC (PubMed:29563550). Able to restrict HIV-1 virus replication; ability to
CC inhibit HIV-1 replication is dependent on endoribonuclease activity
CC (PubMed:29563550). {ECO:0000269|PubMed:29563550}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:Q5U311};
CC Note=Can also use Mn(2+). {ECO:0000250|UniProtKB:Q5U311};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:29563550}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q68D06-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q68D06-2; Sequence=VSP_024273;
CC -!- DOMAIN: Shows a pseudo-dimeric U-pillow-shaped architecture of the
CC SLFN13 N'-domain that may clamp base-paired RNAs.
CC {ECO:0000250|UniProtKB:Q5U311}.
CC -!- SIMILARITY: Belongs to the Schlafen family. Subgroup III subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AK127728; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK056514; BAB71201.1; -; mRNA.
DR EMBL; AK127728; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; CR749630; CAH18424.1; -; mRNA.
DR EMBL; AL833747; CAH56227.1; -; mRNA.
DR EMBL; CH471147; EAW80150.1; -; Genomic_DNA.
DR EMBL; CH471147; EAW80152.1; -; Genomic_DNA.
DR CCDS; CCDS32620.1; -. [Q68D06-1]
DR RefSeq; NP_653283.3; NM_144682.5. [Q68D06-1]
DR RefSeq; XP_005257979.1; XM_005257922.2. [Q68D06-1]
DR RefSeq; XP_016879721.1; XM_017024232.1. [Q68D06-1]
DR RefSeq; XP_016879722.1; XM_017024233.1. [Q68D06-1]
DR AlphaFoldDB; Q68D06; -.
DR SMR; Q68D06; -.
DR BioGRID; 127019; 2.
DR STRING; 9606.ENSP00000285013; -.
DR iPTMnet; Q68D06; -.
DR PhosphoSitePlus; Q68D06; -.
DR BioMuta; SLFN13; -.
DR DMDM; 74708791; -.
DR EPD; Q68D06; -.
DR jPOST; Q68D06; -.
DR MassIVE; Q68D06; -.
DR MaxQB; Q68D06; -.
DR PaxDb; Q68D06; -.
DR PeptideAtlas; Q68D06; -.
DR PRIDE; Q68D06; -.
DR ProteomicsDB; 66041; -. [Q68D06-1]
DR ProteomicsDB; 66042; -. [Q68D06-2]
DR Antibodypedia; 15604; 68 antibodies from 16 providers.
DR DNASU; 146857; -.
DR Ensembl; ENST00000285013.11; ENSP00000285013.6; ENSG00000154760.14. [Q68D06-1]
DR Ensembl; ENST00000526861.5; ENSP00000434439.1; ENSG00000154760.14. [Q68D06-1]
DR Ensembl; ENST00000533791.5; ENSP00000467426.1; ENSG00000154760.14. [Q68D06-1]
DR Ensembl; ENST00000534689.5; ENSP00000435442.1; ENSG00000154760.14. [Q68D06-2]
DR Ensembl; ENST00000542635.5; ENSP00000444016.1; ENSG00000154760.14. [Q68D06-1]
DR GeneID; 146857; -.
DR KEGG; hsa:146857; -.
DR MANE-Select; ENST00000285013.11; ENSP00000285013.6; NM_144682.6; NP_653283.3.
DR UCSC; uc002hjk.2; human. [Q68D06-1]
DR CTD; 146857; -.
DR DisGeNET; 146857; -.
DR GeneCards; SLFN13; -.
DR HGNC; HGNC:26481; SLFN13.
DR HPA; ENSG00000154760; Tissue enhanced (bone marrow, salivary gland).
DR MIM; 614957; gene.
DR neXtProt; NX_Q68D06; -.
DR OpenTargets; ENSG00000154760; -.
DR PharmGKB; PA144596360; -.
DR VEuPathDB; HostDB:ENSG00000154760; -.
DR eggNOG; ENOG502QWKG; Eukaryota.
DR GeneTree; ENSGT00410000025651; -.
DR HOGENOM; CLU_007071_0_0_1; -.
DR InParanoid; Q68D06; -.
DR OMA; TKQQGSC; -.
DR OrthoDB; 211385at2759; -.
DR PhylomeDB; Q68D06; -.
DR TreeFam; TF337168; -.
DR PathwayCommons; Q68D06; -.
DR BioGRID-ORCS; 146857; 8 hits in 1070 CRISPR screens.
DR ChiTaRS; SLFN13; human.
DR GenomeRNAi; 146857; -.
DR Pharos; Q68D06; Tdark.
DR PRO; PR:Q68D06; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q68D06; protein.
DR Bgee; ENSG00000154760; Expressed in right uterine tube and 115 other tissues.
DR ExpressionAtlas; Q68D06; baseline and differential.
DR Genevisible; Q68D06; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004521; F:endoribonuclease activity; IDA:UniProtKB.
DR GO; GO:0000049; F:tRNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; ISS:UniProtKB.
DR GO; GO:0051607; P:defense response to virus; IBA:GO_Central.
DR GO; GO:0016075; P:rRNA catabolic process; IDA:UniProtKB.
DR GO; GO:0016078; P:tRNA catabolic process; IDA:UniProtKB.
DR Gene3D; 3.30.950.30; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR029684; Schlafen.
DR InterPro; IPR007421; Schlafen_AlbA_2_dom.
DR InterPro; IPR038461; Schlafen_AlbA_2_dom_sf.
DR InterPro; IPR029679; SLFN13.
DR InterPro; IPR018647; SLFN_3-like_DNA/RNA_helicase.
DR PANTHER; PTHR12155; PTHR12155; 1.
DR PANTHER; PTHR12155:SF21; PTHR12155:SF21; 1.
DR Pfam; PF04326; AlbA_2; 1.
DR Pfam; PF09848; DUF2075; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Cytoplasm; Endonuclease; Hydrolase;
KW Magnesium; Metal-binding; Nuclease; Nucleotide-binding; Reference proteome;
KW Zinc.
FT CHAIN 1..897
FT /note="Schlafen family member 13"
FT /id="PRO_0000282985"
FT REGION 2..355
FT /note="N'-domain region"
FT /evidence="ECO:0000250|UniProtKB:Q5U311"
FT ACT_SITE 208
FT /evidence="ECO:0000269|PubMed:29563550"
FT ACT_SITE 213
FT /evidence="ECO:0000269|PubMed:29563550"
FT BINDING 284
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q5U311"
FT BINDING 286
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q5U311"
FT BINDING 321
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q5U311"
FT BINDING 599..606
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 9..326
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_024273"
FT VARIANT 4
FT /note="N -> S (in dbSNP:rs12943866)"
FT /id="VAR_031449"
FT VARIANT 50
FT /note="A -> T (in dbSNP:rs7216628)"
FT /id="VAR_031450"
FT VARIANT 433
FT /note="P -> L (in dbSNP:rs16970912)"
FT /id="VAR_053878"
FT VARIANT 642
FT /note="D -> V (in dbSNP:rs11657183)"
FT /id="VAR_053879"
FT VARIANT 652
FT /note="E -> K (in dbSNP:rs3744371)"
FT /id="VAR_031451"
FT MUTAGEN 208
FT /note="E->A: Abolished endoribonuclease activity."
FT /evidence="ECO:0000269|PubMed:29563550"
FT MUTAGEN 213
FT /note="E->A: Abolished endoribonuclease activity."
FT /evidence="ECO:0000269|PubMed:29563550"
FT MUTAGEN 251
FT /note="D->A: Reduced endoribonuclease activity."
FT /evidence="ECO:0000269|PubMed:29563550"
SQ SEQUENCE 897 AA; 102045 MW; 2D516123F44121D0 CRC64;
MEANHCSLGV YPSYPDLVID VGEVTLGEEN RKKLQKTQRD QERARVIRAA CALLNSGGGV
IQMEMANRDE RPTEMGLDLE ESLRKLIQYP YLQAFFETKQ HGRCFYIFVK SWSGDPFLKD
GSFNSRICSL SSSLYCRSGT SVLHMNSRQA FDFLKTKERQ SKYNLINEGS PPSKIMKAVY
QNISESNPAY EVFQTDTIEY GEILSFPESP SIEFKQFSTK HIQQYVENII PEYISAFANT
EGGYLFIGVD DKSRKVLGCA KEQVDPDSLK NVIARAISKL PIVHFCSSKP RVEYSTKIVE
VFCGKELYGY LCVIKVKAFC CVVFSEAPKS WMVREKYIRP LTTEEWVEKM MDADPEFPPD
FAEAFESQLS LSDSPSLCRP VYSKKGLEHK ADLQQHLFPV PPGHLECTPE SLWKELSLQH
EGLKELIHKQ MRPFSQGIVI LSRSWAVDLN LQEKPGVICD ALLIAQNSTP ILYTILREQD
AEGQDYCTRT AFTLKQKLVN MGGYTGKVCV RAKVLCLSPE SSAEALEAAV SPMDYPASYS
LAGTQHMEAL LQSLVIVLLG FRSLLSDQLG CEVLNLLTAQ QYEIFSRSLR KNRELFVHGL
PGSGKTIMAM KIMEKIRNVF HCEAHRILYV CENQPLRNFI SDRNICRAET RETFLREKFE
HIQHIVIDEA QNFRTEDGDW YRKAKTITQR EKDCPGVLWI FLDYFQTSHL GHSGLPPLSA
QYPREELTRV VRNADEIAEY IQQEMQLIIE NPPINIPHGY LAILSEAKWV PGVPGNTKII
KNFTLEQIVT YVADTCRCFF ERGYSPKDVA VLVSTVTEVE QYQSKLLKAM RKKMVVQLSD
ACDMLGVHIV LDSVRRFSGL ERSIVFGIHP RTADPAILPN ILICLASRAK QHLYIFL
