SLP1_DROME
ID SLP1_DROME Reviewed; 322 AA.
AC P32030; Q9VQV4;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Fork head domain transcription factor slp1;
DE AltName: Full=Sloppy paired locus protein 1;
GN Name=slp1; Synonyms=FD6; ORFNames=CG16738;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Canton-S, and Oregon-R;
RX PubMed=1317319; DOI=10.1101/gad.6.6.1030;
RA Grossniklaus U., Pearson R.K., Gehring W.J.;
RT "The Drosophila sloppy paired locus encodes two proteins involved in
RT segmentation that show homology to mammalian transcription factors.";
RL Genes Dev. 6:1030-1051(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Transcription factor involved in segmentation. Required for
CC the formation of the mandibular lobe. Different levels of slp activity
CC seem to be required in different segments.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in the posterior half of each parasegment
CC just anterior to the parasegmental boundary.
CC -!- DEVELOPMENTAL STAGE: Present at 0-3 hours of embryogenesis. Maximal
CC expression at 3-6 hours. Strong re-expression in first-instar larvae.
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DR EMBL; X66095; CAA46889.1; -; Genomic_DNA.
DR EMBL; X66096; CAA46890.1; -; mRNA.
DR EMBL; AE014134; AAF51058.1; -; Genomic_DNA.
DR RefSeq; NP_476730.1; NM_057382.3.
DR AlphaFoldDB; P32030; -.
DR SMR; P32030; -.
DR BioGRID; 59810; 10.
DR IntAct; P32030; 4.
DR STRING; 7227.FBpp0077188; -.
DR PaxDb; P32030; -.
DR DNASU; 33607; -.
DR GeneID; 33607; -.
DR KEGG; dme:Dmel_CG16738; -.
DR CTD; 33607; -.
DR FlyBase; FBgn0003430; slp1.
DR VEuPathDB; VectorBase:FBgn0003430; -.
DR eggNOG; KOG2294; Eukaryota.
DR HOGENOM; CLU_809570_0_0_1; -.
DR InParanoid; P32030; -.
DR SignaLink; P32030; -.
DR BioGRID-ORCS; 33607; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 33607; -.
DR PRO; PR:P32030; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR ExpressionAtlas; P32030; baseline and differential.
DR Genevisible; P32030; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0008595; P:anterior/posterior axis specification, embryo; NAS:FlyBase.
DR GO; GO:0007350; P:blastoderm segmentation; TAS:FlyBase.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0007507; P:heart development; TAS:FlyBase.
DR GO; GO:0060914; P:heart formation; IMP:FlyBase.
DR GO; GO:0001707; P:mesoderm formation; TAS:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0007366; P:periodic partitioning by pair rule gene; IEA:UniProtKB-KW.
DR GO; GO:0010468; P:regulation of gene expression; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007380; P:specification of segmental identity, head; IMP:FlyBase.
DR CDD; cd00059; FH; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001766; Fork_head_dom.
DR InterPro; IPR018122; TF_fork_head_CS_1.
DR InterPro; IPR030456; TF_fork_head_CS_2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00250; Forkhead; 1.
DR PRINTS; PR00053; FORKHEAD.
DR SMART; SM00339; FH; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00657; FORK_HEAD_1; 1.
DR PROSITE; PS00658; FORK_HEAD_2; 1.
DR PROSITE; PS50039; FORK_HEAD_3; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Nucleus; Pair-rule protein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..322
FT /note="Fork head domain transcription factor slp1"
FT /id="PRO_0000091914"
FT DNA_BIND 119..210
FT /note="Fork-head"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT REGION 31..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 94..120
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 46..81
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 246
FT /note="P -> S (in strain: Oregon-R and Berkeley)"
FT VARIANT 303
FT /note="P -> Q (in strain: Oregon-R and Berkeley)"
SQ SEQUENCE 322 AA; 36202 MW; 24CDE9F0102024C4 CRC64;
MVKSEMEFKS NFSIDAILAK KPINTATQPI KTEPVHHHHQ YVHPYSNSDG ELSASEDFDS
PSRTSTPMSS AAESLSSQNN DKLDVEFDDE LEDQLDEDQE SEDGNPSKKQ KMTAGSDTKK
PPYSYNALIM MAIQDSPEQR LTLNGIYQYL INRFPYFKAN KRGWQNSIRH NLSLNKCFTK
IPRSYDDPGK GNYWILDPSA EEVFIGETTG KLRRKNPGAS RTRLAAYRQA IFSPMMAASP
YGAPAPSYGY PAVPFAAAAA AALYQRMNPA AYQAAYQQMQ YQQAPQAHHH QAPHPAQMQG
YPPQLNAELF QRMQFFGKFP SS
