SLP2_DROME
ID SLP2_DROME Reviewed; 451 AA.
AC P32031; Q8SZ95; Q9VQV5;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 31-JAN-2018, sequence version 3.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Fork head domain transcription factor slp2;
DE AltName: Full=Sloppy paired locus protein 2;
GN Name=slp2; ORFNames=CG2939;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Canton-S, and Oregon-R;
RX PubMed=1317319; DOI=10.1101/gad.6.6.1030;
RA Grossniklaus U., Pearson R.K., Gehring W.J.;
RT "The Drosophila sloppy paired locus encodes two proteins involved in
RT segmentation that show homology to mammalian transcription factors.";
RL Genes Dev. 6:1030-1051(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J., Frise E., Kapadia B., Park S., Wan K., Yu C.,
RA Celniker S.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcription factor involved in segmentation. May function
CC primarily as a segment polarity gene. Different levels of slp activity
CC seem to be required in different segments.
CC {ECO:0000269|PubMed:1317319}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00089}.
CC -!- TISSUE SPECIFICITY: Expressed in the posterior half of each parasegment
CC just anterior to the parasegmental boundary.
CC {ECO:0000269|PubMed:1317319}.
CC -!- DEVELOPMENTAL STAGE: Expression at 3-6 hours of embryogenesis. Strong
CC re-expression in first-instar larvae. {ECO:0000269|PubMed:1317319}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA46891.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA46892.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X66098; CAA46892.1; ALT_FRAME; Genomic_DNA.
DR EMBL; X66097; CAA46891.1; ALT_FRAME; mRNA.
DR EMBL; AE014134; AAF51057.1; -; Genomic_DNA.
DR EMBL; AY071031; AAL48653.1; -; mRNA.
DR EMBL; BT030401; ABO52820.1; -; mRNA.
DR PIR; S23055; S23055.
DR PIR; S23056; S23056.
DR RefSeq; NP_476834.1; NM_057486.4.
DR AlphaFoldDB; P32031; -.
DR SMR; P32031; -.
DR BioGRID; 59811; 7.
DR IntAct; P32031; 1.
DR STRING; 7227.FBpp0077189; -.
DR PaxDb; P32031; -.
DR DNASU; 33608; -.
DR EnsemblMetazoa; FBtr0077500; FBpp0077189; FBgn0004567.
DR GeneID; 33608; -.
DR KEGG; dme:Dmel_CG2939; -.
DR CTD; 33608; -.
DR FlyBase; FBgn0004567; slp2.
DR VEuPathDB; VectorBase:FBgn0004567; -.
DR eggNOG; KOG2294; Eukaryota.
DR GeneTree; ENSGT00940000165356; -.
DR InParanoid; P32031; -.
DR OMA; YPQFGQF; -.
DR OrthoDB; 1270467at2759; -.
DR Reactome; R-DME-9617828; FOXO-mediated transcription of cell cycle genes.
DR SignaLink; P32031; -.
DR BioGRID-ORCS; 33608; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33608; -.
DR PRO; PR:P32031; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0004567; Expressed in ectoderm anlage and 38 other tissues.
DR ExpressionAtlas; P32031; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007367; P:segment polarity determination; IEA:UniProtKB-KW.
DR CDD; cd00059; FH; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001766; Fork_head_dom.
DR InterPro; IPR018122; TF_fork_head_CS_1.
DR InterPro; IPR030456; TF_fork_head_CS_2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00250; Forkhead; 1.
DR PRINTS; PR00053; FORKHEAD.
DR SMART; SM00339; FH; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00657; FORK_HEAD_1; 1.
DR PROSITE; PS00658; FORK_HEAD_2; 1.
DR PROSITE; PS50039; FORK_HEAD_3; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Segmentation polarity protein; Transcription; Transcription regulation.
FT CHAIN 1..451
FT /note="Fork head domain transcription factor slp2"
FT /id="PRO_0000091915"
FT DNA_BIND 180..274
FT /note="Fork-head"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT REGION 22..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 73..181
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 330..369
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 402..451
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 91..108
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 137..157
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 158..180
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 334..368
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 134
FT /note="P -> H (in Ref. 4; AAL48653 and 5; ABO52820)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 451 AA; 49238 MW; 170C7338503B86B5 CRC64;
MVKIEEGLPS SEISAHSLHF QHHHHPLPPT THHSALQSPH PVGLNLTNLM KMARTPHLKS
SFSINSILPE TVEHHDEDEE EDVEKKSPAK FPPNHNNNNL NTTNWGSPED HEAESDPESD
LDVTSMSPAP VANPNESDPD EVDEEFVEED IECDGETTDG DAENKSNDGK PVKDKKGNEK
PPYSYNALIM MAIRQSSEKR LTLNGIYEYI MTNHPYYRDN KQGWQNSIRH NLSLNKCFVK
VPRHYDDPGK GNYWMLDPSA EDVFIGGSTG KLRRRTTAAS RSRLAAFKRS LIGPMFPGLA
AYPQFGQFLT YPPTAPSLLA SMYQRYNPFA PKGGPGHPGL PPGLPGLPGP PGPQGPPGPP
PPPFVAPPTS SELYQRLQYQ QLLHQHAAAA ALAAHQRQLS VAAASAASQP PPTHHHPHLA
VGQAPLSPGG DSPGPSPQPL HKPVTVVSRN S
