SLT2_CHLRE
ID SLT2_CHLRE Reviewed; 883 AA.
AC D2K6F1; A8IHV5;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 09-FEB-2010, sequence version 1.
DT 25-MAY-2022, entry version 52.
DE RecName: Full=Sodium/sulfate cotransporter 2;
DE AltName: Full=SAC1-like transporter 2;
DE Short=CrSLT2;
GN Name=SLT2; ORFNames=CHLREDRAFT_205501;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION BY SULFUR, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=20498339; DOI=10.1104/pp.110.157875;
RA Pootakham W., Gonzalez-Ballester D., Grossman A.R.;
RT "Identification and regulation of plasma membrane sulfate transporters in
RT Chlamydomonas.";
RL Plant Physiol. 153:1653-1668(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
CC -!- FUNCTION: Na(+)/sulfate cotransporter with a probable high-affinity for
CC sulfate and a proteasome dependent turnover.
CC {ECO:0000269|PubMed:20498339}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20498339};
CC Multi-pass membrane protein {ECO:0000269|PubMed:20498339}.
CC -!- INDUCTION: Up-regulated by sulfur deprivation.
CC {ECO:0000269|PubMed:20498339}.
CC -!- DISRUPTION PHENOTYPE: No effect on sulfate uptake, due to the
CC redundancy with SLT1 and SULTR2. Slt1 and slt2, as well as slt1 and
CC sultr2 double mutants show a 50% decline in uptake while the slt1, slt2
CC and sultr2 triple mutant exhibits no increase in sulfate uptake
CC capacity when deprived of sulfur. Modified regulation of the SLT3 gene
CC that becomes up-regulated by sulfur deprivation instead of down-
CC regulated as in wild-type. {ECO:0000269|PubMed:20498339}.
CC -!- SIMILARITY: Belongs to the divalent anion:Na+ symporter (DASS)
CC superfamily. Na+/sulfate symporter (TC 2.A.47.4) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EDP05750.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; GU181276; ACZ63169.1; -; mRNA.
DR EMBL; DS496117; EDP05750.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; D2K6F1; -.
DR STRING; 3055.EDP05750; -.
DR TCDB; 2.A.47.4.4; the divalent anion:na(+) symporter (dass) family.
DR PaxDb; D2K6F1; -.
DR EnsemblPlants; PNW77626; PNW77626; CHLRE_10g445000v5.
DR EnsemblPlants; PNW77627; PNW77627; CHLRE_10g445000v5.
DR Gramene; PNW77626; PNW77626; CHLRE_10g445000v5.
DR Gramene; PNW77627; PNW77627; CHLRE_10g445000v5.
DR eggNOG; ENOG502QPZM; Eukaryota.
DR InParanoid; D2K6F1; -.
DR OMA; AVQWDVI; -.
DR OrthoDB; 817305at2759; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015116; F:sulfate transmembrane transporter activity; IMP:UniProtKB.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006813; P:potassium ion transport; IEA:InterPro.
DR Gene3D; 3.30.70.1450; -; 4.
DR InterPro; IPR004680; Cit_transptr-like_dom.
DR InterPro; IPR006037; RCK_C.
DR InterPro; IPR036721; RCK_C_sf.
DR Pfam; PF03600; CitMHS; 1.
DR Pfam; PF02080; TrkA_C; 3.
DR SUPFAM; SSF116726; SSF116726; 4.
DR PROSITE; PS51202; RCK_C; 4.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Repeat; Stress response; Sulfate transport;
KW Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..883
FT /note="Sodium/sulfate cotransporter 2"
FT /id="PRO_0000417125"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 600..620
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 624..644
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 657..677
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 693..713
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 774..794
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 802..822
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 211..295
FT /note="RCK C-terminal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
FT DOMAIN 317..401
FT /note="RCK C-terminal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
FT DOMAIN 406..491
FT /note="RCK C-terminal 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
FT DOMAIN 497..583
FT /note="RCK C-terminal 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
FT REGION 857..883
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 883 AA; 95220 MW; C47547D11B5A5E6C CRC64;
MGFGWQGSVS IAFTALAFVV MAADWVGPDV TFTVLLAFLT AFDGQIVTVA KAAAGYGNTG
LLTVIFLYWV AEGITQTGGL ELIMNFVLGR SRSVHWALAR SMFPVMCLSA FLNNTPCVTF
MIPILISWGR RCGVPIKKLL IPLSYASVLG GTCTSIGTST NLVIVGLQDA RYTKAKQLDQ
AKFQIFDIAP YGVPYALWGF VFILLTQAFL LPGNSSRYAK DLLIAVRVLP SSSVAKKKLK
DSGLLQQSGF SVSGIYRDGK YLSKPDPNWV LEPNDILYAA GEFDVVEFVG EEFGLGLVNA
DAETSAERPF TTGEESVFTP TGGAPYQKLV QATIAPTSDL IGRTVREVSW QGRFGLIPVA
IQRGNGREDG RLNDVVLAAG DVLILDTTPF YDEEREDSKN NFAGKVRAVK DGAAKEFVVG
VKVKKSSEVV NKTVSAAGLR GIPGLFVLSV DRADGSSVEA SDYLYKIQPD DTIWIATDIG
AVGFLAKFPG LELVQQEQVD KTGTSILYRH LVQAAVSHKG PIVGKTVRDV RFRTLYNAAV
VAVHREGARV PLKVQDIVLQ GGDVLLISCH TNWADEHRHD KSFVLLQPVP DSSPPKRSRM
VIGVLLATGM VLTQIVGGLK SREYIHLWPA AVLTSALMLL TGCMNADQAR KAIYWDVYLT
IAAAFGVSAA LEGTGVAASF ANGIISIGKN LHSDGAALIA IYIATAMLSE LLTNNAAGAI
MYPIAAIAGD ALKISPKETS VAIMLGASAG FINPFSYQCN LMVYAAGNYS VREFAIIGAP
FQIWLMIVAG FILCYMKEWH QVWIVSWICT AGIVLLPALY FLLPTKVQLR IDAFFDRVAQ
TLNPKLIIER RNSIRRQASR TGSDGTGSSD SPRALGVPKV ITA
