ID   SLU7_DANRE              Reviewed;         571 AA.
AC   Q5U3F2;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Pre-mRNA-splicing factor SLU7;
GN   Name=slu7; ORFNames=zgc:103640;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Larva;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for pre-mRNA splicing as component of the
CC       spliceosome. Participates in the second catalytic step of pre-mRNA
CC       splicing, when the free hydroxyl group of exon I attacks the 3'-splice
CC       site to generate spliced mRNA and the excised lariat intron. Required
CC       for holding exon 1 properly in the spliceosome and for correct AG
CC       identification when more than one possible AG exists in 3'-splicing
CC       site region. May be involved in the activation of proximal AG. Probably
CC       also involved in alternative splicing regulation.
CC       {ECO:0000250|UniProtKB:O95391}.
CC   -!- SUBUNIT: Component of pre-catalytic, catalytic and post-catalytic
CC       spliceosomes. Associates with the spliceosome prior to recognition of
CC       the 3'-splice site for step II, probably during catalysis of step I.
CC       {ECO:0000250|UniProtKB:O95391}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O95391}. Nucleus
CC       speckle {ECO:0000250|UniProtKB:O95391}. Cytoplasm
CC       {ECO:0000250|UniProtKB:O95391}. Note=Predominantly nuclear.
CC       {ECO:0000250|UniProtKB:O95391}.
CC   -!- SIMILARITY: Belongs to the SLU7 family. {ECO:0000305}.
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DR   EMBL; BC085570; AAH85570.1; -; mRNA.
DR   RefSeq; NP_001007368.1; NM_001007367.1.
DR   AlphaFoldDB; Q5U3F2; -.
DR   SMR; Q5U3F2; -.
DR   STRING; 7955.ENSDARP00000112935; -.
DR   PaxDb; Q5U3F2; -.
DR   GeneID; 492495; -.
DR   KEGG; dre:492495; -.
DR   CTD; 10569; -.
DR   ZFIN; ZDB-GENE-041114-62; slu7.
DR   eggNOG; KOG2560; Eukaryota.
DR   InParanoid; Q5U3F2; -.
DR   PhylomeDB; Q5U3F2; -.
DR   Reactome; R-DRE-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR   Reactome; R-DRE-72187; mRNA 3'-end processing.
DR   Reactome; R-DRE-73856; RNA Polymerase II Transcription Termination.
DR   PRO; PR:Q5U3F2; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR   GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030628; F:pre-mRNA 3'-splice site binding; IEA:InterPro.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IEA:InterPro.
DR   GO; GO:0008380; P:RNA splicing; IBA:GO_Central.
DR   InterPro; IPR021715; Slu7_dom.
DR   InterPro; IPR039974; Splicing_factor_SLU7.
DR   PANTHER; PTHR12942; PTHR12942; 1.
DR   Pfam; PF11708; Slu7; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Metal-binding; mRNA processing; mRNA splicing; Nucleus;
KW   Reference proteome; Spliceosome; Zinc; Zinc-finger.
FT   CHAIN           1..571
FT                   /note="Pre-mRNA-splicing factor SLU7"
FT                   /id="PRO_0000289199"
FT   ZN_FING         111..128
FT                   /note="CCHC-type"
FT   REGION          1..91
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          199..235
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          462..571
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        10..53
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        492..562
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   571 AA;  66241 MW;  7BF6A028E69621B0 CRC64;
     MAAEAGKASE GMVDLEEPKK MTREDWRKKK ELEEQRKLGN APAEVDEEGK DINPHIPQYI
     SSVPWYIDPS KRPTLKHQRP QEENQSKFAP IGDWYKRGVQ EKSVNTKYRK GACENCGALT
     HKKKDCLERP RKVGAKFSGT GIAPDEHQQV QLSMDYDGKR DRWNGYDPDE HMRIVEEYSK
     VDLAKRTLKA QKLQEELASG KLMDQANSRK HEEAVQDHSS EDEDEDKYVD DFDMPGQNFD
     SKRRITVRNL RIREDIAKYL RNLDPNSAYY DPKTRAMREN PYSNTGKNPE EVGYAGDNFV
     RYSGDTISMA QTQLFAWEAY EKGSEVHLQA DPTKLELLHQ SYKVKKDDFK EKQKETILEK
     YGGSEHLDAP PRELLLAQTE EYVEYSRHGA VLKGQEKAVA QSKYEEDVLN NNHTCIWGSY
     WKDGYWGYKC CHSMVKQSYC TGEAGKKVVS NSCTPFEEDV EEAQTSEEPK TLLQMHQEKL
     KDKKKKKKSK KHRDSDSSDE EDEAKKKEKL KKALSAEEQR LKQVAELMQV DERKRPYNSL
     MEVREPTEEE MEAFRMKRCR PDDPMASFLG Q