ID   SLX1_BOVIN              Reviewed;         267 AA.
AC   Q32PI0;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Structure-specific endonuclease subunit SLX1 {ECO:0000255|HAMAP-Rule:MF_03100};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_03100};
DE   AltName: Full=GIY-YIG domain-containing protein 1 {ECO:0000255|HAMAP-Rule:MF_03100};
GN   Name=SLX1A {ECO:0000255|HAMAP-Rule:MF_03100};
GN   Synonyms=GIYD1 {ECO:0000255|HAMAP-Rule:MF_03100}, SLX1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Hypothalamus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalytic subunit of the SLX1-SLX4 structure-specific
CC       endonuclease that resolves DNA secondary structures generated during
CC       DNA repair and recombination. Has endonuclease activity towards
CC       branched DNA substrates, introducing single-strand cuts in duplex DNA
CC       close to junctions with ss-DNA. Has a preference for 5'-flap
CC       structures, and promotes symmetrical cleavage of static and migrating
CC       Holliday junctions (HJs). Resolves HJs by generating two pairs of
CC       ligatable, nicked duplex products. {ECO:0000255|HAMAP-Rule:MF_03100}.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_03100};
CC   -!- SUBUNIT: Forms a heterodimer with SLX4. {ECO:0000255|HAMAP-
CC       Rule:MF_03100}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03100}.
CC   -!- SIMILARITY: Belongs to the SLX1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03100}.
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DR   EMBL; BC108108; AAI08109.1; -; mRNA.
DR   RefSeq; NP_001032533.1; NM_001037456.2.
DR   AlphaFoldDB; Q32PI0; -.
DR   SMR; Q32PI0; -.
DR   STRING; 9913.ENSBTAP00000011384; -.
DR   PaxDb; Q32PI0; -.
DR   PRIDE; Q32PI0; -.
DR   Ensembl; ENSBTAT00000011384; ENSBTAP00000011384; ENSBTAG00000008633.
DR   GeneID; 510858; -.
DR   KEGG; bta:510858; -.
DR   CTD; 548593; -.
DR   VEuPathDB; HostDB:ENSBTAG00000008633; -.
DR   eggNOG; KOG3005; Eukaryota.
DR   GeneTree; ENSGT00390000013368; -.
DR   HOGENOM; CLU_030739_0_0_1; -.
DR   InParanoid; Q32PI0; -.
DR   OrthoDB; 844266at2759; -.
DR   TreeFam; TF352344; -.
DR   Reactome; R-BTA-5693568; Resolution of D-loop Structures through Holliday Junction Intermediates.
DR   Reactome; R-BTA-6783310; Fanconi Anemia Pathway.
DR   Proteomes; UP000009136; Chromosome 25.
DR   Bgee; ENSBTAG00000008633; Expressed in neutrophil and 105 other tissues.
DR   ExpressionAtlas; Q32PI0; baseline and differential.
DR   GO; GO:0033557; C:Slx1-Slx4 complex; IBA:GO_Central.
DR   GO; GO:0017108; F:5'-flap endonuclease activity; IBA:GO_Central.
DR   GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR   Gene3D; 3.30.40.10; -; 1.
DR   Gene3D; 3.40.1440.10; -; 1.
DR   HAMAP; MF_03100; Endonuc_su_Slx1; 1.
DR   InterPro; IPR000305; GIY-YIG_endonuc.
DR   InterPro; IPR035901; GIY-YIG_endonuc_sf.
DR   InterPro; IPR027520; Slx1.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   Pfam; PF01541; GIY-YIG; 1.
DR   SMART; SM00465; GIYc; 1.
DR   SUPFAM; SSF82771; SSF82771; 1.
DR   PROSITE; PS50164; GIY_YIG; 1.
PE   2: Evidence at transcript level;
KW   DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase;
KW   Metal-binding; Nuclease; Nucleus; Reference proteome; Zinc; Zinc-finger.
FT   CHAIN           1..267
FT                   /note="Structure-specific endonuclease subunit SLX1"
FT                   /id="PRO_0000332119"
FT   DOMAIN          5..90
FT                   /note="GIY-YIG"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03100"
FT   ZN_FING         178..230
FT                   /note="SLX1-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03100"
FT   REGION          139..169
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        139..158
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   267 AA;  30202 MW;  B0555BA5CB9806EE CRC64;
     MGPGCFFGVY LLYCLNPRHR GRVYVGFTVN PARRVQQHNG GRKKGGAWRT SGRGPWEMVL
     IVHGFPSAVA ALRFEWAWQH PQASRRLTHV GRRLRGEATF AFHLRVLAHM LRAPPWVRLP
     LTLRWLRADF RQDLCPPPPP HMPLAFGPPP PRAPPPRSQA GPSDDMGFEP QQDVDVRCTL
     CARALQDEES PLCCPHPGCS LRAHVICLAE EFLQEEPGQL LPLEGACPGC KNSLLWGDLI
     WLCRMGTEED EEDSELEEEH WTDMLKT