SLX4_DROME
ID SLX4_DROME Reviewed; 1145 AA.
AC Q9VS48;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Structure-specific endonuclease subunit SLX4;
DE AltName: Full=Mutagen-sensitive protein 312;
GN Name=mus312; Synonyms=SLX4; ORFNames=CG8601;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP FUNCTION, AND INTERACTION WITH MEI-9.
RX PubMed=12504024; DOI=10.1016/s1097-2765(02)00782-7;
RA Yildiz O., Majumder S., Kramer B., Sekelsky J.J.;
RT "Drosophila MUS312 interacts with the nucleotide excision repair
RT endonuclease MEI-9 to generate meiotic crossovers.";
RL Mol. Cell 10:1503-1509(2002).
RN [4]
RP FUNCTION, AND INTERACTION WITH SLX1.
RX PubMed=19596236; DOI=10.1016/j.cell.2009.06.029;
RA Fekairi S., Scaglione S., Chahwan C., Taylor E.R., Tissier A., Coulon S.,
RA Dong M.-Q., Ruse C., Yates J.R. III, Russell P., Fuchs R.P., McGowan C.H.,
RA Gaillard P.-H.L.;
RT "Human SLX4 is a Holliday junction resolvase subunit that binds multiple
RT DNA repair/recombination endonucleases.";
RL Cell 138:78-89(2009).
RN [5]
RP FUNCTION, AND INTERACTION WITH MEI-9 AND SLX1.
RX PubMed=19595722; DOI=10.1016/j.molcel.2009.06.019;
RA Andersen S.L., Bergstralh D.T., Kohl K.P., LaRocque J.R., Moore C.B.,
RA Sekelsky J.;
RT "Drosophila MUS312 and the vertebrate ortholog BTBD12 interact with DNA
RT structure-specific endonucleases in DNA repair and recombination.";
RL Mol. Cell 35:128-135(2009).
CC -!- FUNCTION: Regulatory subunit that interacts with and increases the
CC activity of different structure-specific endonucleases. Has several
CC distinct roles in protecting genome stability by resolving diverse
CC forms of deleterious DNA structures originating from replication and
CC recombination intermediates and from DNA damage (By similarity).
CC Component of the SLX1-SLX4 structure-specific endonuclease that
CC resolves DNA secondary structures generated during DNA repair and
CC recombination. Has endonuclease activity towards branched DNA
CC substrates, introducing single-strand cuts in duplex DNA close to
CC junctions with ss-DNA (By similarity). Interacts with the structure-
CC specific MEI-9-ERCC1 endonuclease to generate meiotic crossovers.
CC {ECO:0000250, ECO:0000269|PubMed:12504024, ECO:0000269|PubMed:19595722,
CC ECO:0000269|PubMed:19596236}.
CC -!- SUBUNIT: Forms a heterodimer with SLX1. Interacts with mei-9; catalytic
CC subunit of the MEI-9-ERCC1 endonuclease. {ECO:0000269|PubMed:12504024,
CC ECO:0000269|PubMed:19595722, ECO:0000269|PubMed:19596236}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SLX4 family. {ECO:0000305}.
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DR EMBL; AE014296; AAF50581.2; -; Genomic_DNA.
DR RefSeq; NP_648104.2; NM_139847.4.
DR AlphaFoldDB; Q9VS48; -.
DR BioGRID; 64249; 7.
DR STRING; 7227.FBpp0076550; -.
DR PaxDb; Q9VS48; -.
DR EnsemblMetazoa; FBtr0076839; FBpp0076550; FBgn0002909.
DR GeneID; 38809; -.
DR KEGG; dme:Dmel_CG8601; -.
DR UCSC; CG8601-RA; d. melanogaster.
DR CTD; 38809; -.
DR FlyBase; FBgn0002909; mus312.
DR VEuPathDB; VectorBase:FBgn0002909; -.
DR eggNOG; ENOG502S74K; Eukaryota.
DR HOGENOM; CLU_309563_0_0_1; -.
DR InParanoid; Q9VS48; -.
DR PhylomeDB; Q9VS48; -.
DR Reactome; R-DME-5693568; Resolution of D-loop Structures through Holliday Junction Intermediates.
DR BioGRID-ORCS; 38809; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38809; -.
DR PRO; PR:Q9VS48; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0002909; Expressed in ovary and 10 other tissues.
DR Genevisible; Q9VS48; DM.
DR GO; GO:0032991; C:protein-containing complex; IDA:FlyBase.
DR GO; GO:0033557; C:Slx1-Slx4 complex; IDA:UniProtKB.
DR GO; GO:0019899; F:enzyme binding; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IGI:FlyBase.
DR GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR GO; GO:0035825; P:homologous recombination; IGI:FlyBase.
DR GO; GO:0036297; P:interstrand cross-link repair; IBA:GO_Central.
DR GO; GO:0045132; P:meiotic chromosome segregation; IMP:FlyBase.
DR GO; GO:0000706; P:meiotic DNA double-strand break processing; IBA:GO_Central.
DR GO; GO:0030716; P:oocyte fate determination; IMP:FlyBase.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:FlyBase.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IMP:FlyBase.
DR InterPro; IPR018574; Structure-sp_endonuc_su_Slx4.
DR Pfam; PF09494; Slx4; 1.
PE 1: Evidence at protein level;
KW Coiled coil; DNA damage; DNA recombination; DNA repair; Nucleus;
KW Reference proteome.
FT CHAIN 1..1145
FT /note="Structure-specific endonuclease subunit SLX4"
FT /id="PRO_0000383568"
FT REGION 48..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 382..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 613..634
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 666..689
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 321..372
FT /evidence="ECO:0000255"
FT COMPBIAS 84..108
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 408..433
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1145 AA; 128216 MW; 600A1BA5BEB87C6C CRC64;
MDRKTRRANF KNLQPRLTRS TKAAADTLSL SNFFATPENA EEVPSCLADI PVQPDPPAPK
IKPIRTSNVF EKPGPKPKKA PKLKASTVST SSGARRGRGK SKQQPSISNF LRNEQIFAEV
TAQHCMADNF SADDIEMALA LSKSESEKHG RLRLHDDDDA VVDLLDDEEK STERIRHKLQ
KYGFRTAAKE DYKSLAVLPV VASKGGRRGK WANRFTSLTL RNPDVQQKKL EEKVSALLGQ
EMRGKEPNGE DCCLTPYTVI TAALKELRAE GESRILREPS EGPIDDLESY YVTDLFEVSR
TPAHHLLKNW AAIQGRDFSP ERETQKCRQL RQQHELVYAE LERYYGDPQK LEEEVMQELD
ELEKLVADNM IEDDSVVINI VEAESSSTGS SPSKEPPDKR PKMTMEDKEN LQPTTSKASL
TVPAQSTRCT SPDLFADSED EPDIVTTAIS NEPEDVRNLS MKVYKNVSIS ERSSSVAEIE
IVSSNDEPSQ ITTYEVFSSD EVKTVSNATQ EKISFDDDFI DLTQEFDMVE FNESKPRTPN
LIASSETDSN VDNLNGDTIL DFSSQEVSCP ISKELYAKYA KVETFPVWNA AAEEEDKIEF
SLSMERDLKS SEQSSHQLGI LTTPNDTEHS SSFSELNFSR NSLRRSVSLS TDLSFKSPLN
WKMNSSAEKR VSPAASPYKQ SDASVDLTQN SDDENDAILL SDEEINYSIW KGNKTVKDLD
IGDDSSDSCF ASPVTKKRAI PQFQTEEDLD AFLMDFSTDG NGSQGSHSPN KSALSKERAE
FGILDAAPSQ PFSLSELQSH ADKEKSSDNE INWAEASFLD APAKPLSRRS SHKFNELLAK
ISKPAHNSGD DFDEFDQMVF QSTKEAASSA GETSDMPQGL DLLLKGEIKT TAIPETRAPG
NQTPIEPQQV DVDGNVYSVR VCHTPKPDFA TLPESEILQQ LYKYGIKPLK RKQAVKMLEF
IYNQTHPIMK TAAVQDLPAR SEPIVRSKST PVIMERPHSQ VAKSTSKTLK AIGTKKDLTF
NDATGEELLR FSQSLAPSLC DDFETFVMQT NVTKKTPQPL VPLHIAWHNL ICANPQLHES
VLTYEPIDLQ AVYLHLKHMG HRYDPKDLKT FFDRRCIIFR YELGAPGKQA ERHVRRHTKK
PSKRK
