ID   SLX4_KLULA              Reviewed;         692 AA.
AC   Q6CQJ9;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Structure-specific endonuclease subunit SLX4 {ECO:0000255|HAMAP-Rule:MF_03110};
GN   Name=SLX4 {ECO:0000255|HAMAP-Rule:MF_03110};
GN   OrderedLocusNames=KLLA0D16544g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Regulatory subunit of the SLX1-SLX4 structure-specific
CC       endonuclease that resolves DNA secondary structures generated during
CC       DNA repair and recombination. Has endonuclease activity towards
CC       branched DNA substrates, introducing single-strand cuts in duplex DNA
CC       close to junctions with ss-DNA. {ECO:0000255|HAMAP-Rule:MF_03110}.
CC   -!- SUBUNIT: Forms a heterodimer with SLX1. {ECO:0000255|HAMAP-
CC       Rule:MF_03110}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03110}.
CC   -!- PTM: Phosphorylated in response to DNA damage. {ECO:0000255|HAMAP-
CC       Rule:MF_03110}.
CC   -!- SIMILARITY: Belongs to the SLX4 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03110}.
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DR   EMBL; CR382124; CAH00886.1; -; Genomic_DNA.
DR   RefSeq; XP_453790.1; XM_453790.1.
DR   AlphaFoldDB; Q6CQJ9; -.
DR   SMR; Q6CQJ9; -.
DR   STRING; 28985.XP_453790.1; -.
DR   EnsemblFungi; CAH00886; CAH00886; KLLA0_D16544g.
DR   GeneID; 2893168; -.
DR   KEGG; kla:KLLA0_D16544g; -.
DR   eggNOG; ENOG502RYEW; Eukaryota.
DR   HOGENOM; CLU_022388_0_0_1; -.
DR   InParanoid; Q6CQJ9; -.
DR   OMA; FMNTQIQ; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0033557; C:Slx1-Slx4 complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0017108; F:5'-flap endonuclease activity; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   GO; GO:0006996; P:organelle organization; IEA:UniProt.
DR   HAMAP; MF_03110; Endonuc_su_Slx4; 1.
DR   InterPro; IPR027784; Slx4_ascomycetes.
DR   InterPro; IPR018574; Structure-sp_endonuc_su_Slx4.
DR   Pfam; PF09494; Slx4; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; Nucleus; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..692
FT                   /note="Structure-specific endonuclease subunit SLX4"
FT                   /id="PRO_0000388029"
FT   REGION          39..59
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        39..58
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   692 AA;  77654 MW;  44BFCBF64F192090 CRC64;
     MHDELEKAGR HLLMVNDHLS EDSVEQGDIC MTQVPTMFSD DEDQDEEQET EIPPEEGDDD
     ILISTQVQGA IDELNHQETV VHTFRNVLSR FALETQDQAD IPLVSSKKVP VKKVIKSSKV
     KQPTARLQKR IKSITQFNTD NWESNRVKDR AQKMISILSG KSAKVKKLVS KLDSEASADA
     ESLQDSHPTQ FAPFNESEWQ HIVGLLREKL PKVSRSELNS VQQYVYGADE QHNLWKASQL
     SPEKQISSQC SDGESETILP SLTLDNNAPV YTLSQLVEGE SSNNRCSTED GLRFKPISNP
     SSVIEQELPI SIDVPSSETD VPSQIADSCD SGSIISFEEL SFMIQDKNKA DVDYDIPCYL
     PPDSKTLPTG EKTKREYSRA NINDQLINIS QTSYDVVSSI VSPMKAQRTG TIQVPATRTT
     TFQDQQQQNQ QQASKPANVV KIYVDSNRDS NDVFASVKGE VDTQEQESLS TLTTSMLHDD
     NNEIVIDSED ERGSYSIMEV HDAPQTHLGQ FPSLRKSQSQ KLKTPVSATD SLDSQLQPAL
     LTTQNASPNT AKLRRSFKVI GLKPCKNKVQ MLQVWETLES KFPGSSDEDR SIQLKQFLTD
     LIVQNRDESA LLMEQIYTFE PIRYEQLKEW LVSLNSFTEL IDDSFIKNWA DLNGIVFRND
     TENPLLSQSQ SQSQSRLLSQ SLSQLQSPSL SP