ID   SLX4_LACTC              Reviewed;         658 AA.
AC   C5DMW3;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Structure-specific endonuclease subunit SLX4 {ECO:0000255|HAMAP-Rule:MF_03110};
GN   Name=SLX4 {ECO:0000255|HAMAP-Rule:MF_03110};
GN   OrderedLocusNames=KLTH0G12166g;
OS   Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)
OS   (Yeast) (Kluyveromyces thermotolerans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Lachancea.
OX   NCBI_TaxID=559295;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 56472 / CBS 6340 / NRRL Y-8284;
RX   PubMed=19525356; DOI=10.1101/gr.091546.109;
RG   The Genolevures Consortium;
RA   Souciet J.-L., Dujon B., Gaillardin C., Johnston M., Baret P.V.,
RA   Cliften P., Sherman D.J., Weissenbach J., Westhof E., Wincker P., Jubin C.,
RA   Poulain J., Barbe V., Segurens B., Artiguenave F., Anthouard V.,
RA   Vacherie B., Val M.-E., Fulton R.S., Minx P., Wilson R., Durrens P.,
RA   Jean G., Marck C., Martin T., Nikolski M., Rolland T., Seret M.-L.,
RA   Casaregola S., Despons L., Fairhead C., Fischer G., Lafontaine I., Leh V.,
RA   Lemaire M., de Montigny J., Neuveglise C., Thierry A., Blanc-Lenfle I.,
RA   Bleykasten C., Diffels J., Fritsch E., Frangeul L., Goeffon A.,
RA   Jauniaux N., Kachouri-Lafond R., Payen C., Potier S., Pribylova L.,
RA   Ozanne C., Richard G.-F., Sacerdot C., Straub M.-L., Talla E.;
RT   "Comparative genomics of protoploid Saccharomycetaceae.";
RL   Genome Res. 19:1696-1709(2009).
CC   -!- FUNCTION: Regulatory subunit of the SLX1-SLX4 structure-specific
CC       endonuclease that resolves DNA secondary structures generated during
CC       DNA repair and recombination. Has endonuclease activity towards
CC       branched DNA substrates, introducing single-strand cuts in duplex DNA
CC       close to junctions with ss-DNA. {ECO:0000255|HAMAP-Rule:MF_03110}.
CC   -!- SUBUNIT: Forms a heterodimer with SLX1. {ECO:0000255|HAMAP-
CC       Rule:MF_03110}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03110}.
CC   -!- PTM: Phosphorylated in response to DNA damage. {ECO:0000255|HAMAP-
CC       Rule:MF_03110}.
CC   -!- SIMILARITY: Belongs to the SLX4 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03110}.
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DR   EMBL; CU928171; CAR25124.1; -; Genomic_DNA.
DR   RefSeq; XP_002555561.1; XM_002555515.1.
DR   AlphaFoldDB; C5DMW3; -.
DR   SMR; C5DMW3; -.
DR   STRING; 559295.C5DMW3; -.
DR   PRIDE; C5DMW3; -.
DR   EnsemblFungi; CAR25124; CAR25124; KLTH0G12166g.
DR   GeneID; 8293843; -.
DR   KEGG; lth:KLTH0G12166g; -.
DR   eggNOG; ENOG502RYEW; Eukaryota.
DR   HOGENOM; CLU_022388_0_0_1; -.
DR   InParanoid; C5DMW3; -.
DR   OMA; FMNTQIQ; -.
DR   OrthoDB; 1009961at2759; -.
DR   Proteomes; UP000002036; Chromosome G.
DR   GO; GO:0033557; C:Slx1-Slx4 complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0017108; F:5'-flap endonuclease activity; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   GO; GO:0006996; P:organelle organization; IEA:UniProt.
DR   HAMAP; MF_03110; Endonuc_su_Slx4; 1.
DR   InterPro; IPR027784; Slx4_ascomycetes.
DR   InterPro; IPR018574; Structure-sp_endonuc_su_Slx4.
DR   Pfam; PF09494; Slx4; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; Nucleus; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..658
FT                   /note="Structure-specific endonuclease subunit SLX4"
FT                   /id="PRO_0000388030"
FT   REGION          17..37
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          74..123
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          327..383
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        89..117
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        361..383
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   658 AA;  73649 MW;  91B517412155B373 CRC64;
     MNFDIVQRRL SLADDVVDSD SPMQEQDELP MTQIPGDSSA NVLISTQVQS KLDEMELKKV
     MKKNLTQFAF ESLGATESAP PSRAATPPAK ATKRKKSTKA AGRTSTGTGK KRKRTPSSIK
     SITQFNTENY ESLADNRRSR HVVSLLSGKK KKIKDIIDRL QVEDASGSPG PSKSHFSTYS
     PHEWSHILQL LKSKFPKCPP GQVEAVYKYV YGGAEKDNVW YSSQMPPSDP ETQASQEFHY
     NAQQPMVFSL SQAVEEEFVD AESPALISES FGREQMKGPS FLDEQCISDT TDETGVELKI
     DEDVARAFRT RMLQPYWLRE DVKMKEQPGV SGDKLMKSSS GAARPKEQRW LHLSYNGVDD
     ATKNFPKSPT STPEPRSGPQ VLQRHTYRTP SGLAGRDQLI DLTQCSFNAV KSLISPLKSE
     VQVPATRTTT WNDKNHSRPQ NYGALHQKVQ LRLCGGIADS EIYSTLRSRQ VVWRLQDFDL
     QDSEEETKYQ LLELDFETVD SKVDLSTAPH HRLSVIKSPT SIASPGDLCD FEVSVKRNAS
     VPPSLPLSAK NLRESLRAIG LKPARTRSEM AEQMGCASQH LIGENAQEQR QGLFDQFTHL
     VEQSPSLLEK VYTFEPLVLS ELIDFLVQKD PFIDKIDDST IKQWADQMGI CLRSAAED