ID   SLX4_PICST              Reviewed;         782 AA.
AC   A3GGG8;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Structure-specific endonuclease subunit SLX4 {ECO:0000255|HAMAP-Rule:MF_03110};
GN   Name=SLX4 {ECO:0000255|HAMAP-Rule:MF_03110}; ORFNames=PICST_28599;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Regulatory subunit of the SLX1-SLX4 structure-specific
CC       endonuclease that resolves DNA secondary structures generated during
CC       DNA repair and recombination. Has endonuclease activity towards
CC       branched DNA substrates, introducing single-strand cuts in duplex DNA
CC       close to junctions with ss-DNA. {ECO:0000255|HAMAP-Rule:MF_03110}.
CC   -!- SUBUNIT: Forms a heterodimer with SLX1. {ECO:0000255|HAMAP-
CC       Rule:MF_03110}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03110}.
CC   -!- PTM: Phosphorylated in response to DNA damage. {ECO:0000255|HAMAP-
CC       Rule:MF_03110}.
CC   -!- SIMILARITY: Belongs to the SLX4 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03110}.
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DR   EMBL; AAVQ01000001; EAZ63521.2; -; Genomic_DNA.
DR   RefSeq; XP_001387544.2; XM_001387507.1.
DR   AlphaFoldDB; A3GGG8; -.
DR   EnsemblFungi; EAZ63521; EAZ63521; PICST_28599.
DR   GeneID; 4851367; -.
DR   KEGG; pic:PICST_28599; -.
DR   eggNOG; ENOG502RS18; Eukaryota.
DR   HOGENOM; CLU_429039_0_0_1; -.
DR   InParanoid; A3GGG8; -.
DR   OMA; CKEPAND; -.
DR   OrthoDB; 1409030at2759; -.
DR   Proteomes; UP000002258; Chromosome 1.
DR   GO; GO:0033557; C:Slx1-Slx4 complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0017108; F:5'-flap endonuclease activity; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   GO; GO:0006996; P:organelle organization; IEA:UniProt.
DR   HAMAP; MF_03110; Endonuc_su_Slx4; 1.
DR   InterPro; IPR027784; Slx4_ascomycetes.
DR   InterPro; IPR018574; Structure-sp_endonuc_su_Slx4.
DR   Pfam; PF09494; Slx4; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; Nucleus; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..782
FT                   /note="Structure-specific endonuclease subunit SLX4"
FT                   /id="PRO_0000388043"
FT   REGION          63..91
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          359..425
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        367..388
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        408..425
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   782 AA;  88506 MW;  96B2C72F3BEE6A90 CRC64;
     MVDSESQNDF ANDGDNDSYF VSTQFESKQE EIMEKELQKL QTGQTVSQLL RFRSGISGLT
     SVTPKPVKVS RPGLRKTGSR KSKKNQSMSA MVKERFKTDK YAYFSGDQRK IDEFLRRLEG
     SEDIENMAMS TSGKDGSCLF TRDEWICIVQ SIKLRFPELS TTKKKSLSAI TRQINKQEKE
     NEDENSIWSQ ARSLPSLELT DEDLKWLYDL DDEQMANRTI TSSMTEVDGN DDHSPFVMTL
     SQTTPSQLSH IKESDSLYSQ ETNVQTTEPA DHQSGHMQRC HSQTAEGKTQ SKILEIEIVS
     DSEEEIESLI RNTEPDSSED VYGANEVSSH QVPAVDALAS FQSFPFAADM IPRNNVRDKE
     HESLHISSSI RSSPAQSLTQ SQVPSSIDSI IEPPCESPRK MSPIRDTQPE PVITSPFKTP
     TKKSKELLSK YSSPVKNSIH NNMGSPVAMM VPRSSESAKH VEEIIVSSDE ESVYSTAKSV
     FPSAQIVISE VEDEDEEFYE IVSSIPEKHK PTAAAKKRKL LQTSRYEVVS NFNINDYDDD
     QRGFKLRKLE TKPIIIDSDN EIADSEEDEK NLSIIEITRE VEAEESEHDT EYLINLGKQV
     EGNSKMNTSV LQVPSSPSSI TFGRTDILKE LEAFSNSDLD TDRTINSNTS KNSNAIKSGT
     NQTIDFTLLS TKELQERFKK WELKPVQGRQ RMVSVLSEVS KLFTNSFNDP VPETRQGFEG
     TVYGSLNRLV GSNQYWHEKI ISFEPLRVSE LRDWICTKGY ELEEDFLMRY CDDNGYCCTR
     QP