SLX9_YEAST
ID SLX9_YEAST Reviewed; 210 AA.
AC P53251;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Ribosome biogenesis protein SLX9;
GN Name=SLX9; OrderedLocusNames=YGR081C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION.
RX PubMed=14566339; DOI=10.1038/ng1258;
RA Ooi S.L., Shoemaker D.D., Boeke J.D.;
RT "DNA helicase gene interaction network defined using synthetic lethality
RT analyzed by microarray.";
RL Nat. Genet. 35:277-286(2003).
RN [6]
RP INDUCTION.
RX PubMed=16943074; DOI=10.1016/j.fm.2006.02.003;
RA Tanaka F., Ando A., Nakamura T., Takagi H., Shima J.;
RT "Functional genomic analysis of commercial baker's yeast during initial
RT stages of model dough-fermentation.";
RL Food Microbiol. 23:717-728(2006).
RN [7]
RP FUNCTION, AND INTERACTION WITH 35S PRE-RRNA; 23S PRE-RRNA; 20S PRE-RRNA AND
RP U3 SNORNA.
RX PubMed=17018574; DOI=10.1261/rna.159406;
RA Bax R., Raue H.A., Vos J.C.;
RT "Slx9p facilitates efficient ITS1 processing of pre-rRNA in Saccharomyces
RT cerevisiae.";
RL RNA 12:2005-2013(2006).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Involved in ribosome biogenesis. Required for normal pre-rRNA
CC processing in internal transcribed spacer 1 (ITS1). May be involved in
CC the movements of the replication forks. {ECO:0000269|PubMed:14566339,
CC ECO:0000269|PubMed:17018574}.
CC -!- SUBUNIT: Interacts with the 35S, 23S and 20S pre-rRNAs and with the U3
CC snoRNA. {ECO:0000269|PubMed:17018574}.
CC -!- INTERACTION:
CC P53251; P38333: ENP1; NbExp=3; IntAct=EBI-23221, EBI-6482;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:14562095}.
CC -!- INDUCTION: Transiently up-regulated during the initial 15 minutes of
CC dough-fermentation. {ECO:0000269|PubMed:16943074}.
CC -!- MISCELLANEOUS: Present with 3670 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SLX9 family. {ECO:0000305}.
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DR EMBL; Z72866; CAA97083.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08174.1; -; Genomic_DNA.
DR PIR; S64376; S64376.
DR RefSeq; NP_011595.3; NM_001181210.3.
DR AlphaFoldDB; P53251; -.
DR BioGRID; 33323; 426.
DR ComplexPortal; CPX-1604; Small ribosomal subunit processome, variant 1.
DR ComplexPortal; CPX-1607; Small ribosomal subunit processome, variant 2.
DR ComplexPortal; CPX-1608; Small ribosomal subunit processome, variant 3.
DR DIP; DIP-4723N; -.
DR IntAct; P53251; 37.
DR MINT; P53251; -.
DR STRING; 4932.YGR081C; -.
DR CarbonylDB; P53251; -.
DR iPTMnet; P53251; -.
DR MaxQB; P53251; -.
DR PaxDb; P53251; -.
DR PRIDE; P53251; -.
DR EnsemblFungi; YGR081C_mRNA; YGR081C; YGR081C.
DR GeneID; 852972; -.
DR KEGG; sce:YGR081C; -.
DR SGD; S000003313; SLX9.
DR VEuPathDB; FungiDB:YGR081C; -.
DR eggNOG; ENOG502S2IS; Eukaryota.
DR HOGENOM; CLU_1372424_0_0_1; -.
DR InParanoid; P53251; -.
DR OMA; LKPKMQD; -.
DR BioCyc; YEAST:G3O-30793-MON; -.
DR PRO; PR:P53251; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53251; protein.
DR GO; GO:0030686; C:90S preribosome; IDA:SGD.
DR GO; GO:0005730; C:nucleolus; IDA:ComplexPortal.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0030688; C:preribosome, small subunit precursor; IDA:SGD.
DR GO; GO:0032040; C:small-subunit processome; IPI:ComplexPortal.
DR GO; GO:0051880; F:G-quadruplex DNA binding; IDA:SGD.
DR GO; GO:0030490; P:maturation of SSU-rRNA; IC:ComplexPortal.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0000056; P:ribosomal small subunit export from nucleus; IMP:SGD.
DR InterPro; IPR028160; Slx9-like.
DR Pfam; PF15341; SLX9; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Ribosome biogenesis; rRNA processing.
FT CHAIN 1..210
FT /note="Ribosome biogenesis protein SLX9"
FT /id="PRO_0000202808"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 210 AA; 24069 MW; 375042316F8A5C37 CRC64;
MVAKKRNTLR SKVSARNSQN FGPDVANNGI LDESYDIESD PRAFLHQPKE TKKEKLLNRQ
NTFLSNLKGK STLNDGIAAN FDGISKSSIR RRKRKLREEL KPRMQDLLTS LEQEKDLRGI
IENSSKDMNN DDDIDMDSKI RFVDTKEMNL KKIEPGSVRI KKNQPNIRNQ KGAKALAANE
TARFNQVLTN QDFQKNPFGA LREVIKLQKQ
