ID   SLYA_PECCC              Reviewed;         145 AA.
AC   Q9RB09;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Transcriptional regulator SlyA {ECO:0000255|HAMAP-Rule:MF_01819};
DE   AltName: Full=Homolog of Rap {ECO:0000303|PubMed:9402023};
DE            Short=Hor-Er {ECO:0000303|PubMed:9402023};
GN   Name=slyA {ECO:0000255|HAMAP-Rule:MF_01819};
GN   Synonyms=hor {ECO:0000303|PubMed:9402023};
OS   Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp.
OS   carotovora).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=555;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 39048 / GS101 / SC 12;
RX   PubMed=9402023; DOI=10.1046/j.1365-2958.1997.5981976.x;
RA   Thomson N.R., Cox A., Bycroft B.W., Stewart G.S.A.B., Williams P.,
RA   Salmond G.P.C.;
RT   "The rap and hor proteins of Erwinia, Serratia and Yersinia: a novel
RT   subgroup in a growing superfamily of proteins regulating diverse
RT   physiological processes in bacterial pathogens.";
RL   Mol. Microbiol. 26:531-544(1997).
RN   [2]
RP   FUNCTION.
RX   PubMed=12612613; DOI=10.1038/sj.embor.embor730;
RA   Basset A., Tzou P., Lemaitre B., Boccard F.;
RT   "A single gene that promotes interaction of a phytopathogenic bacterium
RT   with its insect vector, Drosophila melanogaster.";
RL   EMBO Rep. 4:205-209(2003).
CC   -!- FUNCTION: Transcription regulator that can specifically activate or
CC       repress expression of target genes (By similarity). Regulates genes
CC       involved in production of antibiotic and exoenzyme virulence
CC       determinants in the phytopathogen (PubMed:9402023). Required for the
CC       expression of the virulence protein evf during Drosophila melanogaster
CC       infection (PubMed:12612613). {ECO:0000255|HAMAP-Rule:MF_01819,
CC       ECO:0000269|PubMed:12612613, ECO:0000305|PubMed:9402023}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01819}.
CC   -!- DISRUPTION PHENOTYPE: Pleiotropic, with decreased production of
CC       exoenzymes such as pectate lyase (Pel), cellulase and protease Prt. Has
CC       a 50% decrease in potato macerationafter 96 hours. Dramatically reduces
CC       transcription of carA, the antibiotic carbapenem biosynthetic gene.
CC       {ECO:0000269|PubMed:9402023}.
CC   -!- SIMILARITY: Belongs to the SlyA family. {ECO:0000255|HAMAP-
CC       Rule:MF_01819}.
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DR   EMBL; AF168687; AAD50820.1; -; Genomic_DNA.
DR   RefSeq; WP_010276609.1; NZ_VBUA01000006.1.
DR   AlphaFoldDB; Q9RB09; -.
DR   SMR; Q9RB09; -.
DR   GeneID; 51389356; -.
DR   GeneID; 61408321; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   HAMAP; MF_01819; HTH_type_SlyA; 1.
DR   InterPro; IPR000835; HTH_MarR-typ.
DR   InterPro; IPR023187; Tscrpt_reg_MarR-type_CS.
DR   InterPro; IPR023071; Tscrpt_reg_SlyA.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01047; MarR; 1.
DR   PRINTS; PR00598; HTHMARR.
DR   SMART; SM00347; HTH_MARR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS01117; HTH_MARR_1; 1.
DR   PROSITE; PS50995; HTH_MARR_2; 1.
PE   3: Inferred from homology;
KW   Activator; Antibiotic biosynthesis; DNA-binding; Repressor; Transcription;
KW   Transcription regulation; Virulence.
FT   CHAIN           1..145
FT                   /note="Transcriptional regulator SlyA"
FT                   /id="PRO_0000054388"
FT   DOMAIN          2..135
FT                   /note="HTH marR-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01819"
FT   DNA_BIND        49..72
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01819"
SQ   SEQUENCE   145 AA;  16417 MW;  7F9FC1794C5AF5DC CRC64;
     MELPLGSDLA RLVRVWRALV DHRLKPLELT QTHWVTLHNI YHLPPGQSQI QLAKAIGIEQ
     PSLVRTLDQL EEKGLITRHV CAHDRRAKRI MLTESAEPII QAVNGVISHT RSEVLFGITP
     EQVDELALLV SRLEKNILAL HENQA