ID   SLYA_SERS3              Reviewed;         145 AA.
AC   Q9RA96; A0A2I5TJT9;
DT   24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Transcriptional regulator SlyA {ECO:0000255|HAMAP-Rule:MF_01819};
DE   AltName: Full=Regulator of antibiotic and pigment protein {ECO:0000303|PubMed:9402023};
DE            Short=Rap {ECO:0000303|PubMed:9402023};
GN   Name=slyA {ECO:0000255|HAMAP-Rule:MF_01819};
GN   Synonyms=rap {ECO:0000303|PubMed:9402023}; ORFNames=Ser39006_012270;
OS   Serratia sp. (strain ATCC 39006).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Serratia; unclassified Serratia.
OX   NCBI_TaxID=104623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 39006 / SC 11482;
RX   PubMed=9402023; DOI=10.1046/j.1365-2958.1997.5981976.x;
RA   Thomson N.R., Cox A., Bycroft B.W., Stewart G.S.A.B., Williams P.,
RA   Salmond G.P.C.;
RT   "The rap and hor proteins of Erwinia, Serratia and Yersinia: a novel
RT   subgroup in a growing superfamily of proteins regulating diverse
RT   physiological processes in bacterial pathogens.";
RL   Mol. Microbiol. 26:531-544(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39006 / SC 11482;
RX   PubMed=24336377; DOI=10.1128/genomea.01039-13;
RA   Fineran P.C., Iglesias Cans M.C., Ramsay J.P., Wilf N.M., Cossyleon D.,
RA   McNeil M.B., Williamson N.R., Monson R.E., Becher S.A., Stanton J.A.,
RA   Brugger K., Brown S.D., Salmond G.P.;
RT   "Draft genome sequence of Serratia sp. strain ATCC 39006, a model bacterium
RT   for analysis of the biosynthesis and regulation of prodigiosin, a
RT   carbapenem, and gas vesicles.";
RL   Genome Announc. 1:E01039-E01039(2013).
RN   [3]
RP   FUNCTION.
RC   STRAIN=ATCC 39006 / SC 11482;
RX   PubMed=12519208; DOI=10.1046/j.1365-2958.2003.03295.x;
RA   Slater H., Crow M., Everson L., Salmond G.P.C.;
RT   "Phosphate availability regulates biosynthesis of two antibiotics,
RT   prodigiosin and carbapenem, in Serratia via both quorum-sensing-dependent
RT   and -independent pathways.";
RL   Mol. Microbiol. 47:303-320(2003).
CC   -!- FUNCTION: Transcription regulator that can specifically activate or
CC       repress expression of target genes (By similarity). Participates in
CC       transcriptional activation of pig (prodigiosin pigment) and car
CC       (carbapenem antibiotic) gene clusters (PubMed:9402023,
CC       PubMed:12519208). {ECO:0000255|HAMAP-Rule:MF_01819,
CC       ECO:0000269|PubMed:12519208, ECO:0000305|PubMed:9402023}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01819}.
CC   -!- DISRUPTION PHENOTYPE: Loss of prodigiosin and carbapenem production.
CC       {ECO:0000269|PubMed:9402023}.
CC   -!- SIMILARITY: Belongs to the SlyA family. {ECO:0000255|HAMAP-
CC       Rule:MF_01819}.
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DR   EMBL; AF168597; AAF01153.1; -; Genomic_DNA.
DR   EMBL; CP025084; AUH04835.1; -; Genomic_DNA.
DR   RefSeq; WP_021016279.1; NZ_CP025085.1.
DR   AlphaFoldDB; Q9RA96; -.
DR   SMR; Q9RA96; -.
DR   STRING; 104623.Ser39006_03016; -.
DR   EnsemblBacteria; AUH04835; AUH04835; Ser39006_012270.
DR   KEGG; sera:Ser39006_012270; -.
DR   OrthoDB; 1744037at2; -.
DR   Proteomes; UP000017700; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   HAMAP; MF_01819; HTH_type_SlyA; 1.
DR   InterPro; IPR000835; HTH_MarR-typ.
DR   InterPro; IPR023187; Tscrpt_reg_MarR-type_CS.
DR   InterPro; IPR023071; Tscrpt_reg_SlyA.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01047; MarR; 1.
DR   PRINTS; PR00598; HTHMARR.
DR   SMART; SM00347; HTH_MARR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS01117; HTH_MARR_1; 1.
DR   PROSITE; PS50995; HTH_MARR_2; 1.
PE   3: Inferred from homology;
KW   Activator; Antibiotic biosynthesis; DNA-binding; Reference proteome;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..145
FT                   /note="Transcriptional regulator SlyA"
FT                   /id="PRO_0000054394"
FT   DOMAIN          2..135
FT                   /note="HTH marR-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01819"
FT   DNA_BIND        49..72
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01819"
SQ   SEQUENCE   145 AA;  16508 MW;  D665853A20BA6F91 CRC64;
     MELPLGSDLA RLVRVWRALI DHRLKPLELT QTHWVTLHNI HELPPGQSQI QLAKAIGIEQ
     PSLVRTLDQL EDKGLITRHI CVHDRRAKRI MLTDMADPII QAVNDVIDQT RSEILNGITP
     EEVSELATII SRLESNILSL YEMQS