BIG1_ARATH
ID BIG1_ARATH Reviewed; 1687 AA.
AC F4JSZ5; Q0WUF1; Q9SZM0;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=Brefeldin A-inhibited guanine nucleotide-exchange protein 1;
DE Short=BIG1;
DE AltName: Full=ARF guanine-nucleotide exchange factor BIG1;
GN Name=BIG1; OrderedLocusNames=At4g38200; ORFNames=F20D10.320;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 897-1687.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=12553910; DOI=10.1016/s0092-8674(03)00003-5;
RA Geldner N., Anders N., Wolters H., Keicher J., Kornberger W., Muller P.,
RA Delbarre A., Ueda T., Nakano A., Juergens G.;
RT "The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin
RT transport, and auxin-dependent plant growth.";
RL Cell 112:219-230(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14742722; DOI=10.1091/mbc.e03-06-0443;
RA Cox R., Mason-Gamer R.J., Jackson C.L., Segev N.;
RT "Phylogenetic analysis of Sec7-domain-containing Arf nucleotide
RT exchangers.";
RL Mol. Biol. Cell 15:1487-1505(2004).
RN [6]
RP GENE FAMILY.
RX PubMed=17653190; DOI=10.1038/nature05967;
RA Richter S., Geldner N., Schrader J., Wolters H., Stierhof Y.D., Rios G.,
RA Koncz C., Robinson D.G., Juergens G.;
RT "Functional diversification of closely related ARF-GEFs in protein
RT secretion and recycling.";
RL Nature 448:488-492(2007).
CC -!- FUNCTION: Activates the ARF proteins by exchanging bound GDP for free
CC GTP. Plays a role in vesicular protein sorting (By similarity).
CC {ECO:0000250}.
CC -!- ACTIVITY REGULATION: Inhibited by brefeldin A. {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}. Membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Note=Soluble and partially membrane-bound.
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB37560.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80485.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL035538; CAB37560.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161593; CAB80485.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86892.1; -; Genomic_DNA.
DR EMBL; AK227209; BAE99247.1; -; mRNA.
DR PIR; T05647; T05647.
DR RefSeq; NP_195533.2; NM_119981.4.
DR AlphaFoldDB; F4JSZ5; -.
DR SMR; F4JSZ5; -.
DR STRING; 3702.AT4G38200.1; -.
DR PaxDb; F4JSZ5; -.
DR PRIDE; F4JSZ5; -.
DR ProteomicsDB; 240344; -.
DR EnsemblPlants; AT4G38200.1; AT4G38200.1; AT4G38200.
DR GeneID; 829976; -.
DR Gramene; AT4G38200.1; AT4G38200.1; AT4G38200.
DR KEGG; ath:AT4G38200; -.
DR Araport; AT4G38200; -.
DR TAIR; locus:2120923; AT4G38200.
DR eggNOG; KOG0929; Eukaryota.
DR HOGENOM; CLU_000691_0_0_1; -.
DR InParanoid; F4JSZ5; -.
DR OMA; DLYITFF; -.
DR OrthoDB; 815698at2759; -.
DR PRO; PR:F4JSZ5; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; F4JSZ5; baseline and differential.
DR Genevisible; F4JSZ5; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0032012; P:regulation of ARF protein signal transduction; IEA:InterPro.
DR CDD; cd00171; Sec7; 1.
DR Gene3D; 1.10.1000.11; -; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR032629; DCB_dom.
DR InterPro; IPR015403; Sec7_C.
DR InterPro; IPR023394; Sec7_C_sf.
DR InterPro; IPR000904; Sec7_dom.
DR InterPro; IPR035999; Sec7_dom_sf.
DR InterPro; IPR032691; Sec7_N.
DR Pfam; PF16213; DCB; 1.
DR Pfam; PF09324; DUF1981; 1.
DR Pfam; PF01369; Sec7; 1.
DR Pfam; PF12783; Sec7_N; 1.
DR SMART; SM00222; Sec7; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF48425; SSF48425; 1.
DR PROSITE; PS50190; SEC7; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Guanine-nucleotide releasing factor; Membrane;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..1687
FT /note="Brefeldin A-inhibited guanine nucleotide-exchange
FT protein 1"
FT /id="PRO_0000420950"
FT DOMAIN 532..719
FT /note="SEC7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00189"
FT REGION 494..529
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1229..1248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 494..509
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 510..524
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 634
FT /evidence="ECO:0000255"
SQ SEQUENCE 1687 AA; 187537 MW; 3A4181CB4EE0EF69 CRC64;
MSSSQNLGGA TRCGRVIGPS LDKIIKNAAW RKHTFLVSAC KSVLDKLEAL SDSPDPSSPL
FGLTTSDADA VLQPLLLSLD TGYAKVIEPA LDCSFKLFSL SLLRGEVCSS SPDSLLYKLI
HAICKVCGIG EESIELAVLR VLLAAVRSPR ILIRGDCLLH LVRTCYNVYL GGFNGTNQIC
AKSVLAQIML IVFTRSEANS MDASLKTVNV NDLLAITDKN VNEGNSVHIC QGFINDVITA
GEAAPPPDFA LVQPPEEGAS STEDEGTGSK IREDGFLLFK NLCKLSMKFS SQENTDDQIL
VRGKTLSLEL LKVIIDNGGP IWLSDERFLN AIKQLLCLSL LKNSALSVMS IFQLQCAIFT
TLLRKYRSGM KSEVGIFFPM LVLRVLENVL QPSFVQKMTV LSLLENICHD PNLIIDIFVN
FDCDVESPNI FERIVNGLLK TALGPPPGSS TILSPVQDIT FRHESVKCLV SIIKAMGTWM
DQQLSVGDSL LPKSLENEAP ANNHSNSNEE DGTTIDHDFH PDLNPESSDA ATLEQRRAYK
IERQKGVTLF NRKPSKGIEF LISSKKVGNS PDEVVSFLRN TTGLNATMIG DYLGEREDFP
MKVMHAYVDS FDFKEMNFGE AIRFFLRGFR LPGEAQKIDR IMEKFAERFC KCNPNSFSSA
DTAYVLAYSV IMLNTDAHNI MVKEKMTKAD FIRNNRGIDD GKDLPEEYLG ALYDQVVINE
IKMSSDSSAP ESRQSNGLNK LLGLDGILNL VYWTQTEEKA VGANGLLIKD IQEKFRSKSG
KSESAYHVVT DVAILRFMVE VSWGPMLAAF SVTLDQSDDR LAAVECLRGF RYAVHVTAVM
GMQTQRDAFV TSMAKFTNLH CAGDMKQKNV DAVKAIISIA IEDGNHLQDA WEHILTCLSR
IEHLQLLGEG APSDASYFAS TETEEKKALG FPNLKKKGAL QNPVMMAVVR GGSYDSSTIG
PNMPGLVKQD QINNFIANLN LLDQIGSFQL NNVYAHSQRL KTEAIVAFVK ALCKVSMSEL
QSPTDPRVFS LTKLVEIAHY NMNRIRLVWS RIWSILSDFF VSVGLSENLS VAIFVMDSLR
QLSMKFLERE ELANYNFQNE FLRPFVIVMQ KSSSAEIREL IVRCISQMVL SRVSNVKSGW
KSVFKVFTTA AADERKNIVL LAFETMEKIV REYFSYITET EATTFTDCVR CLITFTNSTF
TSDVSLNAIA FLRFCALKLA DGGLVWNEKG RSSSPSTPVT DDHSPSTQNF MDADENISYW
VPLLTGLSKL TSDSRSAIRK SSLEVLFNIL KDHGHIFSRT FWIGVFSSVI YPIFNSVWGE
NDLLSKDEHS SFPSTFSSHP SEVSWDAETS AMAAQYLVDL FVSFFTVIRS QLSSVVSLLA
GLIRSPAQGP TVAGVGALLR LADELGDRFS ENEWKEIFLA VNEAASLTLS SFMKTLRTMD
DIPDEDTLSD QDFSNEDDID EDSLQTMSYV VARTKSHITV QLQVVQVVTD LYRIHQQSLL
ASHVTVILEI LSSISSHAHQ LNSDLILQKK VRRACSILEL SEPPMLHFEN DTFQNYLDIL
QAIVTNNPGV SLELNVESQL MTVCMQILKM YLKCTLFQGD ELEETRQPKN WILPMGAASK
EEAAARSPLV VAVLKALREL KRDSFKRYAP NFFPLLVELV RSEHSSSQVP QVLSTVFHTC
MGAMMDE
