ID   BIG1_ASHGO              Reviewed;         344 AA.
AC   Q75C24;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Protein BIG1;
DE   Flags: Precursor;
GN   Name=BIG1; OrderedLocusNames=ACR093C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 124.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Required for normal beta-1,6-glucan synthesis. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BIG1 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE016816; AAS51319.2; -; Genomic_DNA.
DR   RefSeq; NP_983495.2; NM_208848.2.
DR   AlphaFoldDB; Q75C24; -.
DR   STRING; 33169.AAS51319; -.
DR   EnsemblFungi; AAS51319; AAS51319; AGOS_ACR093C.
DR   GeneID; 4619622; -.
DR   KEGG; ago:AGOS_ACR093C; -.
DR   eggNOG; ENOG502RXHV; Eukaryota.
DR   HOGENOM; CLU_067894_0_0_1; -.
DR   InParanoid; Q75C24; -.
DR   OMA; PNWNPIR; -.
DR   Proteomes; UP000000591; Chromosome III.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0006078; P:(1->6)-beta-D-glucan biosynthetic process; IBA:GO_Central.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009272; P:fungal-type cell wall biogenesis; IBA:GO_Central.
DR   InterPro; IPR037654; Big1.
DR   PANTHER; PTHR28285; PTHR28285; 1.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Endoplasmic reticulum; Glycoprotein;
KW   Membrane; Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..344
FT                   /note="Protein BIG1"
FT                   /id="PRO_0000277848"
FT   TOPO_DOM        19..275
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        276..296
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        297..344
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        47
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   344 AA;  39105 MW;  70DD39211B514612 CRC64;
     MQNVASWLLA LVVAVVQGEL LPGKSAPALL WSYKLSEGIQ EYQLAYNMTT VLPAPEFNAI
     ALELLDHCNS HAYVFVNQPG LRLEDFDYED AWTVLPNYLS RSSSALRFEQ VEVSPSNVFE
     NLIAHTKRRC DVQREIILRA EQTNQFEPYI DAQSRIIQVH FSPLPGDGRN ERPSREDVLA
     DHDQRLRRIL GRLPSPAVTV IYTSLEPADR LSASPPRAGI FPEIFEHESR RIEYERNDRD
     LQVNRYFPSH HPKMEPIEEV ELSLLDPKFI QSNLKLLKLI AVSAIGSLTW QLYSLFSPKL
     PVATPKGTAK RQKGQKKLVK LATAQAAKQA EEVKLEVSQQ EKED