ID   BIG1_DEBHA              Reviewed;         321 AA.
AC   Q6BHZ8;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 2.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Protein BIG1;
DE   Flags: Precursor;
GN   Name=BIG1; OrderedLocusNames=DEHA2G14542g;
OS   Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990
OS   / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Debaryomyces.
OX   NCBI_TaxID=284592;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for normal beta-1,6-glucan synthesis. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BIG1 family. {ECO:0000305}.
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DR   EMBL; CR382139; CAG90661.2; -; Genomic_DNA.
DR   RefSeq; XP_462173.2; XM_462173.1.
DR   AlphaFoldDB; Q6BHZ8; -.
DR   STRING; 4959.XP_462173.2; -.
DR   EnsemblFungi; CAG90661; CAG90661; DEHA2G14542g.
DR   GeneID; 2905090; -.
DR   KEGG; dha:DEHA2G14542g; -.
DR   VEuPathDB; FungiDB:DEHA2G14542g; -.
DR   eggNOG; ENOG502RXHV; Eukaryota.
DR   HOGENOM; CLU_067894_0_0_1; -.
DR   InParanoid; Q6BHZ8; -.
DR   OMA; PNWNPIR; -.
DR   OrthoDB; 1522306at2759; -.
DR   Proteomes; UP000000599; Chromosome G.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   InterPro; IPR037654; Big1.
DR   PANTHER; PTHR28285; PTHR28285; 1.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Endoplasmic reticulum; Glycoprotein;
KW   Membrane; Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..321
FT                   /note="Protein BIG1"
FT                   /id="PRO_0000277851"
FT   TOPO_DOM        24..279
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        280..300
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        301..321
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        42
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   321 AA;  37170 MW;  4ABAE3B7CCFEC42F CRC64;
     MFISIRRLVA LVAITQLVAS FSAVPVLVAS HRLAKGLKEE LNGSGPQAPE SATNLIKRLV
     TECSSDEYLV INQPGLVLED MTVNEADNWP FLRRYIAMAS SVVGIPWVRG ALDLDFIEQY
     IISTCHAETM NVVHDDDQEV GNYIDTRTRV IKIDLSELPP RSDLELRYSV IREHDELIRK
     IIRKLPSPHY TIILTSDMPQ NVHPIPNMVV ESQPDKYQIF HDLVNDPSRE LEIERNDRFH
     QTIEPYWSPD RNTINRYMEN KKKDEIHFFD YDLWTRNEKL ILTIIVMVLT LFTFQLTKLV
     KLLTETVFKK DKGLITHTKS E