ID   BIG1_KLULA              Reviewed;         323 AA.
AC   Q6CMA4; B4UN79;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Protein BIG1;
DE   Flags: Precursor;
GN   Name=BIG1; OrderedLocusNames=KLLA0E21737g, KLLA0E21835g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for normal beta-1,6-glucan synthesis. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BIG1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAR56750.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; CR382125; CAR56750.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_002999412.1; XM_002999366.1.
DR   AlphaFoldDB; Q6CMA4; -.
DR   STRING; 28985.XP_002999412.1; -.
DR   PRIDE; Q6CMA4; -.
DR   EnsemblFungi; CAR56750; CAR56750; KLLA0_E21737g.
DR   GeneID; 9487375; -.
DR   KEGG; kla:KLLA0_E21737g; -.
DR   eggNOG; ENOG502RXHV; Eukaryota.
DR   HOGENOM; CLU_067894_0_0_1; -.
DR   InParanoid; Q6CMA4; -.
DR   OMA; PNWNPIR; -.
DR   Proteomes; UP000000598; Chromosome E.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IEA:EnsemblFungi.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009272; P:fungal-type cell wall biogenesis; IEA:EnsemblFungi.
DR   InterPro; IPR037654; Big1.
DR   PANTHER; PTHR28285; PTHR28285; 1.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Endoplasmic reticulum; Glycoprotein;
KW   Membrane; Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..323
FT                   /note="Protein BIG1"
FT                   /id="PRO_0000277852"
FT   TOPO_DOM        24..281
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        282..302
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        303..323
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        145
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   323 AA;  37243 MW;  21B967A1AD98B2CF CRC64;
     MQPSFMTMNE LIVFALLLTP LLAARQQSVP GLLFSYNLAP GIVKYQDKYD NAQLPQDDFL
     KVAKDLISQC HSETYVFVNQ PGLSRSDFKQ HKQSMHKLYN YVLTSSTTVK FEKIDIVQND
     DIYEELIAYT MDKCNIDDKI DIGGNVTDRY QPFVEARTRV LRVDFPPLPQ QGQYVEGIGT
     RKDVLQANDE YLRYVMGTLP TPKHTVFYMS LEKSEVPDTE TSLSYDIWPE IFTNPQRKVE
     IDRNDRVAKE MPKLVPYRPR IDTEDDKYLT VLDQEFIDNH YGIIVLIIGV TIAFILLQLG
     AVKVKKPSKP QITKDVTKEK KTQ