ID   SM50_STRPU              Reviewed;         445 AA.
AC   P11994;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=50 kDa spicule matrix protein;
DE   Flags: Precursor;
GN   Name=SM50;
OS   Strongylocentrotus purpuratus (Purple sea urchin).
OC   Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC   Euechinoidea; Echinacea; Camarodonta; Echinidea; Strongylocentrotidae;
OC   Strongylocentrotus.
OX   NCBI_TaxID=7668;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3030858; DOI=10.1016/0012-1606(87)90254-5;
RA   Sucov H.M., Benson S.C., Robinson J.J., Britten R.J., Wilt F.H.,
RA   Davidson E.H.;
RT   "A lineage-specific gene encoding a major matrix protein of the sea urchin
RT   embryo spicule. II. Structure of the gene and derived sequence of the
RT   protein.";
RL   Dev. Biol. 120:507-519(1987).
RN   [2]
RP   SEQUENCE REVISION.
RX   PubMed=2019323; DOI=10.1016/0012-1606(91)90226-s;
RA   Katoh-Fukui Y., Noce T., Ueda T., Fujiwara Y., Hashimoto N.,
RA   Higashinakagawa T., Killian C.E., Livingston B.T., Wilt F.H., Benson S.C.,
RA   Sucov H.M., Davidson E.H.;
RT   "The corrected structure of the SM50 spicule matrix protein of
RT   Strongylocentrotus purpuratus.";
RL   Dev. Biol. 145:201-202(1991).
CC   -!- FUNCTION: Major matrix protein of the sea urchin embryo spicule. The
CC       function of the matrix proteins is to direct crystal growth in certain
CC       orientations and inhibit growth in others.
CC   -!- TISSUE SPECIFICITY: Expressed specifically in the micromere/primary
CC       mesenchyme cells (PMC) lineage.
CC   -!- DEVELOPMENTAL STAGE: Detected at hatching blastula, it remains at a
CC       certain level from the mesenchyme blastula to the pluteus stages.
CC   -!- DOMAIN: The repetitive domain may provide a calcite binding matrix.
CC   -!- SIMILARITY: Belongs to the SM50 family. {ECO:0000305}.
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DR   EMBL; M16231; AAA30071.1; -; Genomic_DNA.
DR   EMBL; M16230; AAA30071.1; JOINED; Genomic_DNA.
DR   PIR; A27263; A27263.
DR   AlphaFoldDB; P11994; -.
DR   STRING; 7668.SPU_018811-tr; -.
DR   PRIDE; P11994; -.
DR   eggNOG; KOG3544; Eukaryota.
DR   HOGENOM; CLU_674973_0_0_1; -.
DR   Proteomes; UP000007110; Unassembled WGS sequence.
DR   Gene3D; 3.10.100.10; -; 1.
DR   InterPro; IPR001304; C-type_lectin-like.
DR   InterPro; IPR016186; C-type_lectin-like/link_sf.
DR   InterPro; IPR016187; CTDL_fold.
DR   InterPro; IPR009765; Pericardin-like_rpt.
DR   Pfam; PF07054; Pericardin_rpt; 6.
DR   SMART; SM00034; CLECT; 1.
DR   SUPFAM; SSF56436; SSF56436; 1.
DR   PROSITE; PS50041; C_TYPE_LECTIN_2; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; Repeat; Signal.
FT   SIGNAL          1..15
FT   CHAIN           16..445
FT                   /note="50 kDa spicule matrix protein"
FT                   /id="PRO_0000017561"
FT   DOMAIN          29..159
FT                   /note="C-type lectin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT   REGION          118..178
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          210..445
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        127..150
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        304..324
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        415..429
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   445 AA;  46262 MW;  10D08FDE088E639A CRC64;
     MKGVLFIVAS LIAFATGQDC PAYYVRSQSG QSCYRYFNMR VPYRMASEFC EMVTPCGNGP
     AKMGALASVS SPQENMEIYQ LVAGFSQDNQ MENEVWLGWN SQSPFFWEDG TPAYPNGFAA
     FSSSPASPPR PGMPPTRSWP VNPQNPMSGP PGRAPVMKRQ NPPVRPGQGG RQIPQGVGPQ
     WEAVEVTAMR AFVCEVPAGR NIPIGQQPGM GQGGFGNQQP GMGGRQPGFG NQPGMGGRQP
     GFGNQPGMGG RQPGWGNQPG VGGRQPGMGG QQPGWGNQPG VGGRQPGMGG QPGVGGRQPG
     FGNQPGMVDN NQAWWTTTRL GNQPGVGGRQ PGMGGQPGVG GRQPGVGGRQ PGFGNQPGVG
     GRQPGMGGQQ PGMGGQPGVG GRQPGMGGRQ PGFGNQPGVG GRQPGMGGQQ PNNPNNPNPN
     NPNNPNNPNP RFNRPRMLQE ADALA