SMA2_YEAST
ID SMA2_YEAST Reviewed; 369 AA.
AC Q04658; D6VZA7;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Spore membrane assembly protein 2;
GN Name=SMA2; OrderedLocusNames=YML066C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP FUNCTION.
RX PubMed=11470404; DOI=10.1016/s0960-9822(01)00274-3;
RA Rabitsch K.P., Toth A., Galova M., Schleiffer A., Schaffner G., Aigner E.,
RA Rupp C., Penkner A.M., Moreno-Borchart A.C., Primig M., Esposito R.E.,
RA Klein F., Knop M., Nasmyth K.;
RT "A screen for genes required for meiosis and spore formation based on
RT whole-genome expression.";
RL Curr. Biol. 11:1001-1009(2001).
RN [5]
RP FUNCTION.
RX PubMed=12432101; DOI=10.1073/pnas.202604399;
RA Deutschbauer A.M., Williams R.M., Chu A.M., Davis R.W.;
RT "Parallel phenotypic analysis of sporulation and postgermination growth in
RT Saccharomyces cerevisiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:15530-15535(2002).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17298976; DOI=10.1242/jcs.03405;
RA Nakanishi H., Suda Y., Neiman A.M.;
RT "Erv14 family cargo receptors are necessary for ER exit during sporulation
RT in Saccharomyces cerevisiae.";
RL J. Cell Sci. 120:908-916(2007).
CC -!- FUNCTION: Involved in spore and ascus formation. Required for the
CC efficient assembly of the precursors of the prospore membrane to a
CC continuous prospore membrane. {ECO:0000269|PubMed:11470404,
CC ECO:0000269|PubMed:12432101, ECO:0000269|PubMed:17298976}.
CC -!- SUBCELLULAR LOCATION: Prospore membrane {ECO:0000269|PubMed:17298976};
CC Multi-pass membrane protein {ECO:0000269|PubMed:17298976}. Endoplasmic
CC reticulum {ECO:0000269|PubMed:17298976}. Note=Localizes to prospore
CC membrane, and accumulates in the endoplasmic reticulum in vegetative
CC and sporulating ERV14-deleted cells.
CC -!- SIMILARITY: Belongs to the SMA2 family. {ECO:0000305}.
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DR EMBL; Z38114; CAA86255.1; -; Genomic_DNA.
DR EMBL; AY557993; AAS56319.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09831.1; -; Genomic_DNA.
DR PIR; S48332; S48332.
DR RefSeq; NP_013645.1; NM_001182425.1.
DR AlphaFoldDB; Q04658; -.
DR BioGRID; 35100; 220.
DR IntAct; Q04658; 3.
DR MINT; Q04658; -.
DR STRING; 4932.YML066C; -.
DR PaxDb; Q04658; -.
DR PRIDE; Q04658; -.
DR EnsemblFungi; YML066C_mRNA; YML066C; YML066C.
DR GeneID; 854936; -.
DR KEGG; sce:YML066C; -.
DR SGD; S000004531; SMA2.
DR VEuPathDB; FungiDB:YML066C; -.
DR eggNOG; ENOG502QW7F; Eukaryota.
DR HOGENOM; CLU_776604_0_0_1; -.
DR InParanoid; Q04658; -.
DR OMA; TIDMGWS; -.
DR BioCyc; YEAST:G3O-32661-MON; -.
DR PRO; PR:Q04658; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04658; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0000329; C:fungal-type vacuole membrane; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005628; C:prospore membrane; IDA:SGD.
DR GO; GO:0032120; P:ascospore-type prospore membrane formation; IMP:SGD.
DR GO; GO:0070583; P:spore membrane bending pathway; IMP:SGD.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Membrane; Reference proteome; Sporulation;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..369
FT /note="Spore membrane assembly protein 2"
FT /id="PRO_0000203252"
FT TOPO_DOM 1..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..220
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..265
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 287..319
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 341..369
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 348..369
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 369 AA; 40872 MW; 37CA79238F4CCAEF CRC64;
MLFPKRLIVW GVLLILSLSQ FVLYLPATTC TNSKGLRLCA PQFTITVIGG SSTANEFIAS
VREFLRLISY LTIDMGWSNE FTDPSVYEDE NLVDTFQPDK VFELNYFGFC KRSNKSKVYC
TSNENYGMDV LEVLVRDVGI QLGNISTTRS NETKKFGDSL VLTYRLALTS IRDFLKHDKH
TGNALSKALI GSPDPNVKGV SPTKNYLKGV NLAFILMMFN GMVFYFAVLE IIVGFLSICV
VSAFGGALSV GKRHRLFPML LKSSSSILVV IATLTILCNI VYLIALKTLE PEEVTDVGSD
NAAVHTTGWE LLKVNVGSGF IMGLARYAIQ WVLLVLAFLA ANHYKAKPKK SDKYTEDTSN
SPSPDLMEK
