BIG1_YEAST
ID BIG1_YEAST Reviewed; 335 AA.
AC P38813; D3DL51;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Protein BIG1;
DE AltName: Full=Bad in glucose protein 1;
DE Flags: Precursor;
GN Name=BIG1; OrderedLocusNames=YHR101C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=9545237; DOI=10.1093/emboj/17.8.2235;
RA Bickle M., Delley P.-A., Schmidt A., Hall M.N.;
RT "Cell wall integrity modulates RHO1 activity via the exchange factor
RT ROM2.";
RL EMBO J. 17:2235-2245(1998).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND GLYCOSYLATION.
RX PubMed=12112232; DOI=10.1002/yea.873;
RA Azuma M., Levinson J.N., Page N., Bussey H.;
RT "Saccharomyces cerevisiae Big1p, a putative endoplasmic reticulum membrane
RT protein required for normal levels of cell wall beta-1,6-glucan.";
RL Yeast 19:783-793(2002).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for normal beta-1,6-glucan synthesis.
CC {ECO:0000269|PubMed:12112232, ECO:0000269|PubMed:9545237}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:12112232, ECO:0000269|PubMed:14562095}; Single-pass
CC type I membrane protein {ECO:0000269|PubMed:12112232,
CC ECO:0000269|PubMed:14562095}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:12112232}.
CC -!- MISCELLANEOUS: Present with 3460 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the BIG1 family. {ECO:0000305}.
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DR EMBL; U00059; AAB68861.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06795.1; -; Genomic_DNA.
DR PIR; S48965; S48965.
DR RefSeq; NP_011969.1; NM_001179231.1.
DR AlphaFoldDB; P38813; -.
DR BioGRID; 36534; 275.
DR IntAct; P38813; 1.
DR STRING; 4932.YHR101C; -.
DR iPTMnet; P38813; -.
DR MaxQB; P38813; -.
DR PaxDb; P38813; -.
DR PRIDE; P38813; -.
DR EnsemblFungi; YHR101C_mRNA; YHR101C; YHR101C.
DR GeneID; 856501; -.
DR KEGG; sce:YHR101C; -.
DR SGD; S000001143; BIG1.
DR VEuPathDB; FungiDB:YHR101C; -.
DR eggNOG; ENOG502RXHV; Eukaryota.
DR HOGENOM; CLU_067894_0_0_1; -.
DR InParanoid; P38813; -.
DR OMA; PNWNPIR; -.
DR BioCyc; YEAST:G3O-31146-MON; -.
DR PRO; PR:P38813; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38813; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IDA:SGD.
DR GO; GO:0006078; P:(1->6)-beta-D-glucan biosynthetic process; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0009272; P:fungal-type cell wall biogenesis; IMP:SGD.
DR InterPro; IPR037654; Big1.
DR PANTHER; PTHR28285; PTHR28285; 1.
PE 1: Evidence at protein level;
KW Cell wall biogenesis/degradation; Endoplasmic reticulum; Glycoprotein;
KW Membrane; Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..335
FT /note="Protein BIG1"
FT /id="PRO_0000014327"
FT TOPO_DOM 20..275
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..296
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 297..335
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 24
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 144
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 335 AA; 39069 MW; BA2D68E3A7B8884C CRC64;
MQTVLKYLLL IMCGSFCASE ELQNQTNVPA IFFSYKLTPG ILKYQEDYDR AVTLPRDTFI
EAAEKFLGVC NADTYVFINQ PGLRKLDFLE FETEFVSLQR YIRRSSTAIK FEKVDLLPQD
LYYDLAEFVK EYCNVDQVLN LRGNNTEDFQ PFIDSEKRVI IIEYPKLPED TNERKEAFRH
YDKYLRTILA QIPSPEQNVI YTSLNPGTTL AHESIIPIQI FPDIFDIKSR VGEVEQNNRV
LDVPRLSFND YTPRFSEPPS EYVSIFDSQL IENNRGLLQL IFTILVGYIL IQFFFTKKTI
VDEKITNKKD NVKQTSPQLL KKVQEIQKKP SQQVS
