SMAKA_MOUSE
ID SMAKA_MOUSE Reviewed; 106 AA.
AC Q9CPS8;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Small membrane A-kinase anchor protein;
DE Short=Small membrane AKAP;
DE Short=smAKAP;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Pancreas, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=23115245; DOI=10.1074/jbc.m112.395970;
RA Burgers P.P., Ma Y., Margarucci L., Mackey M., van der Heyden M.A.,
RA Ellisman M., Scholten A., Taylor S.S., Heck A.J.;
RT "A small novel A-kinase anchoring protein (AKAP) that localizes
RT specifically protein kinase A-regulatory subunit I (PKA-RI) to the plasma
RT membrane.";
RL J. Biol. Chem. 287:43789-43797(2012).
CC -!- FUNCTION: Binds to type I regulatory subunits of protein kinase A (PKA-
CC RI) and may anchor/target them to the plasma membrane. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with PKA type I regulatory subunits PRKAR1A and
CC PRKAR1B. Also binds to type II regulatory subunits, but at a tenfold
CC lower affinity (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Widely expressed, with very low levels in spleen
CC and liver. {ECO:0000269|PubMed:23115245}.
CC -!- PTM: May be palmitoylated at Cys-3. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the small membrane AKAP family. {ECO:0000305}.
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DR EMBL; AK006110; BAB24413.1; -; mRNA.
DR EMBL; AK006523; BAB24632.1; -; mRNA.
DR EMBL; AK010796; BAB27186.1; -; mRNA.
DR EMBL; BC048645; AAH48645.1; -; mRNA.
DR CCDS; CCDS14949.1; -.
DR RefSeq; NP_659202.1; NM_144953.2.
DR RefSeq; XP_006496261.1; XM_006496198.3.
DR RefSeq; XP_006496262.1; XM_006496199.3.
DR RefSeq; XP_006496263.1; XM_006496200.3.
DR RefSeq; XP_006496264.1; XM_006496201.3.
DR RefSeq; XP_011236868.1; XM_011238566.2.
DR AlphaFoldDB; Q9CPS8; -.
DR STRING; 10090.ENSMUSP00000055413; -.
DR iPTMnet; Q9CPS8; -.
DR PhosphoSitePlus; Q9CPS8; -.
DR MaxQB; Q9CPS8; -.
DR PaxDb; Q9CPS8; -.
DR PeptideAtlas; Q9CPS8; -.
DR PRIDE; Q9CPS8; -.
DR Antibodypedia; 62058; 44 antibodies from 11 providers.
DR DNASU; 67080; -.
DR Ensembl; ENSMUST00000050567; ENSMUSP00000055413; ENSMUSG00000043629.
DR Ensembl; ENSMUST00000114492; ENSMUSP00000110136; ENSMUSG00000043629.
DR Ensembl; ENSMUST00000114493; ENSMUSP00000110137; ENSMUSG00000043629.
DR Ensembl; ENSMUST00000186266; ENSMUSP00000140273; ENSMUSG00000043629.
DR Ensembl; ENSMUST00000190726; ENSMUSP00000140530; ENSMUSG00000043629.
DR Ensembl; ENSMUST00000190831; ENSMUSP00000140160; ENSMUSG00000043629.
DR Ensembl; ENSMUST00000191441; ENSMUSP00000139750; ENSMUSG00000043629.
DR GeneID; 67080; -.
DR KEGG; mmu:67080; -.
DR UCSC; uc007ayr.1; mouse.
DR MGI; MGI:1914330; 1700019D03Rik.
DR VEuPathDB; HostDB:ENSMUSG00000043629; -.
DR eggNOG; ENOG502S8S1; Eukaryota.
DR GeneTree; ENSGT00390000011537; -.
DR HOGENOM; CLU_187810_0_0_1; -.
DR InParanoid; Q9CPS8; -.
DR OMA; CIKSKDA; -.
DR OrthoDB; 1536209at2759; -.
DR PhylomeDB; Q9CPS8; -.
DR TreeFam; TF338355; -.
DR BioGRID-ORCS; 67080; 0 hits in 72 CRISPR screens.
DR PRO; PR:Q9CPS8; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q9CPS8; protein.
DR Bgee; ENSMUSG00000043629; Expressed in seminiferous tubule of testis and 154 other tissues.
DR Genevisible; Q9CPS8; MM.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0034237; F:protein kinase A regulatory subunit binding; IEA:InterPro.
DR InterPro; IPR027969; Small_membr_AKAP.
DR PANTHER; PTHR36471; PTHR36471; 1.
DR Pfam; PF15127; SmAKAP; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Lipoprotein; Membrane; Myristate; Palmitate; Phosphoprotein;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..106
FT /note="Small membrane A-kinase anchor protein"
FT /id="PRO_0000348431"
FT REGION 62..85
FT /note="PKA-RI-binding"
FT /evidence="ECO:0000250"
FT MOD_RES 40
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 98
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BSF0"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255"
FT LIPID 3
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 106 AA; 11926 MW; FB7560A5419DD80A CRC64;
MGCMKSKETF PFPTTLDIDK LHESEEAFIP DDSSQYRTPS PGEQQQVQEV KKLPEPGAVI
GALILEFADR LASEIVEDAL QQWACENIQY YNIPYIESEG SDTTIN
