BIG4_ARATH
ID BIG4_ARATH Reviewed; 1706 AA.
AC F4JN05; O65490;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Brefeldin A-inhibited guanine nucleotide-exchange protein 4;
DE Short=BIG4;
DE AltName: Full=ARF guanine-nucleotide exchange factor BIG4;
GN Name=BIG4; OrderedLocusNames=At4g35380; ORFNames=F23E12.60;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=12553910; DOI=10.1016/s0092-8674(03)00003-5;
RA Geldner N., Anders N., Wolters H., Keicher J., Kornberger W., Muller P.,
RA Delbarre A., Ueda T., Nakano A., Juergens G.;
RT "The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin
RT transport, and auxin-dependent plant growth.";
RL Cell 112:219-230(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14742722; DOI=10.1091/mbc.e03-06-0443;
RA Cox R., Mason-Gamer R.J., Jackson C.L., Segev N.;
RT "Phylogenetic analysis of Sec7-domain-containing Arf nucleotide
RT exchangers.";
RL Mol. Biol. Cell 15:1487-1505(2004).
RN [5]
RP GENE FAMILY.
RX PubMed=17653190; DOI=10.1038/nature05967;
RA Richter S., Geldner N., Schrader J., Wolters H., Stierhof Y.D., Rios G.,
RA Koncz C., Robinson D.G., Juergens G.;
RT "Functional diversification of closely related ARF-GEFs in protein
RT secretion and recycling.";
RL Nature 448:488-492(2007).
CC -!- FUNCTION: Activates the ARF proteins by exchanging bound GDP for free
CC GTP. Plays a role in vesicular protein sorting (By similarity).
CC {ECO:0000250}.
CC -!- ACTIVITY REGULATION: Inhibited by brefeldin A. {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}. Membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Note=Soluble and partially membrane-bound.
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA18731.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80255.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL022604; CAA18731.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161587; CAB80255.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86505.1; -; Genomic_DNA.
DR PIR; T06119; T06119.
DR RefSeq; NP_195264.2; NM_119704.4.
DR AlphaFoldDB; F4JN05; -.
DR SMR; F4JN05; -.
DR STRING; 3702.AT4G35380.1; -.
DR PaxDb; F4JN05; -.
DR PRIDE; F4JN05; -.
DR ProteomicsDB; 240628; -.
DR EnsemblPlants; AT4G35380.1; AT4G35380.1; AT4G35380.
DR GeneID; 829691; -.
DR Gramene; AT4G35380.1; AT4G35380.1; AT4G35380.
DR KEGG; ath:AT4G35380; -.
DR Araport; AT4G35380; -.
DR TAIR; locus:2122143; AT4G35380.
DR eggNOG; KOG0929; Eukaryota.
DR HOGENOM; CLU_000691_0_0_1; -.
DR InParanoid; F4JN05; -.
DR OMA; NNDDQIM; -.
DR OrthoDB; 815698at2759; -.
DR PRO; PR:F4JN05; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; F4JN05; baseline and differential.
DR Genevisible; F4JN05; AT.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR GO; GO:0032588; C:trans-Golgi network membrane; IDA:TAIR.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0032012; P:regulation of ARF protein signal transduction; IEA:InterPro.
DR CDD; cd00171; Sec7; 1.
DR Gene3D; 1.10.1000.11; -; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR032629; DCB_dom.
DR InterPro; IPR032817; Mon2_C.
DR InterPro; IPR015403; Sec7_C.
DR InterPro; IPR023394; Sec7_C_sf.
DR InterPro; IPR000904; Sec7_dom.
DR InterPro; IPR035999; Sec7_dom_sf.
DR InterPro; IPR032691; Sec7_N.
DR Pfam; PF16213; DCB; 1.
DR Pfam; PF09324; DUF1981; 1.
DR Pfam; PF16206; Mon2_C; 1.
DR Pfam; PF01369; Sec7; 1.
DR Pfam; PF12783; Sec7_N; 1.
DR SMART; SM00222; Sec7; 1.
DR SUPFAM; SSF48371; SSF48371; 2.
DR SUPFAM; SSF48425; SSF48425; 1.
DR PROSITE; PS50190; SEC7; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Guanine-nucleotide releasing factor; Membrane;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..1706
FT /note="Brefeldin A-inhibited guanine nucleotide-exchange
FT protein 4"
FT /id="PRO_0000420953"
FT DOMAIN 555..742
FT /note="SEC7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00189"
FT ACT_SITE 657
FT /evidence="ECO:0000255"
SQ SEQUENCE 1706 AA; 190826 MW; 30A40520BA63068C CRC64;
MSTSQTLGGA TRCGRIIGPS LDKIIKNAAW RKHTYLVSSC KSVLDKLESL PDDFHDPSSV
VSGLAASDAD SVLQPFLLSL ETAYSKVVEP SLDCAFKLFS LSILRGEIQS SKQDSILFKL
VNAVSKVGAI AEEPIQLAVL RVLLAAVRSP CILIRGDCLL HVVKTCYNIY LGGLSGTTQI
CAKSVLAQMM LVIFTRSEED SLDVSVKTIY VNELLTFTDK SVNEGSSVYF CQGFVNEVMA
AGQGSPLPPP DVIQILLQNP ETETVMTPDS PSFRGYVANG EGDSETGDMS KVRQDAFLLF
KNLCKLSMRF SSKENNDDQI MVRGKTLSLE LLKVIIDNGG SVWRTNESFI NAVKQYLCLS
LLKNSAVSIM SIFQLQCAIF MSLLSKLRSV LKAEIGIFFP MIVLRVLENV LQPSYLQKMT
VLNLLDKMSQ DPQLMVDIFV NYDCDVESSN ILERIVNGLL KTALGPPTGS STTLSPAQDS
TFRNDSVKCL VNLAKAMGNW MDQQLKVNET VWPKGSQVYA SMDSNASQIS ELEGTISDCD
SQPDTSNPEA YDASMLEQRR AYKIELQKGI SLFNRKPSKG VEFLISTKKI GSSPEEVASF
LMKTAGLNGT VIGDYLGERD ELPLKVMHAY VDSFNFEKKD FVEAIRFFLR GFRLPGEAQK
IDRIMEKFAE HYWKCNPGSF TSADTAYVLA YSVIMLNTDA HNNMVKDKMT KADFVRNNRG
IDDGKDLPEE YLGSLYDRVV KEEIRMNSDT LAPQNKQVNG LNKLLGLDGI LNLVSWMQPD
EKPHGANGRL IRDIQEQFQA KPEKSESVYH TVTDISILRF ILEVSWGPML AAFSVTIDQS
DDRLATSLCL QGFRYAVHVT AVMGMQTQRD AFVTSMAKFT NLHCAADMKQ KNVDAVKAII
TIAIEDGNHL HGSWEHILTC LSRIEHLQLL GEVSPSEKRY VPTKKAEVDD KKALGFPNLK
KRGSFQNPSV MAVVRGGSYD STSLVKSVPK LVTPEQIKSF IANLNLLDQI GNFELNHVYA
NSQRLNSEAI VSFVKALCKV SMSELQSPTD PRVFSLTKLV ETAHYNMNRI RLVWSRIWNV
LSDFFVSVGL SENLSVAIFV MDSLRQLSMK FLEREELANY HFQHEFLRPF VVVMQKSSSA
EIRELIVRCV SQMVLSRVSN VKSGWKNVFT VFTTAALDER KNIVLLAFET IEKIVRDHFH
CIIETEITVY ADCIRCLITF TNSKFEGDIG FNTIEFLRFC ALKLEEGGLV LNEKLKNNTI
SALKEDFSDT QSFTDLDEQV SYWIPLLTGL CKQVSDPRPA IRKRSIEVLF HILMDHGHLF
TRPFWTGIFS SIILPVFNNI RSKTDMLFEE SVDSPSSASL DTEETTWDVE TSTLALQLLV
DLLVKFFRSV RSQLPSVVSI IVGFIKSPFQ GSTGSGISVL LHLADGLARS ASEDEWREIF
LALKEAASLT FAGFMKVLRT MDDIEDVETL SGQSVNIGDL DDDSLHIMSY VVSRTKKHID
VLSQIVEVVS DLYRRNQFSL SASHVDILAD IFSCIASHAQ QLNTDTVLRR KFKRACSVQN
LTEPQLLNFE NEAYKSYMMF LQDMVTCNPN VSKELDLESR LVTECAKIVK IYLKCTDPQQ
QEQQQRKPVL WVLPMESDRV EEATARTSLL VSSLEALCSL EAESLKKHVS SFFPLLVDLV
RTEHCSPQVP YVLSNVLKSC IGPILA
