ID   SMBP_NITEU              Reviewed;         117 AA.
AC   Q82S91;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Metal-binding protein SmbP;
DE   Flags: Precursor;
GN   Name=smbP; OrderedLocusNames=NE2461;
OS   Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC
OS   14298).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Nitrosomonadaceae; Nitrosomonas.
OX   NCBI_TaxID=228410;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298;
RX   PubMed=12700255; DOI=10.1128/jb.185.9.2759-2773.2003;
RA   Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L.,
RA   Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A.,
RA   Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.;
RT   "Complete genome sequence of the ammonia-oxidizing bacterium and obligate
RT   chemolithoautotroph Nitrosomonas europaea.";
RL   J. Bacteriol. 185:2759-2773(2003).
RN   [2]
RP   PROTEIN SEQUENCE OF 25-35, CHARACTERIZATION, SUBUNIT, SUBCELLULAR LOCATION,
RP   TRANSCRIPTIONAL REGULATION, AND MASS SPECTROMETRY.
RC   STRAIN=ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298;
RX   PubMed=15366930; DOI=10.1021/bi049318k;
RA   Barney B.M., LoBrutto R., Francisco W.A.;
RT   "Characterization of a small metal binding protein from Nitrosomonas
RT   europaea.";
RL   Biochemistry 43:11206-11213(2004).
CC   -!- FUNCTION: Is capable of binding multiple equivalents of a variety of
CC       divalent and trivalent metals, including Cu(2+) and Fe(3+) but also
CC       Mn(2+), Ni(2+), Mg(2+) and Zn(2+). Is able to bind up to six Cu(2+)
CC       atoms. It is proposed to be a metal scavenging protein that has a role
CC       in cellular copper management in N.europaea.
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:15366930}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:15366930}.
CC   -!- INDUCTION: By copper. {ECO:0000269|PubMed:15366930}.
CC   -!- MASS SPECTROMETRY: Mass=9866; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15366930};
CC   -!- MISCELLANEOUS: There are two separate and distinct copper binding
CC       sites, a high-affinity site and a lower affinity site, which display
CC       negative cooperativity.
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DR   EMBL; AL954747; CAD86373.1; -; Genomic_DNA.
DR   RefSeq; WP_011112924.1; NC_004757.1.
DR   PDB; 3U8V; X-ray; 1.90 A; A/B=25-117.
DR   PDBsum; 3U8V; -.
DR   AlphaFoldDB; Q82S91; -.
DR   SMR; Q82S91; -.
DR   STRING; 228410.NE2461; -.
DR   EnsemblBacteria; CAD86373; CAD86373; NE2461.
DR   KEGG; neu:NE2461; -.
DR   eggNOG; ENOG50334TW; Bacteria.
DR   HOGENOM; CLU_130934_1_1_4; -.
DR   OMA; TKHAQEA; -.
DR   OrthoDB; 2092042at2; -.
DR   Proteomes; UP000001416; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd13840; SMBP_like; 1.
DR   DisProt; DP00205; -.
DR   InterPro; IPR031877; SmbP.
DR   Pfam; PF16785; SMBP; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Copper; Direct protein sequencing; Metal-binding; Periplasm;
KW   Reference proteome; Repeat; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000269|PubMed:15366930"
FT   CHAIN           25..117
FT                   /note="Metal-binding protein SmbP"
FT                   /id="PRO_0000227659"
FT   REPEAT          27..33
FT                   /note="1"
FT   REPEAT          34..40
FT                   /note="2"
FT   REPEAT          41..47
FT                   /note="3"
FT   REPEAT          53..59
FT                   /note="4"
FT   REPEAT          60..66
FT                   /note="5"
FT   REPEAT          72..78
FT                   /note="6"
FT   REPEAT          79..85
FT                   /note="7"
FT   REPEAT          86..92
FT                   /note="8"
FT   REPEAT          98..104
FT                   /note="9"
FT   REPEAT          105..111
FT                   /note="10"
FT   REGION          27..111
FT                   /note="10 X 7 AA approximate repeats"
FT   HELIX           27..46
FT                   /evidence="ECO:0007829|PDB:3U8V"
FT   HELIX           50..67
FT                   /evidence="ECO:0007829|PDB:3U8V"
FT   HELIX           75..91
FT                   /evidence="ECO:0007829|PDB:3U8V"
FT   HELIX           95..111
FT                   /evidence="ECO:0007829|PDB:3U8V"
SQ   SEQUENCE   117 AA;  12448 MW;  1275976B6114414C CRC64;
     MKTTLIKVIA ASVTALFLSM QVYASGHTAH VDEAVKHAEE AVAHGKEGHT DQLLEHAKES
     LTHAKAASEA GGNTHVGHGI KHLEDAIKHG EEGHVGVATK HAQEAIEHLR ASEHKSH