SMBT2_MOUSE
ID SMBT2_MOUSE Reviewed; 938 AA.
AC Q5DTW2; Q80VG7;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 2.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Scm-like with four MBT domains protein 2;
GN Name=Sfmbt2; Synonyms=Kiaa1617;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Fetal brain;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 443-938 (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Ectoplacental cone;
RX PubMed=12466296; DOI=10.1101/gr.670002;
RA Tanaka T.S., Kunath T., Kimber W.L., Jaradat S.A., Stagg C.A., Usuda M.,
RA Yokota T., Niwa H., Rossant J., Ko M.S.;
RT "Gene expression profiling of embryo-derived stem cells reveals candidate
RT genes associated with pluripotency and lineage specificity.";
RL Genome Res. 12:1921-1928(2002).
RN [3]
RP INTERACTION WITH YY1, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, IMPRINTING,
RP AND MISCELLANEOUS.
RX PubMed=18024232; DOI=10.1016/j.modgep.2007.09.005;
RA Kuzmin A., Han Z., Golding M.C., Mann M.R., Latham K.E., Varmuza S.;
RT "The PcG gene Sfmbt2 is paternally expressed in extraembryonic tissues.";
RL Gene Expr. Patterns 8:107-116(2008).
CC -!- FUNCTION: Transcriptional repressor of HOXB13 gene. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with YY1. Interacts with methylated histones H3K9me2
CC and H4K20me2 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5DTW2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5DTW2-2; Sequence=VSP_013858, VSP_013859;
CC -!- TISSUE SPECIFICITY: Expressed in testis and, at much lower levels, in
CC ovary. {ECO:0000269|PubMed:18024232}.
CC -!- DEVELOPMENTAL STAGE: Overall, expression is high in extraembryonic
CC tissues, and low in somatic tissues. Expression starts between 8-cell
CC and blastocyst stages. At 7.5 dpc, highly expressed in the
CC ectoplacental cone and, at much lower levels, in the embryonic and
CC extraembryonic ectoderm. At 14.5 dpc, highly expressed in placenta and,
CC at much lower levels, in the yolk sac. Expression in other tissues,
CC including brain, heart, liver, kidney, gut and genital ridges, is very
CC low. Expressed preferentially from the paternal allele in blastocysts,
CC in various tissues at 7.5 dpc, as well as in yolk sac and placenta at
CC 11.5 and 14.5 dpc. Biallelically expressed in brain and other somatic
CC tissues at 11.5 and 14.5 dpc. {ECO:0000269|PubMed:18024232}.
CC -!- MISCELLANEOUS: Imprinted gene expressed from the paternal allele in
CC blastocysts and in embryonic tissues early in development. Imprinting
CC is lost in somatic tissues after 7.5 dpc.
CC -!- MISCELLANEOUS: [Isoform 2]: Incomplete sequence. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD90456.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK220408; BAD90456.1; ALT_INIT; mRNA.
DR EMBL; AY217748; AAO61788.1; -; mRNA.
DR CCDS; CCDS57152.1; -. [Q5DTW2-2]
DR AlphaFoldDB; Q5DTW2; -.
DR SMR; Q5DTW2; -.
DR STRING; 10090.ENSMUSP00000040575; -.
DR iPTMnet; Q5DTW2; -.
DR PhosphoSitePlus; Q5DTW2; -.
DR MaxQB; Q5DTW2; -.
DR PaxDb; Q5DTW2; -.
DR PRIDE; Q5DTW2; -.
DR ProteomicsDB; 257201; -. [Q5DTW2-1]
DR ProteomicsDB; 257202; -. [Q5DTW2-2]
DR MGI; MGI:2447794; Sfmbt2.
DR eggNOG; KOG3766; Eukaryota.
DR InParanoid; Q5DTW2; -.
DR ChiTaRS; Sfmbt2; mouse.
DR PRO; PR:Q5DTW2; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q5DTW2; protein.
DR GO; GO:0016235; C:aggresome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0042393; F:histone binding; ISS:UniProtKB.
DR GO; GO:0003714; F:transcription corepressor activity; IEA:InterPro.
DR GO; GO:0010629; P:negative regulation of gene expression; ISS:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IBA:GO_Central.
DR CDD; cd09581; SAM_Scm-like-4MBT1_2; 1.
DR Gene3D; 1.10.150.50; -; 1.
DR Gene3D; 3.90.1150.190; -; 1.
DR InterPro; IPR004092; Mbt.
DR InterPro; IPR001660; SAM.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR037604; Scm-like-4MBT1/2_SAM.
DR InterPro; IPR021987; SLED.
DR InterPro; IPR038348; SLED_sf.
DR Pfam; PF02820; MBT; 4.
DR Pfam; PF00536; SAM_1; 1.
DR Pfam; PF12140; SLED; 1.
DR SMART; SM00561; MBT; 4.
DR SMART; SM00454; SAM; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
DR PROSITE; PS51079; MBT; 4.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Reference proteome; Repeat; Repressor.
FT CHAIN 1..938
FT /note="Scm-like with four MBT domains protein 2"
FT /id="PRO_0000071970"
FT REPEAT 43..143
FT /note="MBT 1"
FT REPEAT 151..255
FT /note="MBT 2"
FT REPEAT 265..371
FT /note="MBT 3"
FT REPEAT 379..475
FT /note="MBT 4"
FT DOMAIN 868..931
FT /note="SAM"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 742..836
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..32
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 762..778
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 805..823
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 661
FT /note="Y -> YNKREKGSSLSPASELKDDSASHATKLQKAKIPQ (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:12466296"
FT /id="VSP_013858"
FT VAR_SEQ 751
FT /note="E -> ESLPE (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12466296"
FT /id="VSP_013859"
SQ SEQUENCE 938 AA; 105981 MW; 7D5C7532DD678C33 CRC64;
MERYLPVSKK RNSSSSLEKI TGSANGNGTL YSEEDTNLEE NDFSWGDYLE ETGTRAVPHV
SFRHVEISIR SNFQPGMKLE VANKNNPDTY WVATIITTCG QLLLLRYCGY GEDRRADFWC
DVIIADLHPV GWCTQNNKVL RPPDAIKDKY SDWTDFLIRE LTGSRTAPAN LLEGPLRGKG
PIDLITVDSL IELQDSQNPF QYWIVSVTEN VGGRLRLRYV GLEHTESYDR WLFYLDYRLR
PIGWCQENKY RMDPPSDLYY LKLPFEWKCA LEKALVLAAE SPLPMEVFKD HADLQSHFFT
VGMRLETLHI SDPFHIYPAS VTKVFNSKFF QVAIDDLRPE ADKPTMLCHA DSLGILPVQW
CLKNGVNLAP PKGYSGQDFN WVDYHKQRQA EEAPHFCFKN AFSRGFSKNM KLEAVNPVNP
GEVCVATVVS VKGRLLWLHL EGLETPMPDI IVDMDSMDIF PVGWCEANSY PLTTPYKASS
KSKRKTVHFK MEKQLLSPVP IEKIPHELCL LPPQMDSPVG AINAKYCCPQ LFVNHRCFSG
PFLNKGRISE LPQSVGPGMC VLVLKEILTL ITNAAYKPGR VLRELQLVED PEWNSQEEIL
KAKYGGKTYR AVVKIVRTAD QVMNFCRQVC AKLECCPNLL SPVLISETCP ENCSVHTKTR
YTYYYGKRRR VIQSSLRVSN IETPPKSTRR RKRRKSVYVQ KRRKSAIVVP AGVPAGVPAG
VPEDIPAGIP EGIPASIPES IPEGIPESLP EAIPESIPKG SAQKTEQEKR ETLDTARKKT
GYHGPAYQTD TSAAQVPFAR PRRAVTLRRN SEALKRPPVE RARRVRTVPT TASSNNRVKG
PLVRIVKPED SSQSDEEKLI LESNPLEWSV TDVVRFIKLT DCAPLARIFQ EQDIDGQALL
LLTLPTVQEC MELKLGPAIK LCHQIERVKV AFYAQYAS
