SMB_ARATH
ID SMB_ARATH Reviewed; 371 AA.
AC Q9MA17;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Protein SOMBRERO;
DE AltName: Full=NAC domain-containing protein 33;
DE Short=ANAC033;
GN Name=SMB; Synonyms=NAC033; OrderedLocusNames=At1g79580; ORFNames=F20B17.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION BY FEZ,
RP DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF ARG-22 AND
RP GLU-27.
RX PubMed=19081078; DOI=10.1016/j.devcel.2008.09.019;
RA Willemsen V., Bauch M., Bennett T., Campilho A., Wolkenfelt H., Xu J.,
RA Haseloff J., Scheres B.;
RT "The NAC domain transcription factors FEZ and SOMBRERO control the
RT orientation of cell division plane in Arabidopsis root stem cells.";
RL Dev. Cell 15:913-922(2008).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=20197506; DOI=10.1105/tpc.109.072272;
RA Bennett T., van den Toorn A., Sanchez-Perez G.F., Campilho A.,
RA Willemsen V., Snel B., Scheres B.;
RT "SOMBRERO, BEARSKIN1, and BEARSKIN2 regulate root cap maturation in
RT Arabidopsis.";
RL Plant Cell 22:640-654(2010).
CC -!- FUNCTION: Transcription regulator. Together with BRN1 and BRN2,
CC regulates cellular maturation of root cap. Represses stem cell-like
CC divisions in the root cap daughter cells, and thus promotes daughter
CC cell fate. Inhibits expression of its positive regulator FEZ in a
CC feedback loop for controlled switches in cell division plane. Promotes
CC the expression of genes involved in secondary cell walls (SCW)
CC biosynthesis. {ECO:0000269|PubMed:19081078,
CC ECO:0000269|PubMed:20197506}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353,
CC ECO:0000269|PubMed:19081078}.
CC -!- TISSUE SPECIFICITY: Accumulates in maturing root cap cells, in both COL
CC and LRC cells. {ECO:0000269|PubMed:19081078,
CC ECO:0000269|PubMed:20197506}.
CC -!- DEVELOPMENTAL STAGE: First expressed at early heart stage onward in all
CC root basal daughter cells resulting from horizontal divisions in the
CC COL progenitors and is later maintained in these cells. Present in root
CC stem cell daughters and accumulates in maturing root cap layers.
CC Detectable from very early stages of lateral root development.
CC {ECO:0000269|PubMed:19081078, ECO:0000269|PubMed:20197506}.
CC -!- INDUCTION: By FEZ in oriented-divised root cap stem cells.
CC {ECO:0000269|PubMed:19081078}.
CC -!- DOMAIN: The NAC domain includes a DNA-binding domain and a dimerization
CC domain.
CC -!- DISRUPTION PHENOTYPE: Additional root cap layers; increased number of
CC columella (COL) and lateral root cap (LRC) cell layers in the mature
CC embryo and in the postembryonic state. Lateral cap cells continue to
CC divide and fail to detach from the root. {ECO:0000269|PubMed:19081078,
CC ECO:0000269|PubMed:20197506}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BX816756; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC010793; AAF68129.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36266.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36267.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36268.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM61091.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM61092.1; -; Genomic_DNA.
DR EMBL; BX816756; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_001319416.1; NM_001334893.1.
DR RefSeq; NP_001323331.1; NM_001334894.1.
DR RefSeq; NP_178076.1; NM_106606.2.
DR RefSeq; NP_974178.1; NM_202449.2.
DR RefSeq; NP_974179.1; NM_202450.2.
DR AlphaFoldDB; Q9MA17; -.
DR SMR; Q9MA17; -.
DR BioGRID; 29515; 1.
DR IntAct; Q9MA17; 1.
DR STRING; 3702.AT1G79580.1; -.
DR PaxDb; Q9MA17; -.
DR PRIDE; Q9MA17; -.
DR ProteomicsDB; 226744; -.
DR EnsemblPlants; AT1G79580.1; AT1G79580.1; AT1G79580.
DR EnsemblPlants; AT1G79580.2; AT1G79580.2; AT1G79580.
DR EnsemblPlants; AT1G79580.3; AT1G79580.3; AT1G79580.
DR EnsemblPlants; AT1G79580.4; AT1G79580.4; AT1G79580.
DR EnsemblPlants; AT1G79580.5; AT1G79580.5; AT1G79580.
DR GeneID; 844296; -.
DR Gramene; AT1G79580.1; AT1G79580.1; AT1G79580.
DR Gramene; AT1G79580.2; AT1G79580.2; AT1G79580.
DR Gramene; AT1G79580.3; AT1G79580.3; AT1G79580.
DR Gramene; AT1G79580.4; AT1G79580.4; AT1G79580.
DR Gramene; AT1G79580.5; AT1G79580.5; AT1G79580.
DR KEGG; ath:AT1G79580; -.
DR Araport; AT1G79580; -.
DR TAIR; locus:2019833; AT1G79580.
DR eggNOG; ENOG502QT1N; Eukaryota.
DR HOGENOM; CLU_035664_1_4_1; -.
DR InParanoid; Q9MA17; -.
DR OMA; TMMINDM; -.
DR OrthoDB; 1140648at2759; -.
DR PhylomeDB; Q9MA17; -.
DR PRO; PR:Q9MA17; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9MA17; baseline and differential.
DR Genevisible; Q9MA17; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0009834; P:plant-type secondary cell wall biogenesis; IMP:UniProtKB.
DR GO; GO:0010455; P:positive regulation of cell fate commitment; IMP:TAIR.
DR GO; GO:0003002; P:regionalization; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0048829; P:root cap development; IMP:UniProtKB.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR InterPro; IPR033518; SMB.
DR PANTHER; PTHR31989:SF297; PTHR31989:SF297; 1.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..371
FT /note="Protein SOMBRERO"
FT /id="PRO_0000394192"
FT DOMAIN 17..166
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 118..172
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT REGION 176..213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 316..355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 176..203
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 22
FT /note="R->W: Additional root cap layers."
FT /evidence="ECO:0000269|PubMed:19081078"
FT MUTAGEN 27
FT /note="E->K: Additional root cap layers."
FT /evidence="ECO:0000269|PubMed:19081078"
SQ SEQUENCE 371 AA; 42382 MW; 746F5C6122DFBAFD CRC64;
MEIGSSSTVA GGGQLSVPPG FRFHPTEEEL LYYYLKKKVS YEPIDLDVIR EVDLNKLEPW
ELKEKCRIGS GPQNEWYFFS HKDKKYPTGT RTNRATAAGF WKATGRDKSI HLNSSKKIGL
RKTLVFYTGR APHGQKTEWI MHEYRLDDSE NEIQEDGWVV CRVFKKKNHF RGFHQEQEQD
HHHHHQYIST NNDHDHHHHI DSNSNNHSPL ILHPLDHHHH HHHIGRQIHM PLHEFANTLS
HGSMHLPQLF SPDSAAAAAA AAASAQPFVS PINTTDIECS QNLLRLTSNN NYGGDWSFLD
KLLTTGNMNQ QQQQQVQNHQ AKCFGDLSNN DNNDQADHLG NNNGGSSSSP VNQRFPFHYL
GNDANLLKFP K
