ID   BIGR_AGRFC              Reviewed;         117 AA.
AC   Q8UAA8; Q7CSJ3;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 3.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Biofilm growth-associated repressor;
GN   Name=bigR; OrderedLocusNames=Atu3466; ORFNames=AGR_L_2725;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
RN   [3]
RP   FUNCTION AS A REPRESSOR AND IN BIOFILM FORMATION, AND DISRUPTION PHENOTYPE.
RX   PubMed=17586627; DOI=10.1128/jb.00331-07;
RA   Barbosa R.L., Benedetti C.E.;
RT   "BigR, a transcriptional repressor from plant-associated bacteria,
RT   regulates an operon implicated in biofilm growth.";
RL   J. Bacteriol. 189:6185-6194(2007).
CC   -!- FUNCTION: Represses an operon that comprises at least itself and blh.
CC       Binds to a palindromic AT-rich sequence spanning the -10 region of the
CC       blh promoter and blocks transcription of the operon.
CC       {ECO:0000269|PubMed:17586627}.
CC   -!- DISRUPTION PHENOTYPE: Cells show altered biofilm formation; on glass
CC       surfaces and on tobacco root surfaces, it exhibits a greater biofilm
CC       mass than the wild-type. {ECO:0000269|PubMed:17586627}.
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DR   EMBL; AE007870; AAK89928.1; -; Genomic_DNA.
DR   PIR; AI2982; AI2982.
DR   PIR; F98300; F98300.
DR   RefSeq; NP_357143.1; NC_003063.2.
DR   RefSeq; WP_010973063.1; NC_003063.2.
DR   AlphaFoldDB; Q8UAA8; -.
DR   SMR; Q8UAA8; -.
DR   STRING; 176299.Atu3466; -.
DR   EnsemblBacteria; AAK89928; AAK89928; Atu3466.
DR   GeneID; 66223768; -.
DR   KEGG; atu:Atu3466; -.
DR   PATRIC; fig|176299.10.peg.3305; -.
DR   eggNOG; COG0640; Bacteria.
DR   HOGENOM; CLU_097806_6_4_5; -.
DR   OMA; TIWYSIA; -.
DR   PhylomeDB; Q8UAA8; -.
DR   Proteomes; UP000000813; Chromosome linear.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   CDD; cd00090; HTH_ARSR; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011991; ArsR-like_HTH.
DR   InterPro; IPR001845; HTH_ArsR_DNA-bd_dom.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PRINTS; PR00778; HTHARSR.
DR   SMART; SM00418; HTH_ARSR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50987; HTH_ARSR_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..117
FT                   /note="Biofilm growth-associated repressor"
FT                   /id="PRO_0000305335"
FT   DOMAIN          20..114
FT                   /note="HTH arsR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   DNA_BIND        54..77
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
SQ   SEQUENCE   117 AA;  12986 MW;  A8B313FCA39C191C CRC64;
     MVTETPLEKP LDIGEIPLPA MEKRATEVAI LLKTLAHPAR LMLACTLAQG EFSVGELEAK
     LDIRQPTLSQ QLGVLREAGI VDTRREAKQI FYRLAEDKAA RLIEALYAIF CAPEENL