BIGR_XYLFA
ID BIGR_XYLFA Reviewed; 114 AA.
AC Q9PFB1;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=Biofilm growth-associated repressor;
GN Name=bigR; OrderedLocusNames=XF_0767;
OS Xylella fastidiosa (strain 9a5c).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xylella.
OX NCBI_TaxID=160492;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=9a5c;
RX PubMed=10910347; DOI=10.1038/35018003;
RA Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M.,
RA Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S.,
RA Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S.,
RA Bueno M.R.P., Camargo A.A., Camargo L.E.A., Carraro D.M., Carrer H.,
RA Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M.,
RA Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H.,
RA Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., Fraga J.S.,
RA Franca S.C., Franco M.C., Frohme M., Furlan L.R., Garnier M., Goldman G.H.,
RA Goldman M.H.S., Gomes S.L., Gruber A., Ho P.L., Hoheisel J.D.,
RA Junqueira M.L., Kemper E.L., Kitajima J.P., Krieger J.E., Kuramae E.E.,
RA Laigret F., Lambais M.R., Leite L.C.C., Lemos E.G.M., Lemos M.V.F.,
RA Lopes S.A., Lopes C.R., Machado J.A., Machado M.A., Madeira A.M.B.N.,
RA Madeira H.M.F., Marino C.L., Marques M.V., Martins E.A.L., Martins E.M.F.,
RA Matsukuma A.Y., Menck C.F.M., Miracca E.C., Miyaki C.Y.,
RA Monteiro-Vitorello C.B., Moon D.H., Nagai M.A., Nascimento A.L.T.O.,
RA Netto L.E.S., Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A.,
RA de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., Peixoto B.R.,
RA Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., Quaggio R.B.,
RA Roberto P.G., Rodrigues V., de Rosa A.J.M., de Rosa V.E. Jr., de Sa R.G.,
RA Santelli R.V., Sawasaki H.E., da Silva A.C.R., da Silva A.M.,
RA da Silva F.R., Silva W.A. Jr., da Silveira J.F., Silvestri M.L.Z.,
RA Siqueira W.J., de Souza A.A., de Souza A.P., Terenzi M.F., Truffi D.,
RA Tsai S.M., Tsuhako M.H., Vallada H., Van Sluys M.A., Verjovski-Almeida S.,
RA Vettore A.L., Zago M.A., Zatz M., Meidanis J., Setubal J.C.;
RT "The genome sequence of the plant pathogen Xylella fastidiosa.";
RL Nature 406:151-159(2000).
RN [2]
RP FUNCTION AS A REPRESSOR.
RC STRAIN=9a5c;
RX PubMed=17586627; DOI=10.1128/jb.00331-07;
RA Barbosa R.L., Benedetti C.E.;
RT "BigR, a transcriptional repressor from plant-associated bacteria,
RT regulates an operon implicated in biofilm growth.";
RL J. Bacteriol. 189:6185-6194(2007).
CC -!- FUNCTION: Represses an operon that comprises itself, XF_0764, XF_0765,
CC XF_0766 and blh. Binds to a palindromic AT-rich sequence spanning the
CC -10 region of the blh promoter and blocks transcription of the operon.
CC {ECO:0000269|PubMed:17586627}.
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DR EMBL; AE003849; AAF83577.1; -; Genomic_DNA.
DR PIR; D82766; D82766.
DR RefSeq; WP_010893290.1; NC_002488.3.
DR PDB; 3PQJ; X-ray; 2.48 A; A/B/C/D=13-114.
DR PDB; 3PQK; X-ray; 2.09 A; A/B/C/D/E/F=13-114.
DR PDBsum; 3PQJ; -.
DR PDBsum; 3PQK; -.
DR AlphaFoldDB; Q9PFB1; -.
DR SMR; Q9PFB1; -.
DR STRING; 160492.XF_0767; -.
DR PRIDE; Q9PFB1; -.
DR EnsemblBacteria; AAF83577; AAF83577; XF_0767.
DR KEGG; xfa:XF_0767; -.
DR eggNOG; COG0640; Bacteria.
DR HOGENOM; CLU_097806_6_4_6; -.
DR OMA; TIWYSIA; -.
DR EvolutionaryTrace; Q9PFB1; -.
DR Proteomes; UP000000812; Chromosome.
DR CollecTF; EXPREG_000017b0; -.
DR GO; GO:0032993; C:protein-DNA complex; IPI:CollecTF.
DR GO; GO:0001217; F:DNA-binding transcription repressor activity; IPI:CollecTF.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:CollecTF.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEP:CollecTF.
DR CDD; cd00090; HTH_ARSR; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011991; ArsR-like_HTH.
DR InterPro; IPR001845; HTH_ArsR_DNA-bd_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PRINTS; PR00778; HTHARSR.
DR SMART; SM00418; HTH_ARSR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50987; HTH_ARSR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..114
FT /note="Biofilm growth-associated repressor"
FT /id="PRO_0000305336"
FT DOMAIN 17..111
FT /note="HTH arsR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT DNA_BIND 51..74
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT HELIX 17..32
FT /evidence="ECO:0007829|PDB:3PQK"
FT HELIX 35..45
FT /evidence="ECO:0007829|PDB:3PQK"
FT HELIX 51..58
FT /evidence="ECO:0007829|PDB:3PQK"
FT HELIX 64..74
FT /evidence="ECO:0007829|PDB:3PQK"
FT STRAND 77..81
FT /evidence="ECO:0007829|PDB:3PQK"
FT STRAND 83..85
FT /evidence="ECO:0007829|PDB:3PQK"
FT STRAND 88..91
FT /evidence="ECO:0007829|PDB:3PQK"
FT HELIX 96..106
FT /evidence="ECO:0007829|PDB:3PQK"
FT HELIX 108..111
FT /evidence="ECO:0007829|PDB:3PQK"
SQ SEQUENCE 114 AA; 12989 MW; 3AB9B2C6C5A58CF7 CRC64;
MVNEMRDDTR PHMTREDMEK RANEVANLLK TLSHPVRLML VCTLVEGEFS VGELEQQIGI
GQPTLSQQLG VLRESGIVET RRNIKQIFYR LTEAKAAQLV NALYTIFCAQ EKQA
