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SMC6_CAEEL
ID   SMC6_CAEEL              Reviewed;        1149 AA.
AC   G5EG17;
DT   12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Structural maintenance of chromosomes protein 6 homolog smc-6 {ECO:0000305};
GN   Name=smc-6 {ECO:0000312|WormBase:F54D5.14};
GN   ORFNames=F54D5.14 {ECO:0000312|WormBase:F54D5.14};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, INTERACTION WITH SMC-5, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP   AND DISRUPTION PHENOTYPE.
RX   PubMed=20661436; DOI=10.1371/journal.pgen.1001028;
RA   Bickel J.S., Chen L., Hayward J., Yeap S.L., Alkers A.E., Chan R.C.;
RT   "Structural maintenance of chromosomes (SMC) proteins promote homolog-
RT   independent recombination repair in meiosis crucial for germ cell genomic
RT   stability.";
RL   PLoS Genet. 6:E1001028-E1001028(2010).
RN   [3] {ECO:0000305}
RP   FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=24424777; DOI=10.1534/genetics.113.158295;
RA   Wolters S., Ermolaeva M.A., Bickel J.S., Fingerhut J.M., Khanikar J.,
RA   Chan R.C., Schumacher B.;
RT   "Loss of Caenorhabditis elegans BRCA1 promotes genome stability during
RT   replication in smc-5 mutants.";
RL   Genetics 196:985-999(2014).
RN   [4] {ECO:0000305}
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=24939994; DOI=10.1534/genetics.114.164152;
RA   Checchi P.M., Lawrence K.S., Van M.V., Larson B.J., Engebrecht J.;
RT   "Pseudosynapsis and decreased stringency of meiotic repair pathway choice
RT   on the hemizygous sex chromosome of Caenorhabditis elegans males.";
RL   Genetics 197:543-560(2014).
RN   [5] {ECO:0000305}
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=27010650; DOI=10.1371/journal.pgen.1005872;
RA   Hong Y., Sonneville R., Agostinho A., Meier B., Wang B., Blow J.J.,
RA   Gartner A.;
RT   "The SMC-5/6 complex and the HIM-6 (BLM) helicase synergistically promote
RT   meiotic recombination intermediate processing and chromosome maturation
RT   during Caenorhabditis elegans meiosis.";
RL   PLoS Genet. 12:E1005872-E1005872(2016).
CC   -!- FUNCTION: Core component of the smc-5/smc-6 complex (PubMed:20661436).
CC       Involved in DNA double-strand break repair by promoting sister-
CC       chromatid homologous recombination during meiosis (PubMed:20661436,
CC       PubMed:24939994, PubMed:27010650). Also plays a role in the DNA damage
CC       repair of ultraviolet (UV) radiation-induced DNA lesions
CC       (PubMed:24424777). Promotes efficient DNA replication
CC       (PubMed:24424777). {ECO:0000269|PubMed:20661436,
CC       ECO:0000269|PubMed:24424777, ECO:0000269|PubMed:24939994,
CC       ECO:0000269|PubMed:27010650}.
CC   -!- SUBUNIT: Interacts with smc-5. {ECO:0000269|PubMed:20661436}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20661436}. Chromosome
CC       {ECO:0000269|PubMed:20661436}. Note=Nucleoplasmic in the distal gonad
CC       arm and localizes on chromosomes during pachytene, diplotene and
CC       diakinesis in oocytes. {ECO:0000269|PubMed:20661436}.
CC   -!- TISSUE SPECIFICITY: Expressed in the germline (at protein level).
CC       {ECO:0000269|PubMed:20661436}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the primordial germ cells Z2 and Z3
CC       in embryos and in proliferating germ cells in L2/L3 stage larvae (at
CC       protein level). {ECO:0000269|PubMed:24424777}.
CC   -!- DOMAIN: The flexible hinge domain, which separates the large
CC       intramolecular coiled coil regions, allows the heterotypic interaction
CC       with the corresponding domain of smc-5, forming a V-shaped heterodimer.
CC       {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Reduced fecundity and transgenerational sterility
CC       (PubMed:20661436). Increased germ cell apoptosis and hypersensitivity
CC       to ultraviolet and ionizing radiation and hydroxyurea-induced
CC       replication stress (PubMed:20661436, PubMed:24424777). Increased rad-51
CC       foci in the mitotic and meiotic germline on autosomes, but not on X
CC       chromosomes (PubMed:20661436, PubMed:27010650, PubMed:24424777,
CC       PubMed:24939994). Chromosome fragmentation defects and unresolved
CC       diakinesis chromosomes in meiotic oocytes (PubMed:20661436,
CC       PubMed:27010650). Impaired DNA replication in germ cells
CC       (PubMed:24424777). Accumulation of brd-1 on chromosomes in mitotic germ
CC       cells (PubMed:24424777). In a him-3 mutant background, increased rad-51
CC       foci and chromosome fragmentation (PubMed:20661436). RNAi-mediated
CC       knockdown of syp-2 leads to an increase of chromosome fragmentation
CC       (PubMed:20661436). In a him-6 mutant background, leads to synergistic
CC       lethality (PubMed:27010650). In a fem-3 or him-8 mutant background,
CC       RNAi-mediated knockdown leads to increased fragmentation of autosomes
CC       and asynapsed X chromosomes but not of X chromosomes in males
CC       (PubMed:24939994). {ECO:0000269|PubMed:20661436,
CC       ECO:0000269|PubMed:24424777, ECO:0000269|PubMed:24939994,
CC       ECO:0000269|PubMed:27010650}.
CC   -!- SIMILARITY: Belongs to the SMC family. SMC6 subfamily. {ECO:0000305}.
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DR   EMBL; BX284602; CAA91339.2; -; Genomic_DNA.
DR   RefSeq; NP_496476.2; NM_064075.6.
DR   AlphaFoldDB; G5EG17; -.
DR   SMR; G5EG17; -.
DR   STRING; 6239.F54D5.14; -.
DR   EPD; G5EG17; -.
DR   PaxDb; G5EG17; -.
DR   PeptideAtlas; G5EG17; -.
DR   EnsemblMetazoa; F54D5.14.1; F54D5.14.1; WBGene00010056.
DR   GeneID; 174776; -.
DR   KEGG; cel:CELE_F54D5.14; -.
DR   CTD; 174776; -.
DR   WormBase; F54D5.14; CE46466; WBGene00010056; smc-6.
DR   eggNOG; KOG0250; Eukaryota.
DR   GeneTree; ENSGT00550000074816; -.
DR   HOGENOM; CLU_009063_0_0_1; -.
DR   InParanoid; G5EG17; -.
DR   OMA; MCHDHFY; -.
DR   OrthoDB; 263113at2759; -.
DR   PhylomeDB; G5EG17; -.
DR   Reactome; R-CEL-3108214; SUMOylation of DNA damage response and repair proteins.
DR   PRO; PR:G5EG17; -.
DR   Proteomes; UP000001940; Chromosome II.
DR   Bgee; WBGene00010056; Expressed in adult organism and 4 other tissues.
DR   GO; GO:0000794; C:condensed nuclear chromosome; IDA:WormBase.
DR   GO; GO:0005654; C:nucleoplasm; IDA:WormBase.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0035861; C:site of double-strand break; IBA:GO_Central.
DR   GO; GO:0030915; C:Smc5-Smc6 complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0003684; F:damaged DNA binding; IBA:GO_Central.
DR   GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR   GO; GO:0051276; P:chromosome organization; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR003395; RecF/RecN/SMC_N.
DR   InterPro; IPR027132; SMC6.
DR   InterPro; IPR036277; SMC_hinge_sf.
DR   PANTHER; PTHR19306:SF6; PTHR19306:SF6; 1.
DR   Pfam; PF02463; SMC_N; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF75553; SSF75553; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Chromosome; Coiled coil; DNA damage; DNA recombination;
KW   DNA repair; DNA replication; Meiosis; Nucleotide-binding; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..1149
FT                   /note="Structural maintenance of chromosomes protein 6
FT                   homolog smc-6"
FT                   /id="PRO_0000439583"
FT   REGION          461..687
FT                   /note="Flexible hinge"
FT                   /evidence="ECO:0000305"
FT   REGION          875..900
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1026..1060
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          309..460
FT                   /evidence="ECO:0000255"
FT   COILED          714..920
FT                   /evidence="ECO:0000255"
FT   BINDING         77..84
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00499"
SQ   SEQUENCE   1149 AA;  131796 MW;  D8C46D9EBC068EB4 CRC64;
     MGKRDIPSPN ENIAPRTIGM RDVDLTAAPA KKAKLDTGER IAVSGRVASV KLTNFMCHAN
     LQIDFKTAQN NCFYIGGPNG SGKSALFAAI NLGLGGRGSD NDRGNTVKSY IKDGTTQSKI
     TITLTNAGLN AHPDFDDLIS IERTINQASS TYIMKSVKVT SSDNHVERIV SRKKADVDRI
     VSRFSIHLSN PAFWMSQDRS RSFLANFKPA NVYKLYLEST NLENIRLSYI RFADALDECF
     ALIQLKAGEI LNEQKKLKRM QEQRDLQAKL DQDRALVASF CWKLLFCKVR DYNDQIELTL
     KKQEAQKTLQ DETKKEYAKN RAARTEVEKK IQEFRDEVEV QDAEIAEARE DLDAKKRKVL
     EFEEKIRECE QSIRKKTSEK KYMERTIVNA KNEVRILLEK QGNQDLTKRL TKVENDYKDI
     SQQRENMELG GESAKLREKL DTVITDYKRK EEEKYTIQRD INQLRRKIEQ DMETMRRSRA
     TKKDAINKFG SHMAEILMEI NRSKSRFQTV PKGPLGKYIT LIDPKWAFTV EECIGNLANN
     FLCSSHLDAE ILRNIFQSLR IPAQDRPTII VAKCNGRAYT NLHEPSSDFK SIYRVLKFSD
     PDVHNVIIDK SNCEQFILIE DKTEAMELMG SNYPPQNAVK AYTLDGSQAY ANGPNSQYRF
     YSGRGGHARG TFGNDQGDVD EGALARLIED TKSEAMRLET QDLRKQDHEL KVIYNERDQT
     KAAIDEFDRK LSNLRSQELQ KERQAKDLRA ELAQTANEDQ VENLNESIEE MQKKIPLIED
     EVKDILKNVA DITADMAPVI QERKEAEHTL AEIQKETRDF ASKSQKLQNE LSKYDDAGEI
     LKIRLDKVKA DEGVFFHTEA KLKSERDDAM EMVENDKKNH PMPPGETDPP DLSSFPSTTE
     AQRKIEEMQK AVDRATVGCD TTITLECVKD FKDKLKRLKY LCRMIEDVLI ELKNLHAARV
     KAYPSLKKFT ELKVCNKFQE LLAVRGHFIG GLEFDHEKET LNVNVQSSKE KDAMAGRRPE
     VLEVEEVDEH SYDDDSDDST GPRRKKSKKS GQKKKRVRDL KGLSGGERSF VTAALVMSLW
     EVMEQPFRMM DEFDVFMDMM NRKLVMDLLV ELATKKFPHN QFIFFTPQGI KELNMVDGLQ
     VFEMNRVRD
 
 
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