BIG_ORYSJ
ID BIG_ORYSJ Reviewed; 4965 AA.
AC B9G2A8; Q6K4G1;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Auxin transport protein BIG;
GN OrderedLocusNames=Os09g0247700, LOC_Os09g07294;
GN ORFNames=OJ1451_A02.25, OsJ_28507, P0499G10.6;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Required for auxin efflux and polar auxin transport (PAT)
CC influencing auxin-mediated developmental responses (e.g. cell
CC elongation, apical dominance, lateral root production, inflorescence
CC architecture, general growth and development) (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the UBR4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD23392.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD25995.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF24578.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP005571; BAD23392.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP005587; BAD25995.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008215; BAF24578.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014965; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CM000146; EEE69254.1; -; Genomic_DNA.
DR STRING; 39947.B9G2A8; -.
DR PaxDb; B9G2A8; -.
DR PRIDE; B9G2A8; -.
DR eggNOG; KOG1776; Eukaryota.
DR HOGENOM; CLU_2874586_0_0_1; -.
DR InParanoid; B9G2A8; -.
DR Proteomes; UP000000763; Chromosome 9.
DR Proteomes; UP000007752; Chromosome 9.
DR Proteomes; UP000059680; Chromosome 9.
DR Genevisible; B9G2A8; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0009926; P:auxin polar transport; ISS:UniProtKB.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009826; P:unidimensional cell growth; ISS:UniProtKB.
DR Gene3D; 3.30.60.90; -; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR025704; E3_Ub_ligase_UBR4_C.
DR InterPro; IPR045189; UBR4-like.
DR InterPro; IPR003126; Znf_UBR.
DR InterPro; IPR000433; Znf_ZZ.
DR InterPro; IPR043145; Znf_ZZ_sf.
DR PANTHER; PTHR21725; PTHR21725; 1.
DR Pfam; PF13764; E3_UbLigase_R4; 1.
DR Pfam; PF00569; ZZ; 1.
DR SMART; SM00396; ZnF_UBR1; 1.
DR SMART; SM00291; ZnF_ZZ; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS51157; ZF_UBR; 1.
DR PROSITE; PS01357; ZF_ZZ_1; 1.
DR PROSITE; PS50135; ZF_ZZ_2; 1.
PE 2: Evidence at transcript level;
KW Auxin signaling pathway; Developmental protein; Membrane; Metal-binding;
KW Reference proteome; Transmembrane; Transmembrane helix; Zinc; Zinc-finger.
FT CHAIN 1..4965
FT /note="Auxin transport protein BIG"
FT /id="PRO_0000410728"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 646..666
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 772..792
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ZN_FING 1431..1502
FT /note="UBR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00508"
FT ZN_FING 2469..2528
FT /note="ZZ-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT ZN_FING 3319..3359
FT /note="MYND-type; degenerate"
FT REGION 1383..1425
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2437..2456
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2997..3037
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3672..3721
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4753..4778
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4822..4846
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1395..1418
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2997..3015
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3016..3034
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3689..3716
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4825..4846
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 2474
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 2477
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 2489
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 2492
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 2498
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 2501
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 2514
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
FT BINDING 2518
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00228"
SQ SEQUENCE 4965 AA; 552028 MW; 24E05E512D8EB9E9 CRC64;
MSIMSSINEC LATCKVLISP SMLPLHVLLS VEQVESTVVE IVERSLEFCL LYLEKSSYAC
EDYGLLNEVA YFMECVLLRG TPSKVYSLEP SVVNDVIEQW SSVQVDSERI SPQEKYFCYL
KGFNCSNSGD DLQRFRLTLS PECLQQDYVI AENTESSHTA SPNGMVSIAQ HFAVVHLHCI
PVLLTLVQKL CQSPALDVIE DTNFNMRLSF GQRILKLVHG LAMEFPCDAS DAMMLCSVAR
CTDSLPVLFK LKFKFANHDR VFSGDGVGTV LLQILDEFLQ LIHIIFCNSD ICCTVQVCIL
ASLLEIFSPE KWKYDRSAAC LMPPLVYSPH IVQYVLKLLN DTKRWTSRVD RDRPGKDVLG
YSCNSETDGL SCHARSKKVP LLKKYTSEEY LQLIFPSEEQ WLDDLVHLIF FLHEEGVKSM
PLLEKPQMSC TKQVTLSELE SVASHEEEAL FGNLFAEARS TGVADSVEQP ISLGSGPSSS
QHGPIQLAAD LICFMKMSIF SPEWCTAIYV DACRKFHSNH LEQFLSILQC PAFCSDESIA
TTSLSEVNSL HINTACFELL QMFLISHECP ASLREDLVDK VFNAENGMYT YNNYTLALVA
RAIISGASSI YNLGRKVFVQ ENETASTWSK CIWTHKMGFT PVKTPACLAA YVVVSGNTSV
MVAYEVKIQN EGDILLKEGQ SRSMNDYLPS FTAEVVEGIF ADTVKEYAST SSLFPQLIDV
TPAHAEIYFD KSALEALGLN FANLGSNISE ILGVWKGRKA EVAEDLIAER YLFLICWSTL
SGIGYSGGYE GLLNPDFADV NFFISFALSV SDDASSLLDA NLPSVIFGFL KLLQSEILCG
PSVLESWDFL RKGAWLSLIL SLINTGFWGH QTSGKPDVDL QGKQVVQDAE IFGKSLLTFI
SENSGHCLHV LSSLLETYLH AFKEAFISFV VEKGRVCEDH CYPSWLLKHS AFDKSKHPLL
FEKVGSNIGM LEPICDLSSR IDRVATKLGD GRKEYFLLKC LLHGFPVNSA SNNSAILSCV
LVINEIIYML NGCIKIMQPN DRDLVDVGVI SKLLSMIMTI KSDGMFTSIH KLCDSIFMSL
IDQKDDLAGY SDLFVLKQLE GYLADINSKE IMDNEVKEII VFTIVDLVED LRSKTNVFKF
FLGEAEGAPE RANSLFALEQ ADMSVFIDVL DKCQSEQVNL KILNLFTDIL GDGLCPDLKQ
KLQHKFIGMD VSCFSSWLEF RTLGHSMKIE STNSTTSGPT ALRELTMDFL MRLTCPSSET
LAKELQHHLF DSMLLLLDKA FMSCDLQIVK AHFHFIAQLS TDESHFKELF EKTLKLMENM
VGNEGLLHTL KFLFTCVESV FGDAGSNRSA LKRLSSKSSG NSFGSGSLIP KQLKNSDSLV
LRTNQESNST VDCDASSGEE DEDDGTSDGE LVSIDRDEEE DGNSERALAT KVCTFTSSGS
NFMEQHWYFC YTCDLTVSKG CCSVCAKVCH QGHRVVYSRS SRFFCDCGAG GVRGSSCQCL
KPRKFTGTSS VSPPVTSSFQ PILPYHEDVE PVADSGSDFE DDISTEAENC IKLSVPKGFS
DELPVFLKNL DVEVRMLELC KKLLPMILSQ RELNLLKDRK VFLGGEMPMS QASDIFQLKK
AFKSGSLDLK IKADYPNSRE LKSHLANGSL TKSLLSISIR GKLAVGEGDK VAIFDVGQII
GQPTAAPITA DKTNVKPLSR NIVRFEIVHL IFNPLVEHYL SVAGYEDCQV LTLNSRGEVT
DRLAIELALQ GAYIRCVEWV PGSQVQLMVV TNKFVKIYDL SQDNISPLHY FTVADDIIVD
ATLVPSSMGK LVLLVLSEGG LLYRLNVALA GDVGAKTLTD TVLVKDAVSM HKGLSLYFSS
TYRLLFVSHQ DGTTYMGRLD GDSSSITELS YICENDQDGK SKPAGLYRWR ELIAGSGALA
CLSKFKSNSP LAVSLGPHEL FAHNMRHASG SNAPVVGIAA YKPLSKDKAH CLLLYDDGSL
NIYSHTPNGS DSSTTLTAEQ TKKLGSSILS SRAYAGTKPE FPLDFFEKTT CITCDVKFNS
DTTKSSDSES IKQRLSSDDG YLESLTSAGF KVTISNPNPD IVMVGCRIHV GNTSASNIPS
EITIFHRVIK LDEGMRSWYD IPFTTAESLL ADEEFTIVVG RTFDGSSIPR IDSIEVYGRA
KDEFGWKEKM DAALDMEAHV LGGSSASGKS GKKAQTMQAA PIQEQVLADA LRILSRIYLL
CQPGFCTDTI DADMELNNLK CRSLLETIFQ SDREPLLHSA ACRVLQAVFP KKEIYYHVKD
TMRLLGVIKS LPSITSRIGV GGAASSWVTK EFIAQIHTVS KVAVHRKSNL ASFLETHGTE
LVDGLMQVFW GILDLDRPDT QRINSLVVPC VEFIYSYAEC LALHSNEKSG VSVAPAVALL
KKLLFAPYEA VQTSSSLAIS SRFLQVPFPK QTMIANDDAP DNHAKASAAS NSTTGNAQVM
IEEDPATSSV QYCCDGCSTV PILRRRWHCN ICPDFDLCET CYEILDADRL PAPHSRDHPM
SAIPIELDTF GGEGNEIHFS VDELTDSSVL QAPADRTIQT SPSSIHVLDA SESVDFHGSM
TEQRTVSISA SKRAINSLLL SRLIEELSGW METTAGTRAI PIMQLFYRLS SAVGGPFMDS
TKPENLDLEK FVKWLIDEIN ISKPFPAKTR CSFGEVSILV FMFFTLMFRN WHQPGTDGSH
SKSGGSSDLT EKGPVHVQVS TTTLQSSNDD HDKNEFASQL IRACSALRQQ SFLNYLMDIL
QQLVHVFKSS SINGEGGSSS SGCGSLLTVR RELPAGNFSP FFSDSYAKSH PTDLFMDYYK
LLLENTFRLV YSMVRPEKEK SADKDKSCKV PNTKDLKLDG YQDVLCSYIS NAHTTFVRRY
ARRLFLHLCG SKTHYYSVRD SWQYSHEVKK LHKIINKSGG FRNPVPYERS VKLIKCLSTL
CDVAASRPRN WQKFCLKHTD LLPFLMDNFY YFSEECIVQT LKLLNLAFYS GKDANHNAQK
TESGDIGSST RTGSQSSDSK KKRKGDDSSE GSSEKSCMDM EQAVVVFTGK DGDVLKRFVD
TFLLEWNSTS VRHEAKSVLF GLWYHAKSSF KENMLTTLLQ KVKYLPMYGQ NIIEYTDLMT
CLLGKANDST AKQSDTELLN KCLTSDVVSC IFDTLHSQNE LLANHPNSRI YNTLSCLVEF
DGYYLESEPC VTCSCPDVPY SRMKLESLKS ETKFTDNRII VKCTGSFTIQ SVTMNVYDAR
KSKSVKVLNL YYNNRPVTDL SELKNNWSLW KRAKSCHLTF NQTELKVEFP IPITACNFMI
ELDSFYENLQ ASSLESLQCP RCSRSVTDKH GICSNCHENA YQCRQCRNIN YENLDSFLCN
ECGYSKYGRF EFHFMAKPSF SFDNMENDDD MRKGLTAIES ESENAHRRYQ QLMGFKKPLI
KLVSSIGEQE IDSQQKDAVQ QMMVSLPGPT GKVNRKIALL GVLYGEKCKA AFDSVSKSVQ
TLQGLRRVLM TYLHQKNSND TDALPACSIP RSPSSCYGCS TTFVTQCLEL LQVLSKHATS
RKQLVSAGIL SELFENNIHQ GPRTARTLAR AVLSSFSEGD ADAVQELNNL IQKKVMYCLE
HHRSMDISQS TREELLLLSE TCALVDEFWE ARLRVAFQLL FSSIKVGAKH PAISEHIILP
CLRIISQACT PPKSDSGEKE PGMGKSSLMQ AKNDDTVGHS VTNLSTSKTQ SELSGKIPDG
SRRRQDISLL SYSEWESGAS YLDFVRRQYK VSQAVKGLQK TRHDSQKSDY LVLKYGLRWK
RRACRKSSKG DFSKFALGSW VSDLILSSCS QSIRSEICTL ISLLCPSNSS RQFQLLNLLM
SLLPRTLSAG ESAAEYFELL GTMIDTEASR LFLTVRGCLT TLCSLITKEV SNVESQERSL
SIDISQGFIL HKLVELLNKF LEIPNIRARF MSDNLLSDVL EAFLVIRGLV VQKTKLINDC
NRLLKDLLDS LLVESTANKR QFIRACISGL QKHVKEKKRR TSLFILEQLC NLICPVKPEP
VYLLILNKAH TQEEFIRGSM TRNPYSSAEI GPLMRDVKNK ICHQLDLIGL LEDDYGMELL
VAGNIISLDL SISQVYEQVW RKHHGQTQHS LSNASQLSAA ASSVRDCPPM TVTYRLQGLD
GEATEPMIKE LEDEREESQD PEVEFAIAGA VRECGGLEII LSMIQSLRED ELRSNQEELG
SVLNLLKYCC KIRENRCALL RLGALGLLLE TARRAFSVDA MEPAEGILLI VESLTMEANE
SDISIAQSVF TTTTEETGAG EEAKKIVLMF LERLCPPDGA KKSNKQQRNE EMVARILPNL
TYGEPAAMEA LVLHFEPYLM NWSEFDQLQK QHEENPKDET LSKNASMQRS AVENFVRVSE
SLKTSSCGER LKEIILEKGI TKAAVGHLRE SFASAGQASF RTSAEWTVGL KLPSIPLILS
MLKGLAKGDL PTQKCVDEED ILPLLHALEG VPGENEIGAR AENLLDTLAN KENNGDGFLA
EKIQELRHAT RDEMRRRALK KREMLLQGLG MRQEFASDGG RRIVVSQPII EGLDDVEEEE
DGLACMVCRE GYTLRPTDML GVYAFSKRVN LGATSSGSGR GDCVYTTVSH FNIIHYQCHQ
EAKRADAALK NPKKEWDGAT LRNNETLCNC IFPLRGPSVP PGQYTRCLDQ YWDQLNSLGR
ADGSRLRLLT YDIVLMLARF ATGASFSTDC KGGGRESNSR FLPFMIQMAS HLVDGSANQQ
RHVMAKAVTS YLSSSPSTPE SPVRLSALSG ARGGSGSSEE TVQFMMVNSL LSESYESWLQ
HRPAFLQRGI YHAYMQHKHG RSTLKLSADT SSSAVRSDEG SSADSNDSKR LFAIVQPMLV
YTGLIEQLQQ FFKKGKSSGT QKVGEKDGSS GGNLEAWEIM MKEKLGNMKE MLGFSKDVLS
WLEDMTSSED LQEAFDVMGA LPDVFSGGHT TCEDFVRAII HGAKS
