BIK_MOUSE
ID BIK_MOUSE Reviewed; 150 AA.
AC O70337;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Bcl-2-interacting killer;
DE AltName: Full=Apoptosis inducer NBK;
DE AltName: Full=Bik-like killer protein;
GN Name=Bik; Synonyms=Biklk, Blk, Nbk;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN APOPTOSIS, SUBCELLULAR LOCATION,
RP AND TISSUE SPECIFICITY.
RC TISSUE=Embryo;
RX PubMed=9525867; DOI=10.1074/jbc.273.14.7783;
RA Hegde R., Srinivasula S.M., Ahmad M., Fernandes-Alnemri T., Alnemri E.S.;
RT "Blk, a BH3-containing mouse protein that interacts with Bcl-2 and Bcl-xL,
RT is a potent death agonist.";
RL J. Biol. Chem. 273:7783-7786(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Accelerates programmed cell death. Binding to the apoptosis
CC repressors Bcl-X(L), BHRF1 or Bcl-2 suppresses this death-promoting
CC activity. {ECO:0000269|PubMed:9525867}.
CC -!- SUBUNIT: Interacts with RHBDL4/RHBDD1 (By similarity). Interacts with
CC BCL2L10/BCL-B (By similarity). {ECO:0000250|UniProtKB:Q13323}.
CC -!- SUBCELLULAR LOCATION: Endomembrane system {ECO:0000250}; Single-pass
CC membrane protein {ECO:0000250}. Mitochondrion membrane
CC {ECO:0000269|PubMed:9525867}; Single-pass membrane protein
CC {ECO:0000269|PubMed:9525867}. Note=Around the nuclear envelope, and in
CC cytoplasmic membranes. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in testis, kidney, liver, lung and heart.
CC {ECO:0000269|PubMed:9525867}.
CC -!- DOMAIN: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for
CC their pro-apoptotic activity and for their interaction with anti-
CC apoptotic members of the Bcl-2 family. {ECO:0000250}.
CC -!- PTM: Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced
CC cleavage is a necessary step prior its degradation by the proteosome-
CC dependent mechanism (By similarity). {ECO:0000250}.
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DR EMBL; AF048838; AAC40079.1; -; mRNA.
DR EMBL; AL583889; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH467427; EDL01409.1; -; Genomic_DNA.
DR CCDS; CCDS27703.1; -.
DR RefSeq; NP_031572.1; NM_007546.2.
DR RefSeq; XP_006520418.1; XM_006520355.3.
DR RefSeq; XP_006520419.1; XM_006520356.2.
DR RefSeq; XP_006520420.1; XM_006520357.3.
DR AlphaFoldDB; O70337; -.
DR ComplexPortal; CPX-310; BIK:BCL-w complex.
DR ELM; O70337; -.
DR STRING; 10090.ENSMUSP00000016902; -.
DR iPTMnet; O70337; -.
DR PhosphoSitePlus; O70337; -.
DR PaxDb; O70337; -.
DR PRIDE; O70337; -.
DR Antibodypedia; 13376; 559 antibodies from 37 providers.
DR DNASU; 12124; -.
DR Ensembl; ENSMUST00000016902; ENSMUSP00000016902; ENSMUSG00000016758.
DR Ensembl; ENSMUST00000230912; ENSMUSP00000155083; ENSMUSG00000016758.
DR GeneID; 12124; -.
DR KEGG; mmu:12124; -.
DR UCSC; uc007xbd.1; mouse.
DR CTD; 638; -.
DR MGI; MGI:1206591; Bik.
DR VEuPathDB; HostDB:ENSMUSG00000016758; -.
DR eggNOG; ENOG502TF5K; Eukaryota.
DR GeneTree; ENSGT00530000064453; -.
DR HOGENOM; CLU_139842_0_0_1; -.
DR InParanoid; O70337; -.
DR OrthoDB; 1487465at2759; -.
DR TreeFam; TF338339; -.
DR BioGRID-ORCS; 12124; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Bik; mouse.
DR PRO; PR:O70337; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; O70337; protein.
DR Bgee; ENSMUSG00000016758; Expressed in right kidney and 140 other tissues.
DR ExpressionAtlas; O70337; baseline and differential.
DR Genevisible; O70337; MM.
DR GO; GO:0097136; C:Bcl-2 family protein complex; ISO:MGI.
DR GO; GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005740; C:mitochondrial envelope; IDA:MGI.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051400; F:BH domain binding; ISO:MGI.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:MGI.
DR GO; GO:0008637; P:apoptotic mitochondrial changes; IDA:MGI.
DR GO; GO:0008584; P:male gonad development; IGI:MGI.
DR GO; GO:0031334; P:positive regulation of protein-containing complex assembly; ISO:MGI.
DR GO; GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; ISO:MGI.
DR GO; GO:0042981; P:regulation of apoptotic process; ISO:MGI.
DR GO; GO:0007283; P:spermatogenesis; IGI:MGI.
DR InterPro; IPR024579; Bcl2-int_killer.
DR PANTHER; PTHR15018; PTHR15018; 1.
DR Pfam; PF12201; bcl-2I13; 1.
PE 1: Evidence at protein level;
KW Apoptosis; Membrane; Mitochondrion; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..150
FT /note="Bcl-2-interacting killer"
FT /id="PRO_0000419202"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 127..148
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255"
FT MOTIF 51..65
FT /note="BH3"
FT /evidence="ECO:0000250"
FT SITE 143..144
FT /note="Cleavage; by RHBDL4/RHBDD1"
FT /evidence="ECO:0000250"
SQ SEQUENCE 150 AA; 16901 MW; 120CB6BAA4612788 CRC64;
MSEARLMARD VIKTVPHDQV PQPPVASETP SMKEPVRDVD LMECVEGRNQ VALRLACIGD
EMDLCLRSPR LVQLPGIAIH RLAVTYSRTG VRGIFRSLIR SLTNLRENIW SWRVLTPGAW
VSPDQDPGQL FPMVLLVFLL LGGAWYLQLQ
